| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,093,686 | T→C | 100% | V130A (GTA→GCA) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,093,686 | 0 | T | C | 98.2% | 181.8 / ‑4.5 | 54 | V130A (GTA→GCA) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (27/26); minor base G (1/0); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGT > AP009048/1093622‑1093751 | cAAAATGACATTGCAATATATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGt < 1:2562907/71‑1 (MQ=255) aaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGaa > 1:4270430/1‑71 (MQ=255) aTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAAt < 1:3226353/71‑1 (MQ=255) tGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGAATATTAATATTTCTGGCATTGGTGAAt > 1:5052623/1‑70 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg > 1:4957723/1‑71 (MQ=255) cAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg < 1:1778820/70‑1 (MQ=255) aaTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTg < 1:160673/71‑1 (MQ=255) tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:4656759/1‑71 (MQ=255) attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg > 1:1085215/1‑51 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:3924639/67‑1 (MQ=255) tattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:520061/1‑58 (MQ=255) attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAacac > 1:4709542/1‑70 (MQ=255) tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:3847830/1‑71 (MQ=255) tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:291230/1‑71 (MQ=255) tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaa > 1:2159413/1‑70 (MQ=255) gTTTCGTCAGATGAGTTTGTTCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa < 1:1752324/70‑1 (MQ=255) tttCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:2817519/54‑1 (MQ=255) ttCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt > 1:2085144/1‑40 (MQ=255) cGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg > 1:993245/1‑71 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCAt < 1:4236818/36‑1 (MQ=255) tCAGATGAGTTTGTGCATATTAATATTTCTGGCAt < 1:2388543/35‑1 (MQ=255) tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAAt < 1:831548/43‑1 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCaa < 1:4948783/70‑1 (MQ=255) gATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGaa > 1:3868517/1‑39 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAAc < 1:449576/61‑1 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt < 1:519367/71‑1 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatt > 1:638651/1‑70 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTa > 1:3516019/1‑45 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTa > 1:2260036/1‑45 (MQ=255) aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttat < 1:854010/70‑1 (MQ=255) aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttat < 1:2035682/70‑1 (MQ=255) gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt < 1:4711518/71‑1 (MQ=255) gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt < 1:452290/71‑1 (MQ=255) tttGTGCATATTAATATTTCTGGCATTGGTGAATaa < 1:57838/36‑1 (MQ=255) tttGTGCATATTAATATTTCTGGCATTGGTGAATaa < 1:4301126/36‑1 (MQ=255) tttGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTAtttt < 1:1583859/71‑1 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGt > 1:2722069/1‑41 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGt > 1:55268/1‑41 (MQ=255) gCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:2474632/41‑1 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAg > 1:4280540/1‑35 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaa > 1:4777892/1‑70 (MQ=255) tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa > 1:910976/1‑70 (MQ=255) tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa > 1:394261/1‑70 (MQ=255) atTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACa < 1:820434/48‑1 (MQ=255) atTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaaa > 1:443903/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt < 1:4156007/71‑1 (MQ=255) tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaa < 1:4059643/65‑1 (MQ=255) aaTATTTCTGGGATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGa > 1:4917119/1‑71 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGa > 1:2590203/1‑71 (MQ=255) atatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGa < 1:1565817/70‑1 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaa > 1:152654/1‑38 (MQ=255) tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTAtttt > 1:4572295/1‑53 (MQ=255) tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgt > 1:3986621/1‑71 (MQ=255) cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgt < 1:5092300/70‑1 (MQ=255) | CAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGT > AP009048/1093622‑1093751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |