| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,329 | G→A | 100% | pseudogene (793/1645 nt) pseudogene (793/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,329 | 0 | G | A | 100.0% | 228.1 / NA | 76 | pseudogene (793/1645 nt) pseudogene (793/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (37/39); total (37/39) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGT > AP009048/3685261‑3685394 | gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt > 1:1827315/1‑71 (MQ=39)gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:338256/71‑1 (MQ=39) tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg < 1:4942132/70‑2 (MQ=38) gtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac < 1:1200475/69‑1 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt < 1:4747600/70‑1 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt > 1:1801817/2‑71 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt > 1:3990593/2‑71 (MQ=38) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc > 1:4867980/1‑71 (MQ=39) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc > 1:3247028/1‑71 (MQ=39) gAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac < 1:1544752/67‑1 (MQ=38) gAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCt < 1:247211/71‑1 (MQ=39) aGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc < 1:4383247/71‑1 (MQ=39) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAc < 1:4641247/71‑1 (MQ=39) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg < 1:1220646/70‑1 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:791781/1‑70 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg < 1:1974466/70‑1 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:1756024/1‑69 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGa < 1:4689141/70‑1 (MQ=38) tttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:3182603/71‑1 (MQ=39) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:3886749/1‑70 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:4638580/70‑3 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:2791040/70‑3 (MQ=255) ggTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGaa > 1:4101647/1‑63 (MQ=38) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:876138/1‑71 (MQ=255) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:1369833/1‑71 (MQ=255) tCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt < 1:4487747/71‑1 (MQ=255) tCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt > 1:2917296/1‑71 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAGGTTCGCCGCtt > 1:4715110/1‑71 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt > 1:4754213/1‑71 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg < 1:2238785/42‑2 (MQ=25) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt > 1:4617108/1‑71 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:1541728/71‑1 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:1056266/70‑1 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:5080745/1‑71 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:2770940/1‑71 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:4355081/71‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTgg > 1:3243188/1‑71 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc > 1:501349/1‑70 (MQ=255) atcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGccat < 1:1502340/71‑3 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:3298459/1‑52 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAc < 1:5007782/42‑1 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:2787486/1‑61 (MQ=255) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAt > 1:1705933/1‑71 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGa > 1:4284679/1‑45 (MQ=38) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:1055331/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:698636/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:1081978/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:4765006/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:1968242/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:2551105/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTCGGTGCCATGCAtt > 1:710355/1‑70 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4683121/1‑69 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4676943/1‑69 (MQ=255) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCCCTTTGGTGCCATGCATtcc < 1:4503563/71‑1 (MQ=255) gTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc > 1:505713/1‑70 (MQ=255) gcacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:4820374/4‑71 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:755484/68‑1 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGc < 1:21583/69‑1 (MQ=255) acccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:2307250/59‑1 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:1571955/1‑71 (MQ=255) cACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGccat < 1:1734351/56‑3 (MQ=255) cACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGc > 1:3002754/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:2192827/1‑71 (MQ=255) cGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGccc < 1:115780/71‑1 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:1196409/1‑69 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:5166988/71‑1 (MQ=38) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:2342676/41‑1 (MQ=25) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcc < 1:2323337/47‑1 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc < 1:2193844/55‑3 (MQ=255) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:4631419/54‑2 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCa < 1:721863/58‑1 (MQ=255) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:3926905/67‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcc < 1:4914585/46‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGAt < 1:5100656/69‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:3304586/1‑71 (MQ=37) tCAGCAAGTCGCACTCCTGCACAGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:2918672/1‑71 (MQ=37) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:2956891/1‑40 (MQ=25) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGt > 1:3275038/1‑42 (MQ=37) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc > 1:1429166/1‑55 (MQ=255) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:2394940/1‑70 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:1510775/56‑1 (MQ=255) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:1172998/1‑60 (MQ=255) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGt < 1:3702343/70‑1 (MQ=37) | GTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGT > AP009048/3685261‑3685394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |