Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_pgi1,708,9010AC24.5% 121.9 / 14.8 114R661R (CGA→CGC) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (60/20);  new base C (25/1);  total (88/26)
Fisher's exact test for biased strand distribution p-value = 2.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

CAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACA  >  NC_000913_3_hsa_pgi/1708753‑1709050
                                                                                                                                                    |                                                                                                                                                     
cAGAACACCAGGCTGATCCTCGGAAACCGGCCGTGGAAGCCGCTATTGCCCGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAg                                                                                                                                                      <  1:1849612/150‑1 (MQ=11)
  gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGAAGCAGCTATTGCCAGAGcc                                                                                                                                                    >  1:1367838/1‑150 (MQ=14)
        cAGGTCGATCCTCGCAAATCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAgcg                                                                                                                                              <  1:3131589/150‑1 (MQ=11)
         aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACTCAAGCGGGAACAGCAACAGGATAATGCCGAGCCAGAACAGCAGGTCGATCCGCGCAACGCCGCCCTCGACGCCGCGATTGCACGCGCAAAAgcgc                                                                                                                                             >  1:1939692/1‑150 (MQ=14)
              gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGCCGCGGCTATTGCCCGCGCACAAgccataaaa                                                                                                                                        >  1:1508093/1‑143 (MQ=11)
                      cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCGCAAGCCCTAAAACGCGACCa                                                                                                                                >  2:2994316/1‑150 (MQ=14)
                          gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGACGCCGCTATTGCCCGCGCCCAAGCGCGCACGCGGGagcagccc                                                                                                                            >  1:1887955/1‑148 (MQ=14)
                                gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAGGCCGCGCCAGAGCAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGAGCCAAAGCGCGCGAGCGGGTAGCGCAACAgggt                                                                                                                      >  1:1751433/1‑148 (MQ=14)
                                  cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGACGCCGCTATTGCCCGCGCCAAAGCCCTCAAACCGGCACAGCAGCCGGCTaa                                                                                                                    >  2:3368114/1‑150 (MQ=14)
                                        cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa                                                                                                              <  1:1421453/150‑1 (MQ=255)
                                        cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTTCCCGAGCCAAAGCCAGAAAACCCGAACAGCAGACGGCGAAAGGGGa                                                                                                              >  2:3035814/1‑150 (MQ=14)
                                              tGCCCGTGCCCCAGAACGCACGCCGGAACAGCAACAGGCTAATCCCGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga                                                                                                        <  1:3662282/150‑1 (MQ=17)
                                              ggCCCGTGCCAACGCACGCAAGCTGGACCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCaga                                                                                                        <  1:3415772/149‑1 (MQ=255)
                                               gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGTCGCCGCGGCTATTGCCCGCGCGCACGCCAGCAAAGGGGCACAGCAGACGGCTGATGCCGAGccctaa                                                                                                       >  2:771388/1‑146 (MQ=2)
                                               gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTGGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGCGCCAAGGCGCGCAAAGGGGCCCAGCACCCGGCTATTGCGGAGCCagaa                                                                                                       >  1:2440391/1‑150 (MQ=14)
                                                      ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCCGTAGACCCGGCTATTGCCCGCGCGCAAGCCAGCAAACGGGCGCAGCAAACGGGTTATGAGGAGCCAGAATAAGAgg                                                                                                >  2:4042375/1‑150 (MQ=14)
                                                        aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCAAAAGCGCACAAACGGGAACAGCAACAGGCTTATGCGGAGCCAGAAGAACAGGtt                                                                                              >  2:3794834/1‑150 (MQ=14)
                                                            cACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGAAAACGGGACCAGCAACCGGATAATGCGGAGCCAGAAGAACAGGTTGATc                                                                                          >  1:4099408/1‑150 (MQ=255)
                                                              cGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCACACGCCCGCAAACGCGACCAGCACCCCGCAGAAGCGGAGCCAGAACAGCAGGtgagaccg                                                                                        >  1:583941/1‑143 (MQ=14)
                                                               gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACTGGAACATCAAACGAGAAATGCTCAGCCAGACAAACAGGTTGATCcgc                                                                                       >  1:2604283/1‑150 (MQ=14)
                                                                cAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAAAGCGCGCAACCGCGAACAGCAACCGGCTAATGCGGAGCCCGAAGACCAGGTTGAACcgcc                                                                                      >  1:1233969/1‑149 (MQ=14)
                                                                 aaGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCCCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCAAGAAGAACAGGTAGATCCgcgc                                                                                     >  2:3133015/1‑150 (MQ=255)
                                                                   gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGAAAACGGAAACAGCAACAGGCTAATGAGGAGCAAGAAGAAAAGGTAGATCCGAGCaa                                                                                   >  2:3988437/1‑150 (MQ=14)
                                                                   gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAACGCGCGCAAACCGGAACAGCAACAGGCTCATGCCGAGCCAGAAGAACAGGTAGAACCGCGCaa                                                                                   >  1:1124873/1‑150 (MQ=14)
                                                                        aaCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCCCGCAAACGGGATCAGCAACCTGCTAATGCGGAGCCAGAAGAACAGGTTGATCAGCGCAAAGCTg                                                                              >  2:3021721/1‑150 (MQ=17)
                                                                         aCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAGCCGGCTAATGCGGAGCCAGCAGAACAGGTACCTCCGCGCTCAGCTGc                                                                             >  1:4132988/1‑150 (MQ=17)
                                                                          cAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCAAACGCCCGCAAACGGGAACAGCAAAAGGATAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCGGca                                                                            >  2:1780513/1‑149 (MQ=14)
                                                                           aGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg                                                                           <  1:3165532/150‑1 (MQ=255)
                                                                            gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAATGCGCGCAAACAGGAACAGCAACCGGCTAAATCGGAGACAGAAGACCATGTTGATACCCGCAAagcagcata                                                                          >  1:2022033/1‑147 (MQ=14)
                                                                             cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGACGCGGCTATTGCCCGCGCAAAAGCGAGCAAACGGGAACAGCAAACGGCTAATGCGGAACAAGAGAAACAGGTAGATCCGCGCAAAGCTGCAGTc                                                                         >  2:2288697/1‑150 (MQ=14)
                                                                              atcAGGATAATTCCGACGCAGAACACCTGGTCGATCCTCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAATCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCg                                                                        <  2:496741/148‑1 (MQ=11)
                                                                              aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTAGAAGCGGCTATTGCCCGAGAAAACGCGCGCACACGTGACAAGCAACCTCCAGAGGAGGAGCCAGATGCACACGTAGAGCAGAGAAAAACTGCCGTCg                                                                        >  1:2727396/1‑150 (MQ=9)
                                                                                  ggCTAATGCCGAGCCAGAAACACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGACCCGCAACCGGCTAAAGCGGAGCCAGAAGAACAGTTTGATACGCGGAAAGCTGCAGTACAAGc                                                                    >  1:3812758/1‑150 (MQ=17)
                                                                                    cTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACAGCCTAATGCGGAGCCAGCAGAACAGGTTGATCCGCGCAAAGCTGCGGTCGAAGCgg                                                                  >  2:1075551/1‑150 (MQ=14)
                                                                                     tAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACCGGACCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGCAGCGGc                                                                 >  2:3551913/1‑150 (MQ=17)
                                                                                        tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAt                                                              >  1:3421608/1‑150 (MQ=255)
                                                                                          ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCACAAGCGCGAAAACGGGAACAGCAACAGGATAATGCGGAGCCAGAAGAACAGATTGATCCCCGCAAAGCTGCCGTCTCCGCGGCTATTg                                                            >  1:702783/1‑150 (MQ=14)
                                                                                          ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg                                                            >  2:1968791/1‑150 (MQ=255)
                                                                                           cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGACCAGCACCCGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGAAACGCTGCCGTAGACGCGGCTATTGc                                                           >  1:1809123/1‑150 (MQ=17)
                                                                                           cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc                                                           >  2:1398133/1‑150 (MQ=255)
                                                                                           cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTAGAAGCGGCTATTGc                                                           >  2:3120840/1‑150 (MQ=255)
                                                                                            gAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGcc                                                          >  1:4059570/1‑149 (MQ=255)
                                                                                              gCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCCGCGGCTATTGCCCGCGCGAACGCGCGCACACGGGACCCGCAACCGGCTACTGCGGAGACAGATGAGCAGGTTGATCCGCGCAAAGCTGCCGGCGAGGCGGCTATGGCCcg                                                        >  1:711290/1‑150 (MQ=14)
                                                                                              gCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcg                                                        >  2:3135198/1‑150 (MQ=255)
                                                                                               ccAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGTGCCAGAAGAACAGGTTGATCCGAGCAGCGAAGAGGTCGAAAAGGCTATTGCAcac                                                       >  2:2069388/1‑148 (MQ=14)
                                                                                                cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAGCCGGCTAATGCGGAGCCCGAAGAACAGGTAGATCCGCGCAGAGCTGCCGTCGAAGCGGCTATTGCCcgcg                                                      >  1:3741932/1‑150 (MQ=14)
                                                                                                cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGACCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTCGCAGCGGCTATTGCCcgcg                                                      >  1:151093/1‑150 (MQ=14)
                                                                                                cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCCcgcg                                                      >  2:3053616/1‑150 (MQ=255)
                                                                                                cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgcg                                                      >  2:1328682/1‑150 (MQ=255)
                                                                                                cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgcg                                                      <  1:1968791/150‑1 (MQ=255)
                                                                                                cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCAcgcg                                                      >  2:4097041/1‑150 (MQ=255)
                                                                                                 aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCATCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCAGCAGTAGCAGCGGCTATTGCCCgagc                                                     >  1:525861/1‑150 (MQ=14)
                                                                                                 aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCCAAAGCCAGCAAACGGGAACAGCAACAGGCTAAAACGGAGCCAGAATAACATGTTGATCCGAGCAAAGCGGCAGTAGAAGCGTCTATTGCACgcgc                                                     >  2:544256/1‑150 (MQ=14)
                                                                                                 aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGCACAGCAACCGGCTAATGCGGAGCCAGAAGAACACGTTGATCCGCGCAAATCTGCAGTAGAAGCGGCTATTGCACgcgc                                                     >  1:2779495/1‑150 (MQ=17)
                                                                                                  gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGcc                                                                                                                                                    <  1:3051454/54‑1 (MQ=35)
                                                                                                    acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc                                                                                         >  1:1005662/1‑111 (MQ=255)
                                                                                                    acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc                                                                                         <  2:1005662/111‑1 (MQ=255)
                                                                                                    acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGACAGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCGGTCGGAGGGGCTATGGCCCgcgcgag                                                  >  1:483368/1‑147 (MQ=17)
                                                                                                      aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg                                                                                                 <  1:2732549/101‑1 (MQ=255)
                                                                                                      aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCAGCGGCTATTGCCCGCGCGAAAg                                                >  2:2130285/1‑150 (MQ=17)
                                                                                                        caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACAGCAACAGGATAAAGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCCGCGGCTATTGCACGCGCAAAAGcc                                              >  2:125798/1‑149 (MQ=14)
                                                                                                        caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc                                                                                                                       >  1:2295806/1‑77 (MQ=255)
                                                                                                        caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc                                                                                                                       <  2:2295806/77‑1 (MQ=255)
                                                                                                         aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCACAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAc                                             >  1:2408007/1‑150 (MQ=255)
                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCa                                                                                                          <  2:1489632/88‑1 (MQ=255)
                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCa                                                                                                          >  1:1489632/1‑88 (MQ=255)
                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCAGCAAACGGGACCAGCAACCGGATAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTAGCCGCGGCTATTGCACGCGCACAGGCACg                                            >  2:4204621/1‑150 (MQ=14)
                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCCGAGGCTATTGCACGCGACAACGCCCg                                            >  1:808523/1‑150 (MQ=17)
                                                                                                            tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCa                                          >  2:37613/1‑150 (MQ=255)
                                                                                                              gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAAACGGGAACAGCTACCGGCTAATGCGGAGCCAGAAGAACAGTTTGATCCTCGAAAAGCTGCAGTAGACGCGGCTAATGACCGAGCCAAGGCACTCaat                                        >  2:4078276/1‑149 (MQ=14)
                                                                                                                tCCTCGCAAAGCCTCCTTCGAAGCCGCTATTTCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt                                      <  1:3121472/150‑1 (MQ=14)
                                                                                                                tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAACGCGGAGCCAGAAGAACAGGTTGATCAGCGCTAAGCTGCCGTCGAAGCGGCTAAAGCCCGCGCCAAAGAACGCAAGCt                                      >  1:1562506/1‑150 (MQ=17)
                                                                                                                  cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAACGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg                                    >  1:2440428/1‑150 (MQ=255)
                                                                                                                   gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGa                                   <  1:1399795/150‑1 (MQ=255)
                                                                                                                   gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCACGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGa                                   >  1:3551646/1‑150 (MQ=255)
                                                                                                                     gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAaagcaagcaagcacgacc                                 >  1:1482805/1‑144 (MQ=255)
                                                                                                                     gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGCCCGCTCCAAAGCAAGCAAGAAGGAAc                                 >  2:3046686/1‑150 (MQ=17)
                                                                                                                        aaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGCAGCGGCTATTGCACGCGCCAAAGCCCCCAAGCTGGCACAGc                              >  2:2866053/1‑150 (MQ=17)
                                                                                                                         cccctccgTCGAAGCCGCTATTGCCCGTGACTAAGATCGCAACCGGGACCAGCAACATGCTAATTCGGATACAGTATAACAGTACTATCCGCGCAAAGCAGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCa                             <  2:1751433/145‑1 (MQ=2)
                                                                                                                           ccgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGc                                                                                                                 >  2:2615418/1‑64 (MQ=255)
                                                                                                                           ccgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGc                                                                                                                 <  1:2615418/64‑1 (MQ=255)
                                                                                                                           ccgccgTCGAAGCCGCTATTGCCCGACCCAAAGCGCGCAACCGGGAACAGCAACCCGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAc                           <  1:1075551/150‑1 (MQ=255)
                                                                                                                            cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAGGCACGCAAGCGGGAACAGCAACa                          >  1:2112911/1‑150 (MQ=255)
                                                                                                                            cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCACCa                          >  1:1073194/1‑150 (MQ=255)
                                                                                                                             gccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCAGGAACAGCACCAg                         >  2:1655937/1‑150 (MQ=255)
                                                                                                                                  cGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCCCGCGCCCAAGCACACACGCACGACCAGCAGCAGGCtct                    >  2:294875/1‑148 (MQ=18)
                                                                                                                                  cGAAGCCGCAAGACCCCGAGCCAAACCGCGCAATCGCCAACACCTACCGTCATATGCGGCCCCAGCAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTaa                    <  1:2457789/150‑1 (MQ=14)
                                                                                                                                         ccTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAACCGGCTATTGCACGAGCAAACGCAAGAAAGCTCGCACAGCAAAAGGCAAATGCGGTa             >  2:1303790/2‑150 (MQ=21)
                                                                                                                                          cTATTGCCCGAGCCAAAGCTCGCAACCTGGACCAGCAACCGGCTAATGCCTAGCCAGAAGATCAGGTAGATGCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTAc            <  1:3938787/150‑1 (MQ=17)
                                                                                                                                            aTTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCACGCTGGACCAGCAACAGGCTAATGCGGTACCa          >  1:1663799/1‑150 (MQ=255)
                                                                                                                                             ttGCCCGAGCCAAAGCGATCAATCGGGAACAGCAACTGGCTAATGCTGAGCCATAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCag         <  2:2332715/150‑1 (MQ=17)
                                                                                                                                               gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaa       <  1:1328682/150‑1 (MQ=255)
                                                                                                                                                cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCACCAGGCTAATGCGGTCCCagaag      >  1:1708792/1‑150 (MQ=255)
                                                                                                                                                cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaag      <  1:3135198/150‑1 (MQ=255)
                                                                                                                                                cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGCACGCGCCCAAGCCCGCAAGCAGGACCAGCAACAGGCTGATGAGGTACAagaat      >  2:485269/1‑149 (MQ=25)
                                                                                                                                                cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGAAGCGGCTATTGCCCGCGCCAAAGCCAACAAACACGACCAGCAACAGGTTGATGAGGAAcaataat      >  2:840701/1‑144 (MQ=17)
                                                                                                                                                 ccGTGCCAAACCGCGCAATCGGGAACAGAAACCTGCTAATCCTGACCAAGATCATCAGTTATATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga     <  2:3814806/150‑1 (MQ=14)
                                                                                                                                                 ccGAGCCAAAGCGCGCAAACGGGATCAGCAACCGGCTAATGCGGAGCCAGAAGAACACGATGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATAGCCCGCGCAAAAGCACCAAACCACGAACATCAGCAGGCAAAGGCGGAACCagaaga     >  1:2140025/1‑150 (MQ=255)
                                                                                                                                                  cGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAgaagaa    >  2:3151629/1‑150 (MQ=255)
                                                                                                                                                    aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTCGAACAGCAACAGTCAAATGCGGTACCAGAAGAACa  >  1:53980/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACA  >  NC_000913_3_hsa_pgi/1708753‑1709050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑