| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,072 | C→T | 100% | A142A (GCC→GCT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,072 | 0 | C | T | 100.0% | 301.3 / NA | 100 | A142A (GCC→GCT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (36/64); total (36/64) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTT > AP009048/3825004‑3825139 | gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:2175215/1‑71 (MQ=255)gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:2379423/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:944384/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt > 1:4338353/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:442658/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:1754895/71‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:1144436/69‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:209060/69‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:2415777/71‑1 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg < 1:452084/66‑1 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:386110/68‑1 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCt > 1:3187031/1‑71 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:3146230/64‑1 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCtt > 1:4978972/1‑71 (MQ=255) atgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:5218356/61‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:5202710/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:1082817/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:31232/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:4679733/71‑1 (MQ=255) gtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTgg > 1:4114370/1‑71 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:4567979/1‑60 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:4652005/1‑71 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:5218470/1‑71 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:1875520/1‑70 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:2105918/70‑1 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:1230363/71‑2 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta > 1:4874633/1‑70 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:2538699/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:1948340/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:2783251/1‑69 (MQ=255) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:742818/1‑69 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat < 1:396331/70‑3 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:2177988/71‑1 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:2142433/71‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTg > 1:4917043/1‑70 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:2043124/1‑71 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa < 1:729446/71‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCt < 1:2789603/60‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:1516390/71‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:1471559/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc > 1:958458/1‑54 (MQ=39) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:2972446/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt > 1:5151739/1‑71 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:1994214/71‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt < 1:217157/71‑1 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:2567044/45‑1 (MQ=38) ggAACGGCGATTCGCGAATCGATCGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc < 1:3502057/71‑1 (MQ=255) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc < 1:1643196/70‑1 (MQ=255) aaCGGCGATTCGCGAATCGATGGATATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:2550445/71‑1 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:1896724/1‑37 (MQ=37) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:5296478/43‑1 (MQ=38) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGc < 1:3931762/56‑1 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:1073394/71‑3 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa < 1:617120/71‑1 (MQ=255) aTTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:4437258/46‑1 (MQ=38) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2572157/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2181241/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:4122728/1‑71 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAtt > 1:263286/1‑58 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:2429780/1‑70 (MQ=255) cgcgATTCGATGGAGATTATTCAGGCTTATGGCGCGTCGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:5207062/70‑1 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:1929920/1‑71 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:5131789/71‑1 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:1031153/71‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:5022526/68‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:4606113/68‑1 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:2496723/70‑1 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:281851/70‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:606590/43‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:1363748/43‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg > 1:3432087/1‑71 (MQ=255) cGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg > 1:654599/1‑53 (MQ=39) gATGGAGATTATTCAGGCTAATGGCGCGACTCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:39516/1‑63 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg < 1:2709218/71‑3 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:1681475/1‑69 (MQ=255) aTGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:2143935/47‑1 (MQ=38) tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:1448637/1‑62 (MQ=255) tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:664616/71‑1 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg < 1:3523770/71‑1 (MQ=255) agaTTATTCAGGCTAATGGCGCTACGCTTGCTGGCgt < 1:590468/37‑1 (MQ=38) gaTTATTCAGGCTAATGGCGCGACGCTTGCTTGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2299867/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:2463265/50‑3 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGaa < 1:2286496/61‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:875163/1‑63 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:818771/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:2872675/71‑1 (MQ=255) gaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGcgcg < 1:1437815/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1477384/1‑71 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc < 1:3381109/71‑1 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcgg < 1:3169786/70‑1 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1422559/1‑70 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:3994035/1‑70 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:3829640/1‑40 (MQ=38) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:1515632/1‑68 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCgag < 1:45608/71‑1 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:325361/49‑1 (MQ=38) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:1360805/48‑1 (MQ=38) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg > 1:1185276/1‑61 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:515600/71‑1 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2790021/71‑1 (MQ=255) | GTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTT > AP009048/3825004‑3825139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |