| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 231,008 | T→G | 6.4% | intergenic (‑127/‑114) | yafC ← / → yafD | predicted DNA‑binding transcriptional regulator/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 231,008 | 0 | T | G | 6.4% | 163.5 / 6.6 | 63 | intergenic (‑127/‑114) | yafC/yafD | predicted DNA‑binding transcriptional regulator/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (38/21); new base G (2/2); total (40/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TCAGGATGCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTC > AP009048/230938‑231077 | tCAGGATGCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACt > 1:2385274/1‑71 (MQ=255) aGGATGCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTg > 1:3048742/1‑71 (MQ=255) aTGCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCtt < 1:2488257/71‑1 (MQ=255) aTGCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCt > 1:4674490/1‑70 (MQ=255) gCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTa > 1:1523056/1‑70 (MQ=255) cGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTAc < 1:640310/70‑1 (MQ=255) tGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACtt < 1:1426492/62‑1 (MQ=255) tGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATa < 1:4993042/71‑1 (MQ=255) atatGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATaa < 1:2826497/70‑1 (MQ=255) atatGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATaa > 1:3750984/1‑70 (MQ=255) atGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACaa > 1:59144/1‑71 (MQ=255) atGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACa > 1:5156349/1‑70 (MQ=255) aTGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATa > 1:4679280/1‑62 (MQ=255) tGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACa < 1:3596644/71‑1 (MQ=255) gCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACaa > 1:991693/1‑71 (MQ=255) gCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACaa > 1:4438344/1‑71 (MQ=255) cGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCAAGTGCTTACa < 1:418708/57‑1 (MQ=39) aTTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTg > 1:3016972/1‑71 (MQ=255) tttAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGc > 1:4958473/1‑71 (MQ=255) tttAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGc > 1:3030507/1‑71 (MQ=255) ttAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACAt > 1:2242889/1‑54 (MQ=255) ttAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCt > 1:5062722/1‑71 (MQ=255) aTGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCAAGTGCTTACac < 1:3915449/51‑2 (MQ=38) tctcCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTg > 1:1613826/1‑69 (MQ=255) ctcCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAAtt > 1:1596254/1‑60 (MQ=255) ctcCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAAtt > 1:3200645/1‑60 (MQ=255) ccATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACaa > 1:264200/1‑56 (MQ=255) ccATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGtt > 1:1375991/1‑65 (MQ=255) cATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTa < 1:1968415/40‑1 (MQ=255) cATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAagag > 1:863238/1‑71 (MQ=255) cATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAag < 1:3486183/69‑1 (MQ=255) aTAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACa > 1:2301146/1‑46 (MQ=255) tAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACAt < 1:4065742/41‑1 (MQ=255) tAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGa < 1:4119541/64‑1 (MQ=255) aaTGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACAt < 1:1963799/40‑1 (MQ=255) aaTGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAAtt > 1:1229223/1‑54 (MQ=255) aaTGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAAtt > 1:4241346/1‑54 (MQ=255) aaTGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGc > 1:4888372/1‑56 (MQ=255) aaTGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGt > 1:4978691/1‑69 (MQ=255) aTGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAg > 1:3995355/1‑71 (MQ=255) cAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTa > 1:5074523/1‑69 (MQ=255) aaTTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATc > 1:5048291/1‑71 (MQ=255) aTTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATc < 1:1659075/70‑1 (MQ=255) ttCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGc > 1:3642205/1‑45 (MQ=255) ttCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATccc > 1:4830033/1‑71 (MQ=255) cTGACCGGTTTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCta > 1:2986235/1‑71 (MQ=255) tGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCta > 1:1591323/1‑70 (MQ=255) aCCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATaa < 1:484766/71‑1 (MQ=255) ccGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCtata < 1:2806203/69‑1 (MQ=255) ccGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATaa > 1:5076693/1‑70 (MQ=255) cGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACa < 1:1711017/71‑1 (MQ=255) gtgtAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAg > 1:3504920/1‑70 (MQ=255) gtgtAAGCACTTGCTTACATAACAATATACAATAGCTCGTTGAAaga > 1:4219753/1‑47 (MQ=255) atgtAAGCAAGTGCTTACACAACAATATACAATTGCTCGTTGAAaga > 1:1894433/2‑47 (MQ=25) atgtAAGCAAGTGCTTACACAACAATATACAATTGCTCGTTGAAaga > 1:1522256/2‑47 (MQ=25) gtAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTaa < 1:1085154/54‑1 (MQ=255) gtAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACa > 1:1221296/1‑67 (MQ=255) tAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAaga < 1:1750761/44‑1 (MQ=255) tAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTaaa < 1:1358451/54‑1 (MQ=255) tAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAg < 1:1101318/70‑1 (MQ=255) aaGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCACTATAACAGTAGa > 1:4706063/1‑70 (MQ=255) aGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTg < 1:3265520/45‑1 (MQ=255) gCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGaa < 1:5128778/69‑1 (MQ=255) gCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAAcc > 1:493722/1‑71 (MQ=255) cTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTc < 1:2377671/71‑1 (MQ=255) | TCAGGATGCGTGATATGTCATGCGATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTC > AP009048/230938‑231077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |