| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,139,938 | C→T | 6.0% | R2H (CGT→CAT) | hybD ← | predicted maturation element for hydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,139,938 | 0 | C | T | 6.0% | 217.7 / 3.0 | 67 | R2H (CGT→CAT) | hybD | predicted maturation element for hydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base C (30/33); new base T (2/2); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
CGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGCC > AP009048/3139868‑3140005 | cGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAACCCTAAGACTAAAATAc > 1:4500805/1‑71 (MQ=255) cTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg > 1:1182108/1‑70 (MQ=255) ttCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa > 1:3497081/1‑71 (MQ=255) ttCGACAATACGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATAcaca > 1:3477981/1‑71 (MQ=255) tCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt > 1:3521472/1‑71 (MQ=255) tCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt > 1:3103240/1‑71 (MQ=255) aCAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa > 1:336660/1‑70 (MQ=255) aaTCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa > 1:3222932/1‑68 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc > 1:35230/1‑71 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt < 1:1405399/71‑1 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt < 1:1987794/71‑1 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt < 1:256296/71‑1 (MQ=255) cacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCa < 1:4605293/71‑1 (MQ=255) acCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCAc > 1:510229/1‑71 (MQ=255) cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc < 1:3829441/58‑1 (MQ=255) gATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGa < 1:305515/71‑1 (MQ=255) gCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAcc < 1:1307490/71‑1 (MQ=255) cTTCATAGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAATACAGAACCTTCACTGAAACCa < 1:1837988/71‑1 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaaa > 1:4252680/1‑66 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaa < 1:22920/65‑1 (MQ=255) ggTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGtt < 1:1497788/71‑1 (MQ=255) gTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa < 1:3305350/45‑1 (MQ=255) gTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTg < 1:4123052/71‑1 (MQ=255) gTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:1209240/1‑71 (MQ=255) aGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGcc < 1:37091/70‑1 (MQ=255) aGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCg > 1:1980972/1‑71 (MQ=255) aGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCg > 1:1583229/1‑71 (MQ=255) gCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt < 1:3250100/71‑1 (MQ=255) cAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCa > 1:757462/1‑49 (MQ=255) cAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAc > 1:4601034/1‑56 (MQ=255) cAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt > 1:1182979/1‑70 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAc > 1:4134248/1‑42 (MQ=255) aaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGa > 1:3438620/1‑39 (MQ=255) aaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAcc < 1:1720252/54‑1 (MQ=255) atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa < 1:3812463/71‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:4459544/70‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:2355064/70‑1 (MQ=255) ccGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACt < 1:3911203/71‑1 (MQ=255) cGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg < 1:470935/52‑1 (MQ=255) cGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:3659900/66‑1 (MQ=255) gACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTAc < 1:3190707/71‑1 (MQ=255) aCCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc > 1:3186559/1‑58 (MQ=255) cccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:984808/62‑1 (MQ=255) ccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt < 1:2016765/71‑1 (MQ=255) tAAGACTAAACTACGCATTACAGAACCTTCACTGAAACCACtt < 1:291498/43‑1 (MQ=255) tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:3140553/1‑71 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGaa > 1:1688664/1‑36 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGaa > 1:4319623/1‑36 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGa < 1:1357772/35‑1 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:4326464/1‑70 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGt > 1:1650138/1‑45 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:3265330/58‑1 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:3592014/1‑69 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:2135157/1‑69 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:173141/1‑68 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCg > 1:1536669/1‑71 (MQ=255) aCTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGca > 1:3524301/1‑71 (MQ=255) aCTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAcc > 1:2594205/1‑68 (MQ=255) cTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt > 1:455131/1‑47 (MQ=255) cTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGcac < 1:1552751/71‑1 (MQ=255) aaaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTa < 1:2178436/57‑1 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAgg > 1:3312946/1‑71 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAgg > 1:3357933/1‑71 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCg < 1:2622073/36‑1 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTAc < 1:2683654/56‑1 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGc < 1:1827922/71‑1 (MQ=255) aTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCa < 1:2087347/44‑1 (MQ=255) aTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:3723369/49‑1 (MQ=255) aTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGcc < 1:1637165/71‑1 (MQ=255) cGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAg < 1:1410821/42‑1 (MQ=255) | CGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGCC > AP009048/3139868‑3140005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |