| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,814 | G→A | 100% | A56A (GCG→GCA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,814 | 0 | G | A | 100.0% | 264.0 / NA | 88 | A56A (GCG→GCA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (50/38); total (50/38) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCC > AP009048/3824747‑3824880 | tcttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg > 1:140208/1‑71 (MQ=255) cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtgg > 1:17139/1‑71 (MQ=255) cttcAACGCCGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg < 1:325807/70‑1 (MQ=255) cGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg < 1:4334646/71‑3 (MQ=255) cGGGCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg > 1:3756746/1‑69 (MQ=255) gggCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGGGGATTctg < 1:4182921/70‑3 (MQ=255) gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:4169760/71‑1 (MQ=255) gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:2590442/71‑1 (MQ=255) tGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:1805446/1‑71 (MQ=255) gTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:2233035/1‑70 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGa < 1:2857493/59‑1 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:192887/1‑69 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg < 1:1903015/53‑1 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg > 1:2538604/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg < 1:4300840/70‑1 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4848177/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:712476/1‑71 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:4675492/1‑71 (MQ=255) aTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCAtt < 1:2773460/49‑1 (MQ=39) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:2431110/64‑1 (MQ=255) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:2098439/70‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:4772664/1‑62 (MQ=255) cGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTct < 1:1926450/55‑2 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:2985307/1‑65 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:2087091/1‑71 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:3848493/1‑70 (MQ=255) gcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg > 1:4080204/1‑71 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:3678462/55‑1 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATtg < 1:4455023/36‑1 (MQ=38) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTGTGGCATTGAGTTCGATCTGCTGTTTg > 1:351603/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:4226955/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:1284816/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:1067182/2‑71 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTctg < 1:1253243/46‑3 (MQ=39) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:845327/1‑56 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:635506/1‑71 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg < 1:3567822/71‑1 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:2749895/1‑59 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg < 1:171197/65‑1 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc > 1:1702453/1‑71 (MQ=255) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGccc < 1:118273/71‑1 (MQ=255) cACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTct > 1:3320097/1‑40 (MQ=39) cACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCt > 1:2664992/1‑71 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:4553334/51‑1 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:666598/1‑64 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:596226/1‑71 (MQ=255) tGTTAGGACGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:1631080/1‑64 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:3415950/50‑1 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:2413794/1‑52 (MQ=255) ttAGTCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:4945080/1‑53 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:3163664/48‑1 (MQ=39) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:2156782/48‑1 (MQ=39) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:4434284/1‑52 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc < 1:4799212/52‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:3428613/69‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:2211285/1‑71 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt > 1:3016830/1‑58 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:1697141/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:2325296/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:1814887/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCACTGCTTa > 1:3513635/1‑70 (MQ=255) aGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTAc > 1:2222762/1‑70 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:479608/43‑1 (MQ=38) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:1300112/43‑1 (MQ=38) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:4838580/71‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:1732812/71‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:2714805/70‑1 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTAc > 1:3405860/1‑65 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:2514256/1‑70 (MQ=255) tttttACGCTGAAGCATTTGTGGATTCTGGCATTGAGTTCg < 1:4506122/41‑1 (MQ=37) tttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAggg > 1:423753/1‑70 (MQ=255) tttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGa > 1:2415724/1‑71 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:2201471/1‑71 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:4325717/1‑71 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:3931462/1‑71 (MQ=255) tttAAGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATc > 1:2582598/1‑71 (MQ=255) ttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc > 1:1184453/1‑71 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:4962649/70‑1 (MQ=255) aCGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATccc > 1:2850694/1‑70 (MQ=255) cGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCg < 1:4716397/70‑1 (MQ=255) cTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAg < 1:2628073/60‑1 (MQ=255) tGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:4896897/57‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt > 1:365332/1‑51 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:4507356/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:4157267/70‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:3869956/70‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc > 1:3767761/1‑70 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:3663310/70‑1 (MQ=255) | TCTTCAACGCCGGGCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCC > AP009048/3824747‑3824880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |