| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_pgi | 1,708,655 | 0 | A | G | 20.3% | 95.8 / 19.0 | 66 | E579E (GAA→GAG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base A (14/33); new base G (7/5); total (25/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCC > NC_000913_3_hsa_pgi/1708509‑1708781 | cGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGATcc > 1:1461822/1‑149 (MQ=255)cGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAcc < 1:1476599/149‑1 (MQ=255) gTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCa < 2:1614038/149‑1 (MQ=255) atcgcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaac < 2:48833/147‑1 (MQ=255) aGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaac < 1:58375/149‑1 (MQ=255) gcgcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCGAGCGGCTTATGCGGGACGAGacca > 2:825438/1‑149 (MQ=38) gcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaacaac < 1:1209018/149‑1 (MQ=255) gcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaacaac < 1:747045/149‑1 (MQ=255) gcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGaacaac > 1:978899/1‑149 (MQ=255) cAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTCGAACAGCAACAGCCTAATGCGGAACCAGAacaaca > 2:1322430/1‑149 (MQ=255) aGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAgg < 1:343754/149‑1 (MQ=255) gCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGt < 2:56667/149‑1 (MQ=255) gCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGt < 2:257012/149‑1 (MQ=255) aactgagCGAACCAGGAACAACTTCAGGAAACTCACGACGCAGCACAACATGCTGATCCTCGTAAATCTGCCGTAGAAGCAGCTATAGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGt < 1:190866/145‑1 (MQ=14) gCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATcc < 2:580251/149‑1 (MQ=255) aaCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGTCTAATGCGGAACCAGAACAACAGGTCGATCCgcgc < 1:1201896/149‑1 (MQ=255) cAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCaa < 1:603413/149‑1 (MQ=255) gCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGATCTTCAGGTCGATCGgcgctatgc > 2:888167/1‑144 (MQ=25) gCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAgc > 1:875581/1‑149 (MQ=255) gCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAgc > 2:142489/1‑149 (MQ=255) gCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAgc < 1:142489/149‑1 (MQ=255) gAACTGATGCAAACTAACGAAGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAgccgc < 2:1531082/149‑1 (MQ=255) caAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGc < 2:638304/149‑1 (MQ=255) tAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGTCGCCGCTa > 1:1426319/1‑149 (MQ=255) cgacgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCAAAACGCGAAAACCCTCCGAAGAAGCCCCTAtag > 1:1555808/1‑147 (MQ=21) gccgCAGCAACCGTTGCAGATCCACCTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc < 2:916067/147‑1 (MQ=255) gacgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATAGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACAAGAACAACAGGTCGATCCGCGCAAAGCCGCCATCGGAACCGCTaaggg > 2:1563529/1‑145 (MQ=18) acgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTGATGCGGAGCCCGCACGTCAGGTCGCACCGCGCGAGGCGGCCGGCGAAGCCGCTTTTGcc > 1:559788/1‑149 (MQ=18) ccGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCACCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa < 1:485579/149‑1 (MQ=255) tGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgc < 1:1537797/149‑1 (MQ=255) gtGATCCACGTAAAACTGCCGTTTAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcgc < 1:1386451/148‑1 (MQ=255) tCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGc < 2:735102/149‑1 (MQ=255) ctcgaaaaaCTGCCGTAGAAGCAGCTATTGCCCGGGCCTAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg < 1:543627/144‑1 (MQ=11) aCGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACAGGTCGATACCCGCACACACCCCGTCGAACCCGCTATGGCCCGGGCCAACGCGCGCAAGCTgg > 1:1182494/1‑149 (MQ=17) gTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGaa < 1:964748/149‑1 (MQ=255) tAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGAAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACAGGTCGAACCGCGCAAAGCCGCCGGCGAAGCCGCTAGTGCGCGGGCGAACGCGAGCGAGCTGGagc > 1:1004567/1‑147 (MQ=17) aaCTGCCGTTGAAGCAGCTAGCGCCCGCGCCACCGCGCGCAAGCTGGAACAGCAACAGCCTAATGCGGAACCAGAACACCAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGc < 1:888167/149‑1 (MQ=14) tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg > 2:985235/1‑80 (MQ=255) tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg < 1:985235/80‑1 (MQ=255) gCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACa < 1:1627195/149‑1 (MQ=255) gCCGTTGAAGCAGCTATCGACCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACa < 2:20276/149‑1 (MQ=255) gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt < 2:1332571/74‑1 (MQ=255) gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt > 1:1332571/1‑74 (MQ=255) gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt > 1:298278/1‑74 (MQ=255) gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt > 1:304054/1‑74 (MQ=255) gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt < 2:298278/74‑1 (MQ=255) gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt < 2:304054/74‑1 (MQ=255) cTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg < 2:201708/84‑1 (MQ=18) cTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAgccg > 1:201708/1‑84 (MQ=255) aaTCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc < 2:20688/148‑1 (MQ=255) ctcacgcgACAAAGCGCGCAATCTGGAACATCAACAGGCTAATTCGGAACCAGAACAACATGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCag < 1:706804/145‑1 (MQ=14) cccccCGCCCACGCGCGCAAGCAGGAACAGCATCAGCCTACTCCCGACCCAGCACCCCAGGCCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATAGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCaga < 2:1421703/148‑1 (MQ=14) cacccggcCAAAGCGCGCAATCTGGCACAGCAACAGGCAACTGAGGAACCAGAACAACATGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCaga < 2:573603/143‑1 (MQ=14) cccGCGCCATATCTAGCAATCTGGAACAGCAACAGGATAATTCGGAACCATAAAAACATGGCGATCCTCGTAAAGCTGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaa < 2:875581/149‑1 (MQ=14) cccGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGaacaac > 2:1260761/1‑57 (MQ=255) cacgctcgcAATCAGGAACATCATCAGGATAATGAGGAACCAGAACAACTTGTCGATCCGCGAAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGt < 1:1322430/143‑1 (MQ=14) aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc < 1:858048/89‑1 (MQ=18) aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc > 2:858048/1‑89 (MQ=33) gcgcgcAAGCTGGAACAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg < 2:1652503/79‑1 (MQ=21) gcgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg > 1:1652503/1‑79 (MQ=34) gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGaa > 1:1017347/1‑109 (MQ=16) aaGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGc < 2:935282/71‑1 (MQ=34) aaGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGACGAAGc > 1:935282/1‑71 (MQ=21) tGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCAGTCGAGGCCGCTATTGAGCGTGCGAAAGCGCGGAAGCTGGAGCAGGAAGCGGCTACTGCGGAGCCAGAAGAGCAGGTCGATACGCGaaaa > 2:596230/1‑149 (MQ=11) aactgtaaCATGCTTATGCGGAAGCAGAACAAGTGGTTGATCCTCGAAAATCTGCCGTAGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgcc < 1:1563529/143‑1 (MQ=14) cAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAgc > 2:1472191/1‑83 (MQ=18) | CGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCC > NC_000913_3_hsa_pgi/1708509‑1708781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |