Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 3,685,267 C→T 100% pseudogene (855/1645 nt)
pseudogene (855/984 nt)
ilvG ←
ilvG ←
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090483,685,2670CT100.0% 267.7 / NA 89pseudogene (855/1645 nt)
pseudogene (855/984 nt)
ilvG
ilvG
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II
ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/46);  total (43/46)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCAC  >  AP009048/3685200‑3685334
                                                                   |                                                                   
caTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGgtgc                                                                   >  1:1696900/1‑70 (MQ=255)
 aTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  >  1:1854917/1‑70 (MQ=255)
 aTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGa                                                                 <  1:111506/71‑1 (MQ=255)
 aTGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGa                                                                 <  1:775719/71‑1 (MQ=255)
  tgtgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  >  1:2853102/1‑69 (MQ=255)
  tgtgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGaa                                                                <  1:590916/71‑1 (MQ=255)
    tgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAgg                                                              >  1:1129928/1‑71 (MQ=255)
    tgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAgg                                                              <  1:364419/71‑1 (MQ=255)
    tgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAg                                                               <  1:2775997/70‑1 (MQ=255)
    tgCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAg                                                               <  1:4615509/70‑1 (MQ=255)
     gCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgt                                                             >  1:1819391/1‑71 (MQ=255)
      ccTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                            >  1:2331132/1‑71 (MQ=255)
           cGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAg                                                       >  1:5349523/1‑71 (MQ=255)
           cGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAg                                                       <  1:4797344/71‑1 (MQ=255)
            gCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAgg                                                              <  1:3030931/63‑1 (MQ=255)
            gCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                      >  1:2762439/1‑71 (MQ=255)
              aGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGttt                                                    <  1:5059086/71‑1 (MQ=255)
               gCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTg                                                   >  1:1187168/1‑71 (MQ=255)
               gCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTg                                                   <  1:3635875/71‑1 (MQ=255)
                cTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGc                                                  >  1:3690431/1‑71 (MQ=255)
                 ttGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  <  1:123986/54‑1 (MQ=255)
                   gTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGcc                                                 <  1:3084467/69‑1 (MQ=255)
                   gTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCgg                                               >  1:903787/1‑71 (MQ=255)
                   gTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCgg                                               >  1:3325294/1‑71 (MQ=255)
                   gTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCg                                                <  1:4212656/70‑1 (MQ=255)
                    ttCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGc                                                  <  1:842234/67‑1 (MQ=255)
                    ttCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGt                                              >  1:3812263/1‑71 (MQ=255)
                     tCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  <  1:3375244/50‑1 (MQ=255)
                     tCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGtgcg                                                                  <  1:3122049/50‑1 (MQ=255)
                       aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                           >  1:420937/1‑71 (MQ=255)
                       aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                           >  1:475581/1‑71 (MQ=255)
                       aTTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                           <  1:976495/71‑1 (MQ=255)
                        tttCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTg                                                   <  1:956242/62‑1 (MQ=255)
                          tCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGcc                                                 >  1:4787572/1‑62 (MQ=255)
                           cTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGt                                                      >  1:186973/1‑56 (MQ=255)
                           cTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCgg                                       >  1:238126/1‑71 (MQ=255)
                              cctggTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtca                                    <  1:2338314/68‑1 (MQ=255)
                              ccGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtca                                    <  1:1461979/71‑1 (MQ=255)
                              ccGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtc                                     <  1:1838560/70‑1 (MQ=255)
                              ccGGGTCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTAAGTTTGCCGGTCACCCGGtca                                    <  1:1745697/71‑1 (MQ=255)
                                   tCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAg                                                               <  1:1156750/39‑1 (MQ=255)
                                   tCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                               <  1:3668831/71‑1 (MQ=255)
                                   tCGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                               <  1:2088555/71‑1 (MQ=255)
                                    cGATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaaa                              >  1:758988/1‑71 (MQ=255)
                                     gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGgtg                                                            <  1:1531580/40‑1 (MQ=255)
                                     gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAAc                             <  1:2298464/71‑1 (MQ=255)
                                     gATATCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAAc                             <  1:1858014/71‑1 (MQ=255)
                                      atatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaa                               <  1:3071995/68‑1 (MQ=255)
                                       tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTg                                                   <  1:1061718/47‑1 (MQ=255)
                                       tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCg                                                >  1:1809232/1‑50 (MQ=255)
                                       tatCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACgg                           <  1:1632109/71‑2 (MQ=255)
                                        atCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGt                           >  1:3748172/1‑70 (MQ=255)
                                        atCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTg                          >  1:2185051/1‑71 (MQ=255)
                                        atCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTg                          >  1:391536/1‑71 (MQ=255)
                                         tCCATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTg                          <  1:2953091/70‑1 (MQ=255)
                                           cATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGt                                      >  1:5181118/1‑56 (MQ=255)
                                           cATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGt                                      >  1:3150483/1‑56 (MQ=255)
                                           cATATGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTgcac                       >  1:1380358/1‑69 (MQ=255)
                                            atatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGc                                                  <  1:791364/43‑1 (MQ=255)
                                             tatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                                <  1:4473663/60‑1 (MQ=255)
                                              atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGcc                                                 >  1:3808670/1‑42 (MQ=255)
                                              atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa                    >  1:5124625/1‑71 (MQ=255)
                                              atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa                    >  1:21752/1‑71 (MQ=255)
                                               tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCAc                   <  1:1437810/71‑1 (MQ=255)
                                                ggATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACg                            >  1:3608900/1‑61 (MQ=255)
                                                 gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtcat                                   <  1:4888677/53‑1 (MQ=255)
                                                 gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaa                                <  1:451521/56‑1 (MQ=255)
                                                 gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAAc                             <  1:5233466/59‑1 (MQ=255)
                                                  aTAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCaaaa                              >  1:1636940/1‑57 (MQ=255)
                                                   tAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGt                           >  1:301209/1‑59 (MQ=255)
                                                   tAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCg               <  1:4394876/71‑1 (MQ=255)
                                                    aaCACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGa              >  1:4887859/1‑71 (MQ=255)
                                                    aaCACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGACGGTCACCCGGtca                                    <  1:443374/49‑1 (MQ=255)
                                                     acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             <  1:3593664/71‑1 (MQ=255)
                                                     acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             <  1:3107815/71‑1 (MQ=255)
                                                     acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             >  1:3971412/1‑71 (MQ=255)
                                                     acacTGGCGTGTGGTGCGAAGGTGTTCAGTCTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGa              <  1:864265/70‑1 (MQ=255)
                                                      cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCAc                                           >  1:4512031/1‑40 (MQ=255)
                                                       acTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGt                                              >  1:4916855/1‑36 (MQ=255)
                                                       acTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt             >  1:868972/1‑69 (MQ=255)
                                                        cTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATca           >  1:3264594/1‑70 (MQ=255)
                                                         tGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGtc                                     >  1:3871555/1‑43 (MQ=255)
                                                         tGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTgca                        >  1:2439248/1‑55 (MQ=255)
                                                         tGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcag          >  1:3307868/1‑70 (MQ=255)
                                                         tGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcag          >  1:1112109/1‑70 (MQ=255)
                                                          ggCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         >  1:4229592/1‑70 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:3596526/69‑1 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         >  1:1414831/1‑69 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:5220803/69‑1 (MQ=255)
                                                           gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:314836/69‑1 (MQ=255)
                                                           gCGTGTGATGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc         <  1:4408162/69‑1 (MQ=38)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcgc  <  1:4682490/71‑3 (MQ=38)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg   <  1:1184269/70‑2 (MQ=38)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg   >  1:2258929/1‑69 (MQ=38)
                                                                tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg   >  1:1820095/1‑69 (MQ=38)
                                                                   |                                                                   
CATGTGCCTGACGCAGCTTGTTCATTTCTGCCGGGTCGATATCCATATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCAC  >  AP009048/3685200‑3685334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: