| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 2,809,581 | G→A | 45.0% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 2,809,581 | 0 | G | A | 45.0% | 51.9 / 174.5 | 129 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (30/41); new base A (22/36); total (52/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCT > AP009048/2809514‑2809650 | cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt < 1:1820980/71‑1 (MQ=255) tCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt > 1:1088827/1‑70 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa < 1:5310981/70‑1 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc < 1:2735286/71‑1 (MQ=255) cTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:2888758/1‑69 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:4897229/1‑68 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgaga < 1:1579508/70‑1 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgag < 1:1251047/69‑1 (MQ=255) tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttg > 1:4851397/1‑71 (MQ=255) tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttg > 1:2822300/1‑71 (MQ=255) gtgcatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt < 1:4181090/65‑1 (MQ=255) gtgcatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt < 1:3986476/65‑1 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:3194843/1‑71 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt < 1:3251972/69‑1 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt < 1:3708117/70‑1 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt < 1:2272291/70‑1 (MQ=255) gcatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg > 1:1882089/2‑68 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:5318669/71‑1 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:1104651/71‑1 (MQ=255) catgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa < 1:1499967/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa < 1:3325709/52‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga > 1:3996436/1‑61 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt < 1:3289204/70‑1 (MQ=255) gcaAAGCAATCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg > 1:4114780/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:1232540/1‑71 (MQ=255) aaaGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg > 1:3810863/1‑71 (MQ=255) aaaGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCg < 1:5348829/71‑1 (MQ=255) aagcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCg > 1:1309038/1‑70 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag < 1:1353721/54‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg < 1:1602053/71‑1 (MQ=255) caagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt < 1:3004757/57‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:467898/45‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc < 1:1130947/46‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:3460134/57‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:537169/43‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa < 1:3609686/71‑1 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg > 1:3974433/1‑61 (MQ=255) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc > 1:3787688/1‑70 (MQ=255) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:2924430/64‑1 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa > 1:474625/1‑41 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:3759093/52‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt > 1:4083656/1‑53 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTg < 1:5174205/62‑1 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:3193288/1‑70 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:4746230/1‑70 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:93606/1‑70 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:2810953/1‑70 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:5118424/1‑53 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa < 1:1769865/71‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:2608746/69‑1 (MQ=255) agaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg < 1:4870941/71‑1 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:2654201/1‑37 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc > 1:1236762/1‑71 (MQ=255) aCCGCAATATAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:1567197/71‑1 (MQ=255) aCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:4418511/71‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:5111981/71‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:2141562/71‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:2998278/71‑1 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa < 1:188881/71‑1 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt > 1:4493052/1‑52 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCg < 1:4122532/49‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:327811/68‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:561468/1‑70 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTAt < 1:3209537/43‑1 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:2880396/1‑68 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:2733729/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:4337663/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:3141976/1‑70 (MQ=255) aaTGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:5014434/36‑1 (MQ=255) aaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:2613663/67‑1 (MQ=255) aaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc > 1:1469674/1‑70 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt > 1:4693697/1‑44 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa > 1:4342801/1‑56 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGCAGTCTCAGGAAAACca < 1:3496692/71‑1 (MQ=255) tGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg > 1:3464898/1‑50 (MQ=255) tGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg > 1:2808703/1‑56 (MQ=255) tGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca > 1:909573/1‑70 (MQ=255) gCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcac > 1:1767617/1‑70 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg < 1:4405767/39‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:5288295/71‑1 (MQ=255) tGAAGGCTCAGGGGATTAACGAGACTTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca > 1:3742445/1‑67 (MQ=255) tGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:5368097/40‑1 (MQ=255) tGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:3651363/40‑1 (MQ=255) tGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTT‑ATTACGCTGGa < 1:2292535/51‑1 (MQ=255) tGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc < 1:2504529/65‑1 (MQ=255) gAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:911605/61‑1 (MQ=255) gAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTa > 1:1472053/1‑71 (MQ=255) gAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGTAGTCTCAGGAAAACCACAg < 1:4823639/69‑1 (MQ=255) gAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:1329786/66‑1 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt > 1:3618187/1‑71 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg > 1:182804/1‑58 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTa < 1:5068661/70‑1 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt < 1:5349622/71‑1 (MQ=255) gCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt > 1:181703/1‑69 (MQ=255) gCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:5288259/71‑1 (MQ=255) cTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAg < 1:3981616/71‑1 (MQ=255) tCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg < 1:1881430/46‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:1618245/50‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt < 1:1148492/67‑1 (MQ=255) aGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc < 1:2049622/46‑1 (MQ=255) | CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCT > AP009048/2809514‑2809650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |