| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,781 | T→C | 100% | D45D (GAT→GAC) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,781 | 0 | T | C | 100.0% | 177.7 / NA | 50 | D45D (GAT→GAC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/22); total (28/22) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTG > AP009048/3824718‑3824847 | gCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCAc > 1:2853046/1‑71 (MQ=255) cTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACt < 1:3532907/71‑1 (MQ=255) aaaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTg < 1:2766199/69‑1 (MQ=255) aaaTCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGt < 1:248542/70‑1 (MQ=255) cgcAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGttt < 1:1626794/71‑1 (MQ=255) aaaaGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGc > 1:2508111/1‑53 (MQ=255) aaaGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGtt < 1:273040/58‑1 (MQ=255) aaGCCCCTATTTCTTCAACGCAGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACg > 1:44868/1‑71 (MQ=255) cccTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTa < 1:3501968/54‑1 (MQ=255) cTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAg > 1:2978393/1‑71 (MQ=255) tATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAg > 1:193811/1‑70 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCa > 1:3778467/1‑44 (MQ=255) aTTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGca > 1:229556/1‑70 (MQ=255) tttCTTCAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGcat > 1:1927876/1‑69 (MQ=255) ttcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTa < 1:393783/59‑1 (MQ=255) ttcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCt > 1:3157166/1‑63 (MQ=255) tcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTa > 1:2377689/1‑47 (MQ=255) tcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg > 1:140208/1‑71 (MQ=255) cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtgg > 1:17139/1‑71 (MQ=255) cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg < 1:325807/70‑1 (MQ=255) cAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAgg > 1:4415041/1‑45 (MQ=255) aaCGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTa > 1:4857274/1‑42 (MQ=255) cGCCGGGCTGTTTAATACCGGGCGAGACCTGGCACTGTTAGGCCGTTTTTa > 1:129727/1‑51 (MQ=255) cGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg < 1:4334646/71‑3 (MQ=255) cGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg > 1:3756746/1‑69 (MQ=255) gggCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGGGGATTctg < 1:4182921/70‑3 (MQ=255) gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:4169760/71‑1 (MQ=255) gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:2590442/71‑1 (MQ=255) tGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:1805446/1‑71 (MQ=255) gTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:2233035/1‑70 (MQ=255) tttAATACCGGGCGCGACCTTGCACTGTTAGGCCGttttt < 1:3520864/40‑1 (MQ=37) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGa < 1:2857493/59‑1 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:192887/1‑69 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg < 1:1903015/53‑1 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg > 1:2538604/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg < 1:4300840/70‑1 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:712476/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4848177/1‑71 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:4675492/1‑71 (MQ=255) aTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCAtt < 1:2773460/49‑1 (MQ=39) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:2431110/64‑1 (MQ=255) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:2098439/70‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:4772664/1‑62 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAg < 1:2334007/41‑1 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTct < 1:1926450/55‑2 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:2985307/1‑65 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:2087091/1‑71 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:3848493/1‑70 (MQ=255) gcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg > 1:4080204/1‑71 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:3678462/55‑1 (MQ=255) | GCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTG > AP009048/3824718‑3824847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |