| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,676,291 | G→T | 5.5% | V14F (GTT→TTT) | bioH → | carboxylesterase of pimeloyl‑CoA synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,676,291 | 0 | G | T | 5.5% | 311.7 / 2.5 | 91 | V14F (GTT→TTT) | bioH | carboxylesterase of pimeloyl‑CoA synthesis |
| Reads supporting (aligned to +/- strand): ref base G (43/42); new base T (2/3); total (45/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
ATCCTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAGG > Exported/2676221‑2676354 | aTCCTTGTAAGTCAAAAGAGAACAATAGCGTATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATg > 1:3165985/1‑71 (MQ=255)aTCCTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATg < 1:2310552/71‑1 (MQ=255) ccTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGtt > 1:1785765/1‑71 (MQ=255) cTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGtt > 1:2529761/1‑70 (MQ=255) cTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTc < 1:2085576/71‑1 (MQ=255) cTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTc < 1:88037/71‑1 (MQ=255) gTAAGTCAAAATAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCAt < 1:1625409/70‑1 (MQ=255) aaGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATc > 1:1371713/1‑69 (MQ=255) gTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTtgt < 1:2761006/71‑1 (MQ=255) cAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGtgc > 1:954160/1‑71 (MQ=255) cAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGtgc > 1:3794517/1‑71 (MQ=255) aaaaGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGtgct > 1:2771015/1‑71 (MQ=255) aaGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGtgctg > 1:2251576/1‑70 (MQ=255) agaACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGtgctgctgc < 1:165339/71‑1 (MQ=255) gaACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCa > 1:2763242/1‑71 (MQ=255) aaCAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATc < 1:1622227/57‑1 (MQ=255) aaCAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATc < 1:929041/57‑1 (MQ=255) cAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCtt > 1:2051605/1‑57 (MQ=255) cAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCtt > 1:2054677/1‑57 (MQ=255) cAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCtt > 1:1136000/1‑57 (MQ=255) tAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATg < 1:2158866/71‑1 (MQ=255) tAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATg < 1:2024396/71‑1 (MQ=255) aGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCtt > 1:2433340/1‑53 (MQ=255) gCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATggg < 1:482040/71‑1 (MQ=255) gCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATgcg < 1:1630441/71‑3 (MQ=255) cGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATggg > 1:507324/1‑70 (MQ=255) cGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTAATCTTGTGCTGCTGCACGGATggg > 1:1973714/1‑70 (MQ=255) gATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTtgt < 1:2326506/51‑1 (MQ=255) tGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTg < 1:2041330/71‑1 (MQ=255) tGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTg < 1:396545/71‑1 (MQ=255) tGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTg < 1:2601975/71‑1 (MQ=255) gAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACt > 1:3109266/1‑69 (MQ=255) aaTAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATc < 1:1574783/43‑1 (MQ=255) aaTAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCAc < 1:1458360/57‑1 (MQ=255) aaTAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGaa < 1:2416562/71‑1 (MQ=255) aaTAACATCTGGTGGCAGACCAAAGGTCAGGGGAATCTTCATc < 1:495068/43‑1 (MQ=255) aaTAACATCTGGTCGCAGACCAAAGGTCAGGGGAATGTTCATc < 1:3846779/43‑1 (MQ=255) aTAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAAt < 1:2991889/71‑1 (MQ=255) taccATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATg > 1:1653236/4‑71 (MQ=255) tAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATc < 1:1340051/41‑1 (MQ=255) tAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATg > 1:3219218/1‑71 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCa > 1:1407123/1‑52 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCa > 1:422402/1‑52 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGcc > 1:2619538/1‑71 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGcc > 1:2729436/1‑71 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGcc > 1:1296954/1‑71 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGcc > 1:1285813/1‑71 (MQ=255) aCATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGc > 1:3447175/1‑70 (MQ=255) aCATCTGGTGGCAGACCAAAGGTAAGGGGAATGTTCAta > 1:628744/1‑38 (MQ=38) tggtggCAGACCAAAGGTCAGGGGAATGTTCATCTtg < 1:62432/37‑1 (MQ=255) tggtggCAGACCAAAGGTCAGGGGAATGTTCATCTtg < 1:3127261/37‑1 (MQ=255) tggtggCAGACCAAAGGTCAGGGGAATGTTCATCTtg < 1:2748923/37‑1 (MQ=255) tggtggCAGACCAAAGGTCAGGGGAATGTTCATCTtg < 1:1363504/37‑1 (MQ=255) tggtggCAGACCAAAGGTCAGGGGAATGTTCATCTtg < 1:195460/37‑1 (MQ=255) ggtggCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAgtg > 1:2157623/1‑71 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTtgtg > 1:2808732/1‑37 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTtgtg > 1:2959393/1‑37 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTtgtg > 1:1062140/1‑37 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTtgtg > 1:1124969/1‑37 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTtgtg > 1:880164/1‑37 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTtgtg > 1:3364286/1‑37 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGaa < 1:679907/67‑1 (MQ=255) gtggCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGaa < 1:3413995/67‑1 (MQ=255) tggCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGcc < 1:2810872/63‑1 (MQ=255) tggCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGtgtg > 1:3717864/1‑71 (MQ=255) aGACCAAAGGTCAGGGGAATGTTCATCTTGtgctgctg > 1:919490/1‑38 (MQ=255) aGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGaa > 1:1489877/1‑62 (MQ=255) aCCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGaa > 1:3422470/1‑53 (MQ=255) aCCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTg > 1:3766562/1‑71 (MQ=255) aCCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTg < 1:1024386/71‑1 (MQ=255) ccAAAGGTCAGGGGAATTTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTg > 1:2498850/1‑70 (MQ=255) ccAAAGGTCAGGGGAATTTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTg > 1:3744385/1‑70 (MQ=255) cAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGa > 1:3720333/1‑40 (MQ=255) cAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGa > 1:2651341/1‑40 (MQ=255) cAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGa > 1:469424/1‑40 (MQ=255) cAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGa > 1:3191582/1‑40 (MQ=255) cAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCa > 1:2670296/1‑71 (MQ=255) cAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCa < 1:1702424/71‑1 (MQ=255) aaGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCAtt < 1:3229085/71‑1 (MQ=255) aaGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCAtt > 1:633517/1‑71 (MQ=255) aaGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCAtt < 1:409593/71‑1 (MQ=255) aaGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCAtt < 1:2438770/71‑1 (MQ=255) aaGGTCAGGGGAATGCTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCAtt < 1:3257876/71‑1 (MQ=255) aGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTg < 1:407253/71‑1 (MQ=255) gTCAGGGGAATTTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGAc < 1:498749/71‑1 (MQ=255) gTCAGGGGAATTTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGAc < 1:2223409/71‑1 (MQ=255) gTCAGGGGAATTTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGAc < 1:2768800/71‑1 (MQ=255) aGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGa < 1:1547975/70‑1 (MQ=255) ggggAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAgg < 1:2143023/71‑1 (MQ=255) ggggAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAgg < 1:586260/71‑1 (MQ=255) ggggAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAgg < 1:3082574/71‑1 (MQ=255) ggggAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAgg < 1:2781242/71‑1 (MQ=255) gggAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAgg < 1:2214349/70‑1 (MQ=255) | ATCCTTGTAAGTCAAAAGAGAACAATAGCGGATGAATAACATCTGGTGGCAGACCAAAGGTCAGGGGAATGTTCATCTTGTGCTGCTGCACGGATGGGGACTGAATGCCGAAGTGTGGCGTTGCATTGACGAGG > Exported/2676221‑2676354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |