Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,685,267 C→T 100% pseudogene (855/1645 nt)
pseudogene (855/984 nt)
ilvG ←
ilvG ←
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,685,2670CT100.0% 236.3 / NA 79pseudogene (855/1645 nt)
pseudogene (855/984 nt)
ilvG
ilvG
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/46);  total (33/46)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACATGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCA  >  AP009048/3685199‑3685333
                                                                    |                                                                  
acaTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGgtgc                                                                  <  1:2264636/71‑1 (MQ=255)
  aTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                 >  1:2138987/1‑70 (MQ=255)
   tgtgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                 >  1:4713121/1‑69 (MQ=255)
   tgtgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                 >  1:1288722/1‑69 (MQ=255)
     tgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAg                                                              <  1:1893178/70‑1 (MQ=255)
        cTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgttt                                                          >  1:877344/1‑69 (MQ=255)
        cTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtgt                                                          >  1:812511/1‑71 (MQ=255)
        cTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtgt                                                          <  1:4747874/71‑1 (MQ=255)
           aCGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                         <  1:4952201/69‑1 (MQ=255)
           aCGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                         >  1:3476189/1‑69 (MQ=255)
           aCGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                         <  1:3463776/69‑1 (MQ=255)
           aCGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                         >  1:3259141/1‑69 (MQ=255)
           aCGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGtt                                                         <  1:176890/69‑1 (MQ=255)
            cGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAg                                                      <  1:313534/71‑1 (MQ=255)
             gCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAg                                                      <  1:3997609/70‑1 (MQ=255)
               aGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGaa                                                               <  1:1018221/59‑1 (MQ=255)
               aGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                   <  1:3986248/71‑1 (MQ=255)
                gCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                 <  1:574560/56‑1 (MQ=255)
                gCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTg                                                  <  1:845814/71‑1 (MQ=255)
                   tGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCa                                                       <  1:855847/63‑1 (MQ=255)
                   tGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGcc                                                >  1:368889/1‑70 (MQ=255)
                   tGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGcc                                                >  1:3537678/1‑70 (MQ=255)
                    gTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCgg                                              >  1:865036/1‑71 (MQ=255)
                     ttCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                     <  1:382566/63‑1 (MQ=255)
                     ttCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGt                                             <  1:1959081/71‑1 (MQ=255)
                       cATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                           <  1:2385084/55‑1 (MQ=255)
                       cATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                           <  1:1485443/55‑1 (MQ=255)
                       cATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTc                                            <  1:668379/70‑1 (MQ=255)
                       cATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCa                                           >  1:1803941/1‑71 (MQ=255)
                        aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                     <  1:2968884/60‑1 (MQ=255)
                        aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                          >  1:5093233/1‑71 (MQ=255)
                         tttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGa                                                                <  1:4735470/48‑1 (MQ=255)
                         tttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                     <  1:4918453/59‑1 (MQ=255)
                         tttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAcc                                         <  1:1267516/71‑1 (MQ=255)
                          ttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                 <  1:4422988/46‑1 (MQ=255)
                          ttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAccc                                        >  1:3296692/1‑71 (MQ=255)
                          ttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAccc                                        >  1:5021034/1‑71 (MQ=255)
                          ttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAccc                                        >  1:808109/1‑71 (MQ=255)
                            cTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGt                                             >  1:2054065/1‑64 (MQ=255)
                             tGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGt                                     >  1:3183546/1‑71 (MQ=255)
                               ccGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtca                                   >  1:3498800/1‑71 (MQ=255)
                               ccGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGt                                     <  1:3278836/69‑1 (MQ=255)
                                 gtgTCGATATCCATATGGATAACACTGGTGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcatc                                 <  1:2828383/69‑1 (MQ=255)
                                 gggTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGcc                                                >  1:3202400/1‑56 (MQ=255)
                                 gggTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcat                                  >  1:1017991/1‑70 (MQ=255)
                                 gggTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcat                                  >  1:3721445/1‑70 (MQ=255)
                                    tCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                              <  1:720660/71‑1 (MQ=255)
                                    tCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                              <  1:401431/71‑1 (MQ=255)
                                     cGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaaa                             >  1:4515838/1‑71 (MQ=255)
                                      gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                           <  1:840795/40‑1 (MQ=255)
                                       atatCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                           <  1:2446814/39‑1 (MQ=255)
                                       atatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                              <  1:1325372/68‑1 (MQ=255)
                                       atatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACg                           <  1:4201311/71‑1 (MQ=255)
                                        tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGtt                                                    >  1:4963693/1‑45 (MQ=255)
                                        tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGt                          <  1:4461339/71‑1 (MQ=255)
                                         atCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                               <  1:1047269/65‑1 (MQ=255)
                                           ccATATGGATAACACTGGCGTGTGGTGCGAAGgtgt                                                          <  1:4049287/36‑1 (MQ=255)
                                            cATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTgcac                      >  1:484074/1‑69 (MQ=255)
                                            cATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTgca                       <  1:36177/70‑2 (MQ=255)
                                             atatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCg                                               >  1:5180397/1‑45 (MQ=255)
                                             atatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCg                                               >  1:16283/1‑45 (MQ=255)
                                             atatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCa                                           <  1:604654/49‑1 (MQ=255)
                                             atatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                               <  1:2897105/61‑1 (MQ=255)
                                              tatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGt                                             <  1:3688407/46‑1 (MQ=255)
                                              tatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCAcc                     <  1:3213817/70‑1 (MQ=255)
                                                tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCAc                  <  1:3936508/71‑1 (MQ=255)
                                                  gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                               <  1:2504746/56‑1 (MQ=255)
                                                  gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGt                          >  1:1260738/1‑61 (MQ=255)
                                                  gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACgg                >  1:1342044/1‑71 (MQ=255)
                                                  gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACgg                <  1:758020/71‑1 (MQ=255)
                                                  gATAACACTGGCGTGTGGTGCGAAGCTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGt                          >  1:3671984/1‑61 (MQ=255)
                                                    tAACACTGGCGTGCGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGc               >  1:1260536/1‑70 (MQ=38)
                                                      acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt            >  1:4560832/1‑71 (MQ=255)
                                                      acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt            <  1:2740506/71‑1 (MQ=255)
                                                       cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                          >  1:2571583/1‑40 (MQ=255)
                                                       cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                          >  1:4607277/1‑40 (MQ=255)
                                                          tGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAccc                                        >  1:495867/1‑39 (MQ=255)
                                                            gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa      <  1:5163421/71‑2 (MQ=255)
                                                            gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa      <  1:3374351/71‑2 (MQ=255)
                                                            gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc        >  1:4262953/1‑69 (MQ=255)
                                                            gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATca          <  1:4016201/67‑1 (MQ=255)
                                                              gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt    >  1:1827315/1‑71 (MQ=39)
                                                              gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt    <  1:338256/71‑1 (MQ=39)
                                                                 tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaag     <  1:2695586/67‑3 (MQ=38)
                                                                 tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg  <  1:4942132/70‑2 (MQ=38)
                                                                    |                                                                  
ACATGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCA  >  AP009048/3685199‑3685333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: