| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,949 | A→G | 100% | E101E (GAA→GAG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,949 | 0 | A | G | 100.0% | 309.8 / NA | 88 | E101E (GAA→GAG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (43/45); total (43/45) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTA > AP009048/3824882‑3825015 | ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:400672/1‑70 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:3644079/71‑1 (MQ=255) cactACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:1886298/71‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:3964653/69‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa < 1:2837012/67‑1 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGa > 1:4187187/1‑70 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:1156487/1‑71 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:2466350/1‑71 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCCTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2610851/67‑1 (MQ=255) gccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCa < 1:4970944/68‑1 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg > 1:1088947/4‑70 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:3004996/67‑1 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGa < 1:299146/68‑1 (MQ=255) cgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:580835/67‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg < 1:1131829/71‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg < 1:4706368/71‑1 (MQ=255) tGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCATAGAGGCAAAAGACCACGGTGAAggc > 1:1031417/1‑71 (MQ=255) tGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc > 1:5191957/1‑71 (MQ=255) tGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc > 1:2513449/1‑71 (MQ=255) cGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc < 1:2471079/71‑1 (MQ=255) ggAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggc < 1:3801430/67‑1 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:2243833/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:4499200/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:2471032/1‑71 (MQ=255) gCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc < 1:818987/71‑1 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCt < 1:3798469/71‑1 (MQ=255) aTCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGAGGCAATCtg < 1:3096047/71‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACgg < 1:568617/54‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg < 1:526134/71‑1 (MQ=255) tCATGAACTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAcc > 1:691059/1‑50 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:2946090/3‑44 (MQ=38) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa > 1:39058/3‑56 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt < 1:3949909/69‑1 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:3490259/3‑71 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt < 1:4933271/69‑1 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt < 1:2792591/68‑1 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttg > 1:5217725/2‑71 (MQ=255) tgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttg < 1:4696915/70‑1 (MQ=255) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACAACGGTGAAGGCGGCAATCtggtt > 1:840854/1‑69 (MQ=255) ttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGCGAAGGCGGCAATCTGGTTGGTAgc > 1:3318635/3‑71 (MQ=255) tgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAgc > 1:4078009/2‑70 (MQ=255) gacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:993983/36‑1 (MQ=255) acctgCCGTACTGCTTTAAACGCAAAGAGGCAAAAGACCACGGTGAAg > 1:909407/1‑48 (MQ=255) ctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg < 1:1173518/58‑1 (MQ=255) ctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGt > 1:3093615/1‑70 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc > 1:1074572/1‑53 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttggt < 1:3775161/61‑1 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCt > 1:4367222/1‑54 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa > 1:3916629/1‑70 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa > 1:2345311/1‑70 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa > 1:1744461/1‑70 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa < 1:4782710/69‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa < 1:4337938/69‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc < 1:1215163/70‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc < 1:5336699/70‑1 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc > 1:2884499/1‑69 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc > 1:545617/1‑69 (MQ=255) gTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg > 1:202600/1‑71 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa < 1:2534755/47‑1 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCa < 1:2563454/46‑1 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAg < 1:2301821/59‑1 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAgg < 1:4950575/71‑1 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg > 1:2480782/1‑70 (MQ=255) tACTGCTTTAACCGCAAAGAGGAAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAgg < 1:1966740/71‑1 (MQ=255) aCTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:4646202/1‑53 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa < 1:259199/45‑1 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGa > 1:3809352/1‑70 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGG‑GGCAATCTGGTTGGTAGCGCGTTACAAGGAc > 1:4536760/1‑70 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgcg > 1:309913/1‑71 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgcg > 1:4169461/1‑71 (MQ=255) cTTTAAACGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:3484779/1‑43 (MQ=37) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGTCAATCt < 1:3820776/42‑1 (MQ=38) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt > 1:1508980/1‑40 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:4877236/69‑1 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTa < 1:3912700/70‑1 (MQ=255) aaCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAg > 1:4219349/1‑50 (MQ=255) aaCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAg > 1:5251904/1‑50 (MQ=255) aaCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa > 1:1973178/1‑70 (MQ=255) aaCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa < 1:537773/70‑1 (MQ=255) aaCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAAt > 1:1632761/1‑71 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGc < 1:2397708/71‑1 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGc < 1:3908496/71‑1 (MQ=255) cGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:1587919/58‑1 (MQ=255) gCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACaa > 1:3421110/1‑56 (MQ=255) gCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg < 1:442783/71‑1 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttg < 1:1561265/41‑1 (MQ=255) aaaGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg > 1:1690109/1‑69 (MQ=255) aaaGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGt < 1:2459163/71‑1 (MQ=255) aagaggCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTa < 1:129244/71‑1 (MQ=255) agaggCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:4693010/1‑68 (MQ=255) | CCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTA > AP009048/3824882‑3825015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |