| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,369 | G→A | 100% | pseudogene (753/1645 nt) pseudogene (753/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,369 | 0 | G | A | 100.0% | 242.5 / NA | 82 | pseudogene (753/1645 nt) pseudogene (753/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (45/37); total (45/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGC > AP009048/3685304‑3685432 | aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAt > 1:1705933/1‑71 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:1055331/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:1081978/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:698636/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:1968242/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:2551105/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:4765006/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTCGGTGCCATGCAtt > 1:710355/1‑70 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4683121/1‑69 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4676943/1‑69 (MQ=255) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCCCTTTGGTGCCATGCATtcc < 1:4503563/71‑1 (MQ=255) gTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc > 1:505713/1‑70 (MQ=255) gcacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:4820374/4‑71 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:755484/68‑1 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGc < 1:21583/69‑1 (MQ=255) acccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:2307250/59‑1 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:1571955/1‑71 (MQ=255) cACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGc > 1:3002754/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:2192827/1‑71 (MQ=255) cGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGccc < 1:115780/71‑1 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:1196409/1‑69 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:5166988/71‑1 (MQ=38) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc < 1:2193844/55‑3 (MQ=255) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:4631419/54‑2 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCa < 1:721863/58‑1 (MQ=255) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:3926905/67‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGAt < 1:5100656/69‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:3304586/1‑71 (MQ=37) tCAGCAAGTCGCACTCCTGCACAGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:2918672/1‑71 (MQ=37) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc > 1:1429166/1‑55 (MQ=255) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:2394940/1‑70 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg < 1:1510775/56‑1 (MQ=255) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:1172998/1‑60 (MQ=255) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGt < 1:3702343/70‑1 (MQ=37) aagTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACgg < 1:3037804/69‑1 (MQ=38) agTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa > 1:3279343/2‑71 (MQ=38) tcgcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGAt > 1:3010994/4‑70 (MQ=38) cgcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGt > 1:1646523/3‑63 (MQ=37) cacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATc > 1:2984215/1‑71 (MQ=39) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCa > 1:3497100/1‑48 (MQ=37) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCa > 1:3359547/1‑48 (MQ=37) cTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCt > 1:1371913/1‑70 (MQ=38) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc > 1:4467631/1‑71 (MQ=39) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAACCt < 1:2219940/69‑1 (MQ=38) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc < 1:2900890/69‑1 (MQ=38) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt > 1:1542386/1‑71 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt < 1:1809597/71‑1 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt > 1:1435541/1‑71 (MQ=39) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:553896/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:2246528/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:4411536/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:3093070/1‑71 (MQ=39) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa < 1:1105800/71‑1 (MQ=39) cgcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCt < 1:4352607/67‑1 (MQ=38) gcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg < 1:3591236/48‑1 (MQ=25) gcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACt > 1:3161080/1‑69 (MQ=38) gcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTg > 1:939176/1‑70 (MQ=38) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa > 1:3035855/1‑49 (MQ=25) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa > 1:2085669/1‑49 (MQ=25) aaGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGcc < 1:1505765/71‑1 (MQ=39) aaGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGcc > 1:2611034/1‑71 (MQ=39) gTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt < 1:5085663/53‑1 (MQ=37) gTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:2070717/1‑62 (MQ=38) gTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGcc > 1:3959568/1‑69 (MQ=38) gTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCa < 1:2184381/71‑1 (MQ=39) ttcgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCa < 1:836359/67‑1 (MQ=38) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCa < 1:627908/67‑1 (MQ=39) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:103351/2‑71 (MQ=255) ccGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCt < 1:1468132/71‑1 (MQ=255) ccGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCt > 1:3318768/1‑71 (MQ=255) cGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGc > 1:1941287/1‑67 (MQ=255) cGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCtt < 1:2717297/71‑1 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTAc > 1:194704/1‑56 (MQ=38) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt < 1:1226482/71‑1 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCtt > 1:4046486/1‑70 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGCATAATCTGCTTCTACTGCGCCCAGCCCttt < 1:4503204/71‑1 (MQ=39) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt > 1:1227781/1‑70 (MQ=255) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt > 1:3648965/1‑70 (MQ=255) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCtt > 1:4693898/1‑69 (MQ=255) ttGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:2000505/1‑52 (MQ=37) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAg < 1:1303935/70‑1 (MQ=255) ggTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGc < 1:3765301/70‑1 (MQ=255) | AAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGC > AP009048/3685304‑3685432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |