Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,684,624 C→A 100% pseudogene (435/582 nt)
pseudogene (1498/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,684,6240CA100.0% 227.3 / NA 76pseudogene (435/582 nt)
pseudogene (1498/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/43);  total (33/43)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGACATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATGTGTTGGCCATGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGT  >  AP009048/3684557‑3684689
                                                                   |                                                                 
agaCATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACgg                                                                <  1:1189714/71‑1 (MQ=255)
 gaCATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACggg                                                               <  1:2354285/71‑1 (MQ=255)
  aCATGAAGGAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGt                                                              <  1:920665/71‑1 (MQ=255)
    aTGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGa                                                            <  1:4259043/71‑1 (MQ=255)
     tGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACTGGTGAt                                                           <  1:671207/71‑1 (MQ=255)
        aGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgtg                                                        <  1:3507937/71‑1 (MQ=255)
        aGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtgtg                                                        <  1:5165013/71‑1 (MQ=255)
             gTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtg                                                          <  1:2106536/64‑1 (MQ=255)
             gTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTgg                                                    >  1:399862/1‑70 (MQ=255)
              tATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGcc                                                  <  1:4839182/71‑1 (MQ=255)
               aTGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGc                                                   >  1:2177050/1‑69 (MQ=255)
               aTGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGc                                                   >  1:4691307/1‑69 (MQ=255)
               aTGGCCAATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGc                                                   >  1:2215158/1‑69 (MQ=255)
                tGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCag                                                >  1:1732144/1‑70 (MQ=255)
                  gCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATgtgt                                                       <  1:1858388/62‑1 (MQ=255)
                  gCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATgtgt                                                       <  1:1515511/62‑1 (MQ=255)
                  gCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCagg                                               <  1:182365/70‑3 (MQ=255)
                     cATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATg                                           >  1:3099583/1‑71 (MQ=255)
                      aTCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGc                                          >  1:2328305/1‑71 (MQ=255)
                         aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACg                                                                 >  1:2237878/1‑45 (MQ=255)
                         aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAtg                                                          >  1:2066142/1‑52 (MQ=255)
                         aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCg                                        <  1:4658705/70‑1 (MQ=255)
                         aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGa                                       <  1:1883795/71‑1 (MQ=255)
                         aCTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGa                                       <  1:3299740/71‑1 (MQ=255)
                           tGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAg                                     >  1:1833698/1‑71 (MQ=255)
                            gTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCagg                                               <  1:3832961/60‑3 (MQ=255)
                             ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGa                                       <  1:2154090/67‑1 (MQ=255)
                             ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAg                                     <  1:1764485/69‑1 (MQ=255)
                             ttCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGgc                                   >  1:2592186/1‑71 (MQ=255)
                               cAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGc                                          <  1:347510/62‑1 (MQ=255)
                               cAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGc                                          <  1:1116656/62‑1 (MQ=255)
                               cAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGgcg                                  <  1:4004335/70‑1 (MQ=255)
                                aGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACTGGTGATGTGTTGGCCAGGGATGCCGAAGgcgc                                 <  1:3591970/70‑1 (MQ=255)
                                aGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGgcgc                                 <  1:657179/70‑1 (MQ=255)
                                aGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGgcgc                                 <  1:923948/70‑1 (MQ=255)
                                aGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGgcgc                                 <  1:4066438/70‑1 (MQ=255)
                                  cATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTgg                              >  1:5123017/1‑71 (MQ=255)
                                    tGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGAt                                                           >  1:292197/1‑40 (MQ=255)
                                    tGGTGTCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGcc                                         <  1:980254/58‑1 (MQ=255)
                                      gtgtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTa                           <  1:2792776/70‑1 (MQ=255)
                                       tgtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAggg                                              <  1:3262950/50‑1 (MQ=255)
                                       tgtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAggg                                              <  1:1979443/50‑1 (MQ=255)
                                        gtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGa                                             >  1:5099123/1‑50 (MQ=255)
                                        gtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGa                                       >  1:4559772/1‑56 (MQ=255)
                                        gtCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACa                        >  1:2158710/1‑71 (MQ=255)
                                         tCGAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACa                        <  1:1057527/70‑1 (MQ=255)
                                           gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGcc                                         <  1:2099235/51‑1 (MQ=255)
                                           gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACAt                       >  1:1079959/1‑69 (MQ=255)
                                           gAGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGa                     <  1:4708252/71‑1 (MQ=255)
                                            aGTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCa                                                 <  1:2803851/42‑1 (MQ=255)
                                             gTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGa                                             >  1:2590449/1‑45 (MQ=255)
                                             gTGCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTaa                          <  1:1124486/64‑1 (MQ=255)
                                               gCCGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGa                  >  1:3751212/1‑70 (MQ=255)
                                                ccGCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGaaa                >  1:1121574/1‑71 (MQ=255)
                                                  gCTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATc              >  1:2766156/1‑71 (MQ=255)
                                                   cTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGa                     <  1:4965546/63‑1 (MQ=255)
                                                   cTTCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCg             >  1:4698713/1‑71 (MQ=255)
                                                     tCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCggg           >  1:117065/1‑71 (MQ=255)
                                                     tCAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCggg           >  1:904180/1‑71 (MQ=255)
                                                      cAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGa                     <  1:4290874/60‑1 (MQ=255)
                                                      cAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGaaa                >  1:2207063/1‑65 (MQ=255)
                                                      cAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCgggg          >  1:3835310/1‑71 (MQ=255)
                                                      cAACCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCgg            <  1:1959790/69‑1 (MQ=255)
                                                       aaCCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATAGGGGt         >  1:1344651/1‑71 (MQ=255)
                                                        aCCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAg                                     >  1:1804985/1‑42 (MQ=255)
                                                        aCCTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGt         >  1:4057112/1‑70 (MQ=255)
                                                         ccTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAg                                     >  1:3335948/1‑41 (MQ=255)
                                                          cTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGa                     >  1:4190002/1‑56 (MQ=255)
                                                          cTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTAt      <  1:574058/71‑1 (MQ=255)
                                                          cTGGTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTAt      >  1:4215796/1‑71 (MQ=255)
                                                             gTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTa                           <  1:2892502/47‑1 (MQ=255)
                                                             gTCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATcg    <  1:3173875/70‑2 (MQ=255)
                                                              tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATg                      <  1:3187533/51‑1 (MQ=39)
                                                              tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATcgg   <  1:2701113/70‑3 (MQ=255)
                                                              tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATcgg   >  1:869517/1‑68 (MQ=255)
                                                              tCTTTACGGGTGATGTGTTGGCCAGGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCGGt  <  1:5189142/71‑1 (MQ=255)
                                                                   |                                                                 
AGACATGAAGCAGGTATGGCCCATCACTGTTCAGCATGGTGTCGAGTGCCGCTTCAACCTGGTCTTTCCGGGTGATGTGTTGGCCATGGATGCCGAAGGCGCTGGCTAACATGAGGAAATCGGGGTTATCAGT  >  AP009048/3684557‑3684689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: