| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,826 | C→T | 100% | S60S (TCC→TCT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,826 | 0 | C | T | 100.0% | 288.1 / NA | 94 | S60S (TCC→TCT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (56/38); total (56/38) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTG > AP009048/3824760‑3824893 | gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:2590442/71‑1 (MQ=255)gCTGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCa < 1:4169760/71‑1 (MQ=255) tGTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:1805446/1‑71 (MQ=255) gTTTAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:2233035/1‑70 (MQ=255) tttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:192887/1‑69 (MQ=255) ttAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg > 1:2538604/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAg < 1:4300840/70‑1 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:712476/1‑71 (MQ=255) tAATACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt > 1:4848177/1‑71 (MQ=255) aaTACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:4675492/1‑71 (MQ=255) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:2431110/64‑1 (MQ=255) tACCGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:2098439/70‑1 (MQ=255) ccGGGCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt > 1:4772664/1‑62 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:2985307/1‑65 (MQ=255) gggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:2087091/1‑71 (MQ=255) ggCGCGACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:3848493/1‑70 (MQ=255) gcgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg > 1:4080204/1‑71 (MQ=255) cgACCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCAtt < 1:3678462/55‑1 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTGTGGCATTGAGTTCGATCTGCTGTTTg > 1:351603/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:1067182/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:1284816/2‑71 (MQ=255) ccTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTg > 1:4226955/2‑71 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGtt > 1:845327/1‑56 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg < 1:3567822/71‑1 (MQ=255) cTGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:635506/1‑71 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:2749895/1‑59 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATctgctg < 1:171197/65‑1 (MQ=255) tGGCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc > 1:1702453/1‑71 (MQ=255) gCACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGccc < 1:118273/71‑1 (MQ=255) cACTGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCt > 1:2664992/1‑71 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:4553334/51‑1 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:666598/1‑64 (MQ=255) tGTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt > 1:596226/1‑71 (MQ=255) tGTTAGGACGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTgg > 1:1631080/1‑64 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCg < 1:3415950/50‑1 (MQ=255) gTTAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGAt > 1:2413794/1‑52 (MQ=255) ttAGTCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATct > 1:4945080/1‑53 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:2156782/48‑1 (MQ=39) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTc < 1:3163664/48‑1 (MQ=39) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc > 1:4434284/1‑52 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATc < 1:4799212/52‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCt < 1:3428613/69‑1 (MQ=255) ttAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:2211285/1‑71 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGtt > 1:3016830/1‑58 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:1697141/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:1814887/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTa > 1:2325296/1‑70 (MQ=255) tAGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCACTGCTTa > 1:3513635/1‑70 (MQ=255) aGGCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTAc > 1:2222762/1‑70 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:1300112/43‑1 (MQ=38) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGt < 1:479608/43‑1 (MQ=38) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:4838580/71‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:1732812/71‑1 (MQ=255) ggCCGTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa < 1:2714805/70‑1 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTAc > 1:3405860/1‑65 (MQ=255) gTTTTTACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAgg > 1:2514256/1‑70 (MQ=255) tttttACGCTGAAGCATTTGTGGATTCTGGCATTGAGTTCg < 1:4506122/41‑1 (MQ=37) tttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAggg > 1:423753/1‑70 (MQ=255) tttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGa > 1:2415724/1‑71 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:4325717/1‑71 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:3931462/1‑71 (MQ=255) ttttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGAt > 1:2201471/1‑71 (MQ=255) tttAAGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATc > 1:2582598/1‑71 (MQ=255) ttACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc > 1:1184453/1‑71 (MQ=255) tACGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATcc < 1:4962649/70‑1 (MQ=255) aCGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATccc > 1:2850694/1‑70 (MQ=255) cGCTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCg < 1:4716397/70‑1 (MQ=255) cTGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAg < 1:2628073/60‑1 (MQ=255) tGAAGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACaa < 1:4896897/57‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCtt > 1:365332/1‑51 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:4507356/71‑2 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:3663310/70‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc > 1:3767761/1‑70 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:4157267/70‑1 (MQ=255) aaGCATTGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:3869956/70‑1 (MQ=255) gcattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac > 1:339485/4‑71 (MQ=255) gcattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac < 1:2391007/68‑1 (MQ=255) gcattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac < 1:2062068/68‑1 (MQ=255) cattGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcgac > 1:185832/3‑70 (MQ=255) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGc > 1:3048774/2‑40 (MQ=39) attGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGc < 1:2525571/65‑1 (MQ=255) ttGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCg < 1:3804584/60‑1 (MQ=255) ttGGTGGATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcg < 1:2047536/66‑2 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACa > 1:3821343/1‑47 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGa > 1:2227784/1‑59 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcga > 1:4829325/1‑63 (MQ=255) ggtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcga > 1:5002387/1‑63 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGa > 1:1617491/1‑58 (MQ=255) gtggATTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGcga < 1:4380239/64‑3 (MQ=255) tggATTCTGGCATTGAGTTCGATCTGCTGTTTGGc < 1:5315949/35‑1 (MQ=255) ggATTCTGGCATTGAGTTCGATCTGCTGTTTGGccc > 1:1490341/1‑36 (MQ=255) aTTCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGcc > 1:4754386/1‑70 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:3268849/71‑3 (MQ=255) ttCTGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCGACCACTACCGccg < 1:807606/71‑3 (MQ=255) | GCTGTTTAATACCGGGCGCGATCTGGCACTGTTAGGCCGTTTTTACGCTGAAGCGTTGGTGGATTCCGGCATTGAGTTCGATCTGCTGTTTGGCCCTGCTTACAAAGGGATCCCGATTGCCACCACAACCGCTG > AP009048/3824760‑3824893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |