Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,685,038 C→T 100% pseudogene (21/582 nt)
pseudogene (1084/1645 nt)
ilvG ←
ilvG ←
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,685,0380CT100.0% 333.5 / NA 109pseudogene (21/582 nt)
pseudogene (1084/1645 nt)
ilvG
ilvG
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (58/51);  total (58/51)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 109 total aligned reads displayed.

CGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG  >  AP009048/3684975‑3685103
                                                               |                                                                 
cgcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgt                                                             <  1:1889564/70‑1 (MQ=255)
cgcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAg                                                              <  1:2738153/69‑1 (MQ=255)
 gcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           >  1:4245029/1‑71 (MQ=255)
 gcAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAg                                                              >  1:2460756/1‑68 (MQ=255)
  cAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttgt                                                          <  1:154600/71‑1 (MQ=255)
    gCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGttt                                                        <  1:1013908/71‑1 (MQ=255)
     cccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       <  1:3115682/71‑1 (MQ=255)
      ccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      <  1:2352833/71‑1 (MQ=255)
      ccACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      <  1:3072017/71‑1 (MQ=255)
       cacaTCTGGTGCTGCCCCACATGTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:1396070/1‑71 (MQ=255)
       cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           <  1:5309893/65‑1 (MQ=255)
       cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:476724/1‑71 (MQ=255)
       cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:2168169/1‑71 (MQ=255)
       cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:2870025/1‑71 (MQ=255)
       cacaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTa                                                      >  1:2148169/1‑70 (MQ=255)
        acaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgtt                                                            >  1:1074976/1‑63 (MQ=255)
        acaTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaac                                                    <  1:2151114/71‑1 (MQ=255)
         caTCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaaca                                                   <  1:4772658/71‑1 (MQ=255)
           tCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:1396156/1‑70 (MQ=255)
            cTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           <  1:3268338/60‑1 (MQ=255)
            cTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 >  1:4795144/1‑70 (MQ=255)
              ggTGCTGCCACACATCTCTGGTCACGACGCAATACGCAGGTTTACGATCTGACAGTTGTTTTaacaa                                                  >  1:2196862/1‑67 (MQ=38)
              cgTGCTGCCCCACATCTGTGGTCACGCCGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaac                                              >  1:887236/2‑71 (MQ=255)
               gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaaca                                                <  1:4095306/68‑1 (MQ=255)
               gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 <  1:3754583/67‑1 (MQ=255)
               gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg                                             <  1:4169181/71‑1 (MQ=255)
               gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg                                             <  1:986115/71‑1 (MQ=255)
               gTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACg                                             >  1:1934271/1‑71 (MQ=255)
                 gctgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgc                                           >  1:4071160/1‑71 (MQ=255)
                   tgcCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgcgc                                         >  1:5360397/1‑71 (MQ=255)
                    gcCCCACATCTGTGGTCACGACGCAATCTGCAGGTTTACGATCTGACAgttgtt                                                         <  1:3411967/54‑1 (MQ=39)
                     ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgtt                                                            >  1:1747217/1‑50 (MQ=255)
                     ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:3836477/1‑70 (MQ=255)
                     ccccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:1669480/1‑70 (MQ=255)
                      cccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:4537226/1‑69 (MQ=255)
                      cccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGcgcg                                        >  1:1817824/1‑69 (MQ=255)
                      cccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTa                                      <  1:4288432/71‑1 (MQ=255)
                      cccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTa                                      <  1:520808/71‑1 (MQ=255)
                       ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgttg                                                           <  1:4871085/49‑1 (MQ=255)
                       ccACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               <  1:480412/61‑1 (MQ=255)
                        cacaTCTTTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGtttt                                                       <  1:5083808/52‑1 (MQ=39)
                        cacaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    <  1:3518062/71‑1 (MQ=255)
                         acaTTTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:1013093/1‑70 (MQ=255)
                         acaTCTGTGGTTACGACGCAATCCGCAGGTTTACTATCTGACAGTTGTTTTaa                                                     >  1:3129998/1‑53 (MQ=255)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGAGAGTTGtttt                                                       >  1:3704811/1‑51 (MQ=38)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAgt                                                             >  1:1701814/1‑45 (MQ=255)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaac                                              >  1:2591500/1‑60 (MQ=255)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaac                                                 <  1:5210023/57‑1 (MQ=255)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaa                                                     >  1:2223381/1‑53 (MQ=255)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGgcgc                                         >  1:2201255/1‑65 (MQ=255)
                         acaTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGAt                                   <  1:466723/71‑1 (MQ=255)
                          caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               >  1:4311334/1‑58 (MQ=255)
                          caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATa                                  <  1:2876552/71‑1 (MQ=255)
                          caTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATa                                  <  1:2680274/71‑1 (MQ=255)
                           aTCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAg                                 >  1:487995/1‑71 (MQ=255)
                            tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGGTAGc                                >  1:366909/1‑71 (MQ=255)
                            tCTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                >  1:310519/1‑71 (MQ=255)
                            tCTGTGGTCACGACGCAATACGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAg                                 >  1:4568770/1‑70 (MQ=255)
                             cTGTGGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCg                               >  1:2696011/1‑71 (MQ=255)
                               gtgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTaacaacaa                                               <  1:511386/53‑1 (MQ=255)
                                tgGTCACGACGCAATCCGCAGGTTTATGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                <  1:781347/67‑1 (MQ=255)
                                tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGc                                <  1:3073229/67‑1 (MQ=255)
                                tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCa                            <  1:4095509/71‑1 (MQ=255)
                                tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCa                            <  1:2776129/71‑1 (MQ=255)
                                tgGTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCa                            >  1:2944050/1‑71 (MQ=255)
                                 ggTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGTCGCGTAGATAGCGt                              >  1:2364798/1‑68 (MQ=255)
                                  gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:5195863/1‑61 (MQ=255)
                                  gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc                           <  1:1460946/70‑1 (MQ=255)
                                  gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc                           <  1:898330/70‑1 (MQ=255)
                                  gTCACGACGCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCAc                           <  1:2267809/70‑1 (MQ=255)
                                     acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:4691040/1‑70 (MQ=255)
                                     acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:4729428/1‑70 (MQ=255)
                                     acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:788683/1‑70 (MQ=255)
                                     acgacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCgg                        >  1:4948025/1‑70 (MQ=255)
                                       gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    <  1:241792/56‑1 (MQ=255)
                                       gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt                     <  1:1473334/71‑1 (MQ=255)
                                       gacgCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGAt                     >  1:1213995/1‑71 (MQ=255)
                                          gCAATCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGt                  >  1:4841499/1‑71 (MQ=255)
                                            aaTCCGCAGGTTTACGATCTGACAGTTGTTTTaaca                                                   <  1:3927529/36‑1 (MQ=255)
                                             aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:616867/71‑1 (MQ=255)
                                             aTCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:2346085/71‑1 (MQ=255)
                                              tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTc                             <  1:1887514/56‑1 (MQ=255)
                                              tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               >  1:762248/1‑70 (MQ=255)
                                              tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               >  1:768545/1‑70 (MQ=255)
                                              tCCGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               >  1:5346524/1‑70 (MQ=255)
                                               ccGCAGGTTTACGATCTGACAGTTGTTTTaacaacaac                                              >  1:3937783/1‑38 (MQ=255)
                                               ccGCAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTa              >  1:3961140/1‑70 (MQ=255)
                                               ccGCAGGTTTACGATCTGACAGTTCTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTaa             >  1:5201953/1‑71 (MQ=255)
                                                  cAGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          <  1:4246432/71‑1 (MQ=255)
                                                  cAGGTTTACGATCTGACAGTTGTTTTAAAAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          >  1:5093283/1‑71 (MQ=255)
                                                   aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    <  1:2403607/44‑1 (MQ=255)
                                                   aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCg                         <  1:2406928/55‑1 (MQ=255)
                                                   aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGt               <  1:4092679/65‑1 (MQ=255)
                                                   aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGcc         <  1:637399/71‑1 (MQ=255)
                                                   aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          >  1:589771/1‑70 (MQ=255)
                                                   aGGTTTACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGc          >  1:1462365/1‑70 (MQ=255)
                                                       ttACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGa                                    >  1:5336603/1‑40 (MQ=255)
                                                        tACGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAggca    >  1:3087833/1‑69 (MQ=255)
                                                          cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCAt   <  1:987894/70‑1 (MQ=255)
                                                          cGATCTGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGCATg  >  1:3986742/1‑71 (MQ=255)
                                                               |                                                                 
CGCAGCCCACATCTGGTGCTGCCCCACATCTGTGGTCACGACGCAATCCGCAGGTTTACGATCCGACAGTTGTTTTAACAACAACGGCGCGTAGATAGCGTCACCGGGATGGTCGTAACGCCAGGAATG  >  AP009048/3684975‑3685103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: