| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,065,341 | G→T | 10.1% | I163I (ATC→ATA) | ygfI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,065,341 | 0 | G | T | 10.1% | 220.2 / 6.8 | 69 | I163I (ATC→ATA) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (26/36); new base T (3/4); total (29/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAA > AP009048/3065271‑3065406 | gctcttgctcttGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTg < 1:1377457/71‑1 (MQ=255)gctcttgctcttGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTGCACTg < 1:1352528/71‑1 (MQ=255) ctcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:2279980/71‑1 (MQ=255) tcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:3529125/1‑70 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:809566/1‑69 (MQ=255) cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1913061/1‑69 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:3932575/70‑1 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt > 1:3184130/1‑70 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:332175/70‑1 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt > 1:1203765/1‑70 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:1014182/70‑1 (MQ=255) ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:3876210/70‑1 (MQ=255) ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:1681244/70‑1 (MQ=255) ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:3572862/70‑1 (MQ=255) ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTc < 1:424258/70‑1 (MQ=255) tcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAg > 1:4611175/1‑71 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:3838615/1‑71 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:1986024/1‑71 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:1427651/68‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg > 1:1104134/1‑68 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGttt > 1:924124/1‑71 (MQ=255) ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc > 1:1675762/1‑69 (MQ=255) ctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt > 1:2904928/1‑71 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:3943521/57‑1 (MQ=255) tCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc > 1:22082/1‑71 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:294553/1‑60 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:1694210/1‑71 (MQ=255) aTAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:3917386/50‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg < 1:4295985/71‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGACTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTg < 1:2720846/71‑1 (MQ=255) aaCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:3146317/62‑1 (MQ=255) aaCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg > 1:1563466/1‑70 (MQ=255) cGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAAc < 1:3601514/71‑1 (MQ=255) ggCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt < 1:2383971/49‑1 (MQ=255) gCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTc > 1:3929438/1‑71 (MQ=255) gagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGt < 1:1215688/71‑1 (MQ=255) gagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGt < 1:3301759/71‑1 (MQ=255) agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:4144912/71‑1 (MQ=255) agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg > 1:2424210/1‑71 (MQ=255) gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc > 1:3062958/1‑53 (MQ=255) gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:1993708/70‑1 (MQ=255) gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg > 1:476674/1‑70 (MQ=255) acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1238111/1‑37 (MQ=255) acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:2693570/56‑1 (MQ=255) cgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa > 1:2775108/1‑70 (MQ=255) cgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:4109007/1‑45 (MQ=255) aaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACAt > 1:4525425/1‑71 (MQ=255) aGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGc < 1:705165/71‑1 (MQ=255) gCCTAATCGCAGCGGTTGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCt < 1:4586514/71‑1 (MQ=255) gCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCt < 1:2748749/71‑1 (MQ=255) ccTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:2705631/41‑1 (MQ=255) cTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAg < 1:3256789/60‑1 (MQ=255) tAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgtg > 1:2873045/1‑71 (MQ=255) aaTCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCGGATACATGCTGTgg > 1:3211094/1‑71 (MQ=255) tCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:2778509/36‑1 (MQ=255) tCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCg < 1:3504381/51‑1 (MQ=255) tggtAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGataa < 1:636245/68‑1 (MQ=255) tggtAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgt < 1:3117979/58‑1 (MQ=255) tAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGACCTCGTGCAGATACATGCTGTggg < 1:1629056/58‑1 (MQ=255) tAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtg < 1:1820927/54‑1 (MQ=255) aGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATAc > 1:1003155/1‑47 (MQ=255) gTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTc < 1:4493143/36‑1 (MQ=255) gTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaa > 1:2074279/1‑67 (MQ=255) gTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCt < 1:3972314/71‑1 (MQ=255) ttCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:2639324/40‑1 (MQ=255) ttCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:3022154/44‑1 (MQ=255) ttCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATc > 1:1942308/1‑69 (MQ=255) tCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaaa < 1:4480405/66‑1 (MQ=255) cACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTaa < 1:1169324/70‑1 (MQ=255) cTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATg < 1:3352663/44‑1 (MQ=255) cTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgt < 1:4372834/48‑1 (MQ=255) | GCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAA > AP009048/3065271‑3065406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |