| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,369 | G→A | 100% | pseudogene (753/1645 nt) pseudogene (753/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,369 | 0 | G | A | 100.0% | 246.4 / NA | 84 | pseudogene (753/1645 nt) pseudogene (753/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (47/37); total (47/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTA > AP009048/3685301‑3685435 | tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:2266911/71‑1 (MQ=255)tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:1234739/71‑1 (MQ=255)tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:5362838/71‑1 (MQ=255)tcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:991824/71‑1 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg > 1:4125779/1‑70 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGc < 1:3078382/70‑1 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCa > 1:3207665/1‑71 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCa > 1:4756124/1‑71 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCa < 1:1909951/71‑1 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:3073154/1‑70 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:1986380/1‑69 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4328911/1‑69 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAt > 1:5109870/1‑69 (MQ=255) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc > 1:5371481/1‑71 (MQ=255) tGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCa < 1:4136890/71‑1 (MQ=255) tGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCa < 1:794398/71‑1 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGc < 1:2387368/69‑1 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt > 1:3594575/1‑70 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:280697/1‑71 (MQ=255) ccACGGCGATCAGCAAGTCGCACTACTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATg > 1:1298036/1‑71 (MQ=255) cACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGc < 1:2796935/71‑1 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:2704696/1‑71 (MQ=255) aCGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:1215403/1‑71 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:1739471/71‑1 (MQ=38) gctaTCAGCAAGTCGCAGTCCTGCACCGCGAAGGTCGCCGCTTTTGTGCCATGCATTCCCAGCATGCCCa < 1:1022459/67‑1 (MQ=255) gATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:1537095/1‑70 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:1448791/1‑69 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:5334860/1‑69 (MQ=37) aTCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGAt < 1:5113485/70‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:3310630/55‑1 (MQ=255) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt > 1:4297852/1‑60 (MQ=255) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:1330882/1‑70 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGccc < 1:4413410/63‑1 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:5324460/71‑1 (MQ=37) gcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:1565425/70‑1 (MQ=37) aagTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa > 1:508367/3‑68 (MQ=37) agTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa < 1:3894773/70‑1 (MQ=38) agTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa > 1:5364624/2‑71 (MQ=38) gTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGcc > 1:4296312/1‑57 (MQ=37) gTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGAt < 1:1773325/71‑1 (MQ=38) gcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:1587563/2‑71 (MQ=38) gcacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCAAGAt > 1:4028918/2‑59 (MQ=37) cacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:961088/1‑47 (MQ=37) cacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:799809/1‑47 (MQ=37) cTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCt > 1:2786409/1‑70 (MQ=38) cTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTg > 1:3093832/1‑71 (MQ=39) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:2727545/1‑44 (MQ=37) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAg > 1:4379649/1‑44 (MQ=37) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa < 1:4207456/63‑1 (MQ=38) tGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:3574761/49‑1 (MQ=25) tGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc > 1:2777432/1‑71 (MQ=39) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc > 1:1976025/1‑70 (MQ=38) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc > 1:1691370/1‑70 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGAt > 1:4181068/1‑50 (MQ=25) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:861793/54‑1 (MQ=37) aCCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGc < 1:2158521/39‑1 (MQ=25) aCCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTAc > 1:2925724/1‑71 (MQ=39) ccGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACt > 1:3214357/1‑71 (MQ=39) gcgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgc < 1:48490/71‑1 (MQ=39) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgcg < 1:996074/71‑1 (MQ=39) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgcg > 1:394833/1‑71 (MQ=39) cgAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgcg < 1:2228201/71‑1 (MQ=39) aGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:704986/61‑1 (MQ=38) aGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGccc < 1:3055391/71‑1 (MQ=39) gTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGt > 1:4953853/1‑44 (MQ=21) gTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGcc > 1:3824670/1‑69 (MQ=38) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:3038214/2‑71 (MQ=255) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:4340496/2‑71 (MQ=255) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:1583245/2‑71 (MQ=255) gCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa < 1:2389742/45‑1 (MQ=25) gCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc < 1:293426/70‑1 (MQ=255) ccGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt < 1:329275/48‑1 (MQ=37) cGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCtt < 1:4785917/71‑1 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgcgc > 1:625655/1‑61 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt < 1:1185511/71‑1 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt > 1:159133/1‑71 (MQ=255) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt > 1:2588186/1‑70 (MQ=255) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCt < 1:2649767/68‑1 (MQ=255) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTc < 1:852173/71‑1 (MQ=255) ttGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTgc > 1:2833542/1‑56 (MQ=38) ttGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa > 1:3031134/1‑70 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg > 1:880911/1‑70 (MQ=255) tGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTa < 1:1781747/71‑1 (MQ=255) tGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTa < 1:1413649/71‑1 (MQ=255) | TCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTA > AP009048/3685301‑3685435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |