| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,893,021 | G→T | 10.4% | intergenic (‑142/+97) | ygdL ← / ← mltA | conserved hypothetical protein/membrane‑bound lytic murein transglycosylase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,893,021 | 0 | G | T | 10.4% | 216.6 / 8.5 | 67 | intergenic (‑142/+97) | ygdL/mltA | conserved hypothetical protein/membrane‑bound lytic murein transglycosylase A |
| Reads supporting (aligned to +/- strand): ref base G (29/31); new base T (5/2); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
AGGCCTACATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCACCCTGTCA > Exported/1892954‑1893086 | aGGCCTACATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:2160485/1‑71 (MQ=255) ggCCTACATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:3309532/71‑1 (MQ=255) ggCCTACATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:2287617/71‑1 (MQ=255) gCCTACATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:3599574/1‑71 (MQ=255) aCATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:1840333/1‑71 (MQ=255) cATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:555757/1‑61 (MQ=255) cATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAt > 1:1973786/1‑71 (MQ=255) aTAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATg < 1:648271/71‑1 (MQ=255) aCTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaac < 1:2654545/71‑1 (MQ=255) aCTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaac < 1:449012/71‑1 (MQ=255) aCTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa > 1:321211/1‑70 (MQ=255) cTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg < 1:1987389/62‑1 (MQ=255) cTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaaca < 1:87538/71‑1 (MQ=255) gCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:1541856/1‑59 (MQ=255) tatTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCAcgccgc < 1:3637515/48‑1 (MQ=255) tatTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCAcgccgc < 1:1425275/48‑1 (MQ=255) ttttGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACt > 1:3407756/1‑69 (MQ=255) ttGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAg < 1:1807654/70‑1 (MQ=255) ttGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGt > 1:2665127/1‑71 (MQ=255) tGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGt > 1:291792/1‑70 (MQ=255) tGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGt < 1:692108/70‑1 (MQ=255) gATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaa > 1:871088/1‑71 (MQ=255) gATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCa < 1:2241906/70‑1 (MQ=255) aTTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaat < 1:3165246/71‑1 (MQ=255) tttGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaata < 1:2888235/71‑1 (MQ=255) ttGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataa > 1:612414/1‑71 (MQ=255) ttGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataa > 1:3605708/1‑71 (MQ=255) ttGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataa > 1:1507320/1‑71 (MQ=255) ttGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataa > 1:1226791/1‑71 (MQ=255) tGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaa > 1:182145/1‑52 (MQ=255) tGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaa > 1:1824763/1‑52 (MQ=255) tGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaa > 1:638673/1‑52 (MQ=255) tGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataat > 1:2420079/1‑71 (MQ=255) gCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataat < 1:2544112/70‑1 (MQ=255) gCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCaataat > 1:1682232/1‑70 (MQ=255) aTGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAg < 1:1737630/71‑1 (MQ=255) tGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAg > 1:3224780/1‑70 (MQ=255) gATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAcgcg < 1:2367584/51‑1 (MQ=38) tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGAtgt < 1:1415833/70‑1 (MQ=255) tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGAtgt < 1:2127613/70‑1 (MQ=255) tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGAtgt > 1:1535247/1‑70 (MQ=255) tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGAtgt < 1:1785165/70‑1 (MQ=255) tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGAtgt > 1:1740018/1‑70 (MQ=255) tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGa < 1:3459399/70‑1 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTAc < 1:391895/71‑1 (MQ=255) cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTAc > 1:2529795/1‑71 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCt > 1:2921951/1‑71 (MQ=255) aGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCa > 1:2018823/1‑71 (MQ=255) ggCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCa < 1:118532/70‑1 (MQ=255) ggCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCAc < 1:1809035/71‑1 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCAcc > 1:1190688/1‑71 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCAcc > 1:2259185/1‑71 (MQ=255) gTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCACCCt < 1:2005986/71‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAg < 1:785736/55‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAg < 1:688947/55‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAg < 1:224638/55‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAg < 1:3159816/55‑1 (MQ=255) gaaCGCCTCATCCGGCATGAACAACGCGCACTTAGTCAATAATCa < 1:649795/43‑1 (MQ=18) gaaCGCCTCATCCGGCATGAACAACGCGCACTTAGTCAATAATCa < 1:1296370/43‑1 (MQ=18) cACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGt > 1:732381/1‑55 (MQ=255) aCGCCTTATCCGGCCTGAACACCGCGCACTTAGTCAATAATCAgg > 1:851825/1‑45 (MQ=21) aCGCCTTATCCGGCCTGAACAACGCGCACTTAGTCAATAATCAgg > 1:2784830/1‑45 (MQ=18) aCGCCTTATCCGGCCTGAACAACGCGCACTTAGTCAATAATCAgg > 1:1122311/1‑45 (MQ=18) aCGCCTTATCCGGCCTGAACAACGCGCACTTAGTCAATAATCAgg > 1:1695674/1‑45 (MQ=18) aCGCCTTATCCGGCCTGAACAACGCGCACTTAGTCAATAATCAgg > 1:1078232/1‑45 (MQ=18) aCGCCGCATCCGGCATGAACAACGCGCACTTAGTCatta < 1:1333114/39‑1 (MQ=255) aCGCCGCATCCGGCATGAACAACGCGCACTTAGTCaata < 1:152348/39‑1 (MQ=255) aCGCCGCATCCGGCATGAACAACGCGCACTTAGTCaata < 1:1825100/39‑1 (MQ=255) aCGCCGCATCCGGCATGAACAACGCGCACTTAGTCaata < 1:2317360/39‑1 (MQ=255) aCGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCACCCTGTCa > 1:3151952/1‑71 (MQ=255) cgcATCCGGCATGAACAACGCGCACTTAGTCaataa < 1:2301415/36‑1 (MQ=255) cgcATCCGGCATGAACAACGCGCACTTAGTCaataa < 1:3397048/36‑1 (MQ=255) cgcATCCGGCATGAACAACGCGCACTTAGTCaataa < 1:2548838/36‑1 (MQ=255) cgcATCCGGCATGAACAACGCGCACTTAGTCaataa < 1:273030/36‑1 (MQ=255) cgcATCCGGCATGAACAACGCGCACTTAGTCaataa < 1:863779/36‑1 (MQ=255) cgcATCCGGCATGAACAACGCGCACTTAGTCaataa < 1:2840644/36‑1 (MQ=255) | AGGCCTACATAACTCCTGCAATATTTTGGATTTGCATGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACTTAGTCAATAATCAGGATGTGAGGTTACCCCTCACCCTGTCA > Exported/1892954‑1893086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |