| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,589,325 | A→G | 11.0% | intergenic (‑1/+798) | gadE ← / ← hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,589,325 | 0 | A | G | 11.0% | 307.6 / 25.9 | 109 | intergenic (‑1/+798) | gadE/hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (48/49); new base G (5/7); total (53/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
TTTCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCG > Exported/2589255‑2589394 | tttCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATa < 1:2251054/71‑1 (MQ=255) cGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATGAc < 1:3597359/70‑1 (MQ=255) gTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACtt > 1:303059/1‑71 (MQ=255) gTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACtt > 1:603168/1‑71 (MQ=255) aTTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCt < 1:3286294/71‑1 (MQ=255) aTTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCt > 1:1374223/1‑71 (MQ=255) tCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt > 1:2103224/1‑70 (MQ=255) tCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTa < 1:2743977/68‑1 (MQ=255) tCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGcc > 1:1935815/1‑71 (MQ=255) cAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTa < 1:2248250/67‑1 (MQ=255) cTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAg < 1:2452770/65‑1 (MQ=255) aaaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGtt > 1:3609027/1‑71 (MQ=255) aaaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGtt < 1:336098/71‑1 (MQ=255) aaaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGtt < 1:2561938/71‑1 (MQ=255) aaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGttt > 1:3735084/1‑71 (MQ=255) aaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGtt > 1:2391432/1‑70 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCg < 1:1216325/71‑1 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCg < 1:2547445/71‑1 (MQ=255) gCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGa < 1:1042596/71‑1 (MQ=255) gCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGa < 1:233206/71‑1 (MQ=255) cccTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:2405556/71‑1 (MQ=255) ccTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCTTTTCGaa < 1:715359/70‑1 (MQ=255) cTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATaa < 1:2393148/41‑1 (MQ=255) cTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:1559379/69‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTg > 1:3209083/1‑42 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTg > 1:3413620/1‑42 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt < 1:2778124/47‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:3681084/66‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATg < 1:136665/70‑1 (MQ=255) aaaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTg > 1:1322958/2‑42 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATa < 1:2178241/36‑1 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATa < 1:185935/36‑1 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAg < 1:1865585/49‑1 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATc > 1:2979093/1‑57 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATc > 1:3815764/1‑57 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGtt > 1:71550/1‑60 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATg < 1:120551/68‑1 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTa > 1:1115379/1‑47 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTa > 1:3052418/1‑47 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:719496/1‑71 (MQ=255) gaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATcc > 1:1745931/1‑71 (MQ=255) gaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATcc > 1:1007335/1‑71 (MQ=255) aaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCt < 1:2541450/41‑1 (MQ=255) aaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGtt < 1:1897743/49‑1 (MQ=255) aaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGtt < 1:2909818/49‑1 (MQ=255) aaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa < 1:409017/66‑1 (MQ=255) aaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa < 1:2673968/66‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTcc < 1:960452/38‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTcc < 1:1310498/38‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTcc < 1:3609693/38‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTcc < 1:3813453/38‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCttt < 1:986401/71‑1 (MQ=255) agaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAg < 1:1571675/41‑1 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattatt < 1:2863900/70‑1 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattatt > 1:2597306/1‑70 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta < 1:3086465/68‑1 (MQ=255) cGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATcc > 1:514776/1‑58 (MQ=255) cGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCa > 1:3450728/1‑64 (MQ=255) cGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTa > 1:2757202/1‑71 (MQ=255) cGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTa > 1:1690022/1‑71 (MQ=255) gTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTa > 1:3814560/1‑70 (MQ=255) cATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACa > 1:968349/1‑70 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCAt < 1:1308316/52‑1 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCAt < 1:2936760/52‑1 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCAt < 1:1719989/52‑1 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCt > 1:810370/1‑55 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCt > 1:921561/1‑55 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCt > 1:3748898/1‑55 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCt > 1:3674740/1‑55 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCt > 1:3606508/1‑55 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATc > 1:2655793/1‑71 (MQ=255) tGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCAt < 1:1394735/51‑1 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta > 1:918485/1‑60 (MQ=255) gaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTg < 1:3759443/71‑1 (MQ=255) gaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTg < 1:1847080/71‑1 (MQ=255) gaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTg > 1:2710581/1‑71 (MQ=255) gaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTg > 1:2978681/1‑71 (MQ=255) aaaaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta > 1:3317459/1‑58 (MQ=255) taaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:2105352/37‑1 (MQ=255) aaaTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTcc > 1:3748636/1‑71 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:2361051/1‑39 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCAtac > 1:2560418/1‑44 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCttt < 1:1022552/49‑1 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCttt < 1:2466963/49‑1 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCttt < 1:9577/49‑1 (MQ=255) aTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTc < 1:2286871/50‑1 (MQ=255) aTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTc < 1:2302227/50‑1 (MQ=255) aTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTc < 1:2622679/50‑1 (MQ=255) aTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTc < 1:1118038/50‑1 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCg > 1:742295/1‑70 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCg > 1:3278766/1‑70 (MQ=255) cATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtttt > 1:1089920/1‑47 (MQ=255) cATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtttt > 1:1438015/1‑47 (MQ=255) cATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtttt > 1:3042678/1‑47 (MQ=255) cATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtttt > 1:3150457/1‑47 (MQ=255) cATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtttt > 1:1563698/1‑47 (MQ=255) aTAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATc < 1:2387141/71‑1 (MQ=255) aTAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATc < 1:2244315/71‑1 (MQ=255) tAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCt > 1:1578756/1‑60 (MQ=255) tAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCAGAATCg > 1:3072770/1‑71 (MQ=255) | TTTCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCG > Exported/2589255‑2589394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |