| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,587,162 | G→A | 10.2% | E304E (GAG→GAA) | acrD → | aminoglycoside/multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,587,162 | 0 | G | A | 10.2% | 114.0 / 4.0 | 39 | E304E (GAG→GAA) | acrD | aminoglycoside/multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base G (21/14); new base A (2/2); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
AATGGTAAGCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCG > AP009048/2587094‑2587228 | aaTGGTAAGCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGc < 1:4071018/70‑1 (MQ=255)aaTGGTAAGCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCt > 1:1770020/1‑71 (MQ=255) ggTAAGCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGt > 1:1436032/1‑71 (MQ=255) ggTAAGCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGt > 1:29748/1‑71 (MQ=255) gccagccTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGc < 1:654769/62‑1 (MQ=255) ccagccTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCaa < 1:1952088/71‑1 (MQ=255) agccTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGt < 1:2824185/63‑1 (MQ=255) cTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGtc > 1:1462227/1‑71 (MQ=255) ccGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGtctc > 1:2571932/1‑71 (MQ=255) cGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAACTGGTGCTCAATCGtctc < 1:408580/70‑1 (MQ=255) ggggTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAACTGGTGCTCAATCGTCTCGACGAGc < 1:350259/70‑1 (MQ=255) gggTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTg < 1:1309337/71‑1 (MQ=255) gTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGgc < 1:4105172/71‑1 (MQ=255) tAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGgcg > 1:234501/1‑71 (MQ=255) aaaaCTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGgcg > 1:1217767/1‑70 (MQ=255) aaaaCTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGgcg > 1:1331421/1‑70 (MQ=255) aaCTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAg < 1:1445110/71‑1 (MQ=255) cTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAACTGGTGCTCAATCGt > 1:3449742/1‑51 (MQ=255) ccTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTc < 1:1318200/71‑1 (MQ=255) cTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTcc > 1:2541011/1‑71 (MQ=255) tCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTAt < 1:442954/66‑1 (MQ=255) cGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCg > 1:3344665/1‑70 (MQ=255) cGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCg > 1:1400641/1‑70 (MQ=255) gcTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATAGTCTCGACGAGCTGGCGCAGTATTTCCCGCATg > 1:2425782/1‑70 (MQ=255) aaCGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCTGCATGGACt > 1:2833043/1‑71 (MQ=255) aaCGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACt > 1:2959332/1‑71 (MQ=255) aCGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTg > 1:4556481/1‑71 (MQ=255) aCGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTg < 1:3397609/71‑1 (MQ=255) cGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTgg < 1:4342862/71‑1 (MQ=255) cGAAATGGCGACAGCGGAACTGGTGCTCAATCGtct > 1:3680992/1‑36 (MQ=255) gAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTg > 1:1130574/1‑45 (MQ=255) aaaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTg > 1:142718/1‑44 (MQ=255) aaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGaa > 1:196443/1‑70 (MQ=255) cGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGc < 1:1649693/54‑1 (MQ=255) aGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTg > 1:3153759/1‑70 (MQ=255) aGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTg > 1:3148497/1‑70 (MQ=255) cGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACaa > 1:3733280/1‑64 (MQ=255) cGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCg < 1:3767523/71‑1 (MQ=255) ggAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTgg < 1:292613/56‑1 (MQ=255) | AATGGTAAGCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCG > AP009048/2587094‑2587228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |