| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,513 | G→A | 100% | pseudogene (546/582 nt) pseudogene (1609/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,513 | 0 | G | A | 100.0% | 271.3 / NA | 94 | pseudogene (546/582 nt) pseudogene (1609/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (48/46); total (48/46) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCCC > AP009048/3684446‑3684578 | aaGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCAcc < 1:166292/70‑1 (MQ=255)aaGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCa < 1:4088926/71‑1 (MQ=255) aGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAg < 1:2726649/71‑1 (MQ=255) gCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAgg > 1:4174078/1‑71 (MQ=255) cGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggc < 1:1898357/71‑1 (MQ=255) gAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCa > 1:1108349/1‑67 (MQ=255) gAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcg < 1:4298700/71‑1 (MQ=255) gAGCCGATACATTGACCTGATGTTGCATCATCATAATTTCTCCAACATTTCTGAATTACTGGCACCa > 1:2052313/1‑67 (MQ=255) ccGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCa > 1:3824809/1‑71 (MQ=255) ccGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCa > 1:3848250/1‑71 (MQ=255) cGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc < 1:5123972/71‑1 (MQ=255) aTACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCa < 1:760618/71‑1 (MQ=255) tACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAg < 1:4280959/71‑1 (MQ=255) aCATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc > 1:2747714/1‑71 (MQ=255) cATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCAcc > 1:2553314/1‑57 (MQ=255) aTTGACCTGATGTTGCATCATGATAATTTCTCCGACATTTCTGAATTACTGGCACCAGGCGGCACCAGCgg < 1:4720108/71‑1 (MQ=255) aTTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCgg > 1:3388644/1‑71 (MQ=255) ttGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCg > 1:4383331/1‑69 (MQ=255) ttGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc < 1:4622489/71‑1 (MQ=255) tGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:4616240/1‑70 (MQ=255) gACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:3217824/1‑69 (MQ=255) gACCTGATGTTGCATCAAGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGcc < 1:720566/70‑1 (MQ=255) tGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc > 1:4945662/1‑62 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACg < 1:973240/69‑1 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGtt > 1:1638101/1‑71 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGtt > 1:5008668/1‑71 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGtt > 1:526907/1‑71 (MQ=255) gTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtct > 1:1289337/1‑71 (MQ=255) gTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGAGGCCAGACGTtct > 1:3203522/1‑71 (MQ=255) tGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGAc > 1:1554972/1‑64 (MQ=255) gCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCa < 1:2742318/70‑1 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtct < 1:2572359/66‑1 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt > 1:4202784/1‑71 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt < 1:3643132/71‑1 (MQ=255) catGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCa < 1:4216579/56‑1 (MQ=255) catGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc > 1:21204/1‑71 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcg > 1:728031/1‑42 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCa > 1:2394078/1‑55 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc < 1:33435/70‑1 (MQ=255) gATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcggc > 1:2713826/1‑42 (MQ=255) aTAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCaa > 1:4902561/1‑63 (MQ=255) aTAATTTCTCCAACATTTCTGAATTACTGGCAACAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtc < 1:1075011/70‑1 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtcg < 1:2014366/70‑1 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtcg < 1:3746463/70‑1 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtc > 1:2726145/1‑69 (MQ=255) tAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGa < 1:1504642/71‑1 (MQ=255) aTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:3007744/71‑1 (MQ=255) aTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAt < 1:2359427/70‑1 (MQ=255) tttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTg < 1:799649/71‑1 (MQ=255) tttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTg < 1:4626188/71‑1 (MQ=255) ttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAg > 1:3449118/1‑59 (MQ=255) ttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:390594/1‑69 (MQ=255) tctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:5255861/1‑68 (MQ=255) ctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTgag < 1:641831/70‑1 (MQ=255) tcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:2778190/66‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCg > 1:294945/1‑38 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACa < 1:692694/70‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACa < 1:1093006/70‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt < 1:2747933/71‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt > 1:4744906/1‑71 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGAc > 1:4746390/1‑44 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACg > 1:4281667/1‑45 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACg > 1:5123396/1‑45 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGtt > 1:3966431/1‑55 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg < 1:864307/71‑1 (MQ=255) aaCATTTCTGAATTACTGACACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg < 1:2570648/71‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAt > 1:891656/1‑61 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg > 1:2803017/1‑70 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg > 1:2580296/1‑70 (MQ=255) cATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTTAAGTTCGTCGATTGAGACATg > 1:4847126/1‑69 (MQ=255) cATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGaa < 1:3574195/71‑1 (MQ=255) cATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGaa < 1:632175/71‑1 (MQ=255) aTTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAg < 1:1977764/71‑1 (MQ=255) aTTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAg < 1:2174050/71‑1 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACa < 1:4720616/65‑1 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAg > 1:2939701/1‑70 (MQ=255) ttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc > 1:5047311/1‑51 (MQ=255) cTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtc > 1:2091296/1‑52 (MQ=255) cTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAgg > 1:4736899/1‑71 (MQ=255) tGAATTACTGCCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTga < 1:2580647/57‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGa < 1:1512065/51‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAgg < 1:5105156/68‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGt < 1:762366/69‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTa < 1:4826001/70‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTAt < 1:4602848/71‑1 (MQ=255) aTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATg > 1:2708607/1‑71 (MQ=255) ttACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATgg > 1:1974707/1‑71 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc > 1:1071488/1‑36 (MQ=255) aCTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgtc > 1:3033486/1‑45 (MQ=255) cTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt > 1:5155613/1‑39 (MQ=255) cTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTgaga < 1:1323622/52‑1 (MQ=255) cTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGAc > 1:1066104/1‑53 (MQ=255) cTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGccc < 1:484074/71‑1 (MQ=255) tGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGc < 1:3573719/59‑1 (MQ=255) | AAGCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCCC > AP009048/3684446‑3684578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |