Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,546,3910CT71.4% 121.0 / 61.5 84intergenic (+192/‑168)nfuA/gntTFe/S biogenesis protein, putative scaffold/chaperone protein/gluconate transporter, high‑affinity GNT I system
Reads supporting (aligned to +/- strand):  ref base C (9/15);  new base T (22/38);  total (31/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGAC  >  NC_000913_3_pae_pgi/3546243‑3546525
                                                                                                                                                    |                                                                                                                                      
tCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCg                                                                                                                                       <  2:538162/150‑1 (MQ=255)
  aTTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTa                                                                                                                                     >  1:551932/1‑150 (MQ=255)
  aTTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTa                                                                                                                                     <  2:454804/150‑1 (MQ=255)
   tttCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTAt                                                                                                                                    <  4:207036/150‑1 (MQ=255)
    ttCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATc                                                                                                                                   <  4:705912/150‑1 (MQ=255)
          ccTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTcac                                                                                                                             <  3:675719/150‑1 (MQ=255)
           cTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTcaca                                                                                                                            <  1:770629/150‑1 (MQ=255)
             aCGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATg                                                                                                                          <  3:586879/150‑1 (MQ=255)
              cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATgg                                                                                                                         >  2:869448/1‑150 (MQ=255)
              cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATgg                                                                                                                         <  1:869448/150‑1 (MQ=255)
              cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATgg                                                                                                                         <  4:538710/150‑1 (MQ=255)
               gTTGGACATCGGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAAGCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATggg                                                                                                                        <  3:597145/150‑1 (MQ=255)
               gTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATggg                                                                                                                        <  1:145337/150‑1 (MQ=255)
               gTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATggg                                                                                                                        <  3:597146/150‑1 (MQ=255)
               gTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTATCCCCGCGATAATATGACCAAGCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATggg                                                                                                                        >  1:49666/1‑150 (MQ=255)
                  gAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTAc                                                                                                                     >  2:1066608/1‑150 (MQ=255)
                     cATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCaa                                                                                                                  >  4:386951/1‑150 (MQ=255)
                          gCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATa                                                                                                             <  3:526018/150‑1 (MQ=255)
                           ccAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATAc                                                                                                            >  3:532621/1‑150 (MQ=255)
                             aGACGTTGGCGCCGCATTGCTTTGTCAACTCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAACCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTc                                                                                                          <  2:49666/150‑1 (MQ=255)
                              gACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTcc                                                                                                         <  4:440057/150‑1 (MQ=255)
                                 gTTGGCGCAGCATTGCTTTGTCAAATCCTGGGTTCGTTTCTTCGCGATAATATGACCAGCCTCGCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCGTATGTTACCTGTATCTTTCACATGGGTACCAAACAAACTCCTGa                                                                                                      <  2:506094/150‑1 (MQ=255)
                                 gTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGa                                                                                                      <  3:342457/150‑1 (MQ=255)
                                     gcgcAGCATTGCTTCGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATc                                                                                                  <  4:690576/150‑1 (MQ=255)
                                       gcagcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTg                                                                                                >  4:564248/1‑150 (MQ=255)
                                        cagcaTTGCTTTGTCAAATCCTGGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATCGGTACCAAACATACTCCTGACATCTgt                                                                                               <  2:749438/150‑2 (MQ=255)
                                         agcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGAc                                                                                              <  2:135071/150‑1 (MQ=255)
                                               gCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaat                                                                                        <  1:217570/150‑1 (MQ=255)
                                                cTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaata                                                                                       >  1:125006/1‑150 (MQ=255)
                                                cTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACACGGGTCGGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaata                                                                                       >  1:320720/1‑150 (MQ=255)
                                                 tttGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaataa                                                                                      <  2:679050/150‑1 (MQ=255)
                                                  ttGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaataat                                                                                     <  1:93096/150‑1 (MQ=255)
                                                   tGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAAtt                                                                                    <  1:282136/150‑1 (MQ=255)
                                                    gTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTa                                                                                   <  3:277099/150‑1 (MQ=255)
                                                     tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAg                                                                                  <  3:283758/150‑1 (MQ=255)
                                                     tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAg                                                                                  <  1:182789/150‑1 (MQ=255)
                                                       aaaTCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGtt                                                                                >  4:474956/1‑150 (MQ=255)
                                                          tCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGACTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTa                                                                             >  3:726221/1‑150 (MQ=255)
                                                            cTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGt                                                                           <  2:532066/150‑1 (MQ=255)
                                                              cGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTgg                                                                         >  2:680498/1‑150 (MQ=255)
                                                              cGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTgg                                                                         >  3:364265/1‑150 (MQ=255)
                                                               ggTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGCGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTggg                                                                        <  3:466676/150‑1 (MQ=255)
                                                                  tCGTTTGCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTAt                                                                     <  1:628513/150‑1 (MQ=255)
                                                                  tCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGGAt                                                                     >  4:645331/1‑150 (MQ=255)
                                                                    gTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCa                                                                   >  1:103919/1‑150 (MQ=255)
                                                                         cccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgt                                                              >  2:507548/1‑150 (MQ=255)
                                                                         cccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgt                                                              >  4:51718/1‑150 (MQ=255)
                                                                         cccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgt                                                              >  2:357217/1‑150 (MQ=255)
                                                                         cccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgt                                                              >  4:12151/1‑150 (MQ=255)
                                                                          ccGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtg                                                             <  2:919372/150‑1 (MQ=255)
                                                                           cgcgATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtgg                                                            >  2:1101367/1‑150 (MQ=255)
                                                                           cgcgATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCgtgg                                                            <  4:364265/150‑1 (MQ=255)
                                                                              gATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATa                                                                                                             <  2:993709/98‑1 (MQ=255)
                                                                              gATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATa                                                                                                             >  1:993709/1‑98 (MQ=255)
                                                                                 aataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGc                                                      <  1:296183/150‑1 (MQ=255)
                                                                                 aataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGc                                                      <  3:432327/150‑1 (MQ=255)
                                                                                      gACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGa                                                                                               <  1:136202/104‑1 (MQ=255)
                                                                                      gACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGa                                                                                               >  2:136202/1‑104 (MQ=255)
                                                                                        ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATAtct                                               >  4:154944/1‑150 (MQ=255)
                                                                                         cAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATAtctc                                              >  2:207938/1‑150 (MQ=255)
                                                                                          atccTCTCATAATTTAAATTGACCGCGCTCGGGTGATTCTCAAACGCCAGATGTGACCTGTATCATTCACATGGGTACCAAACATACTCCTGACTTCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATctct                                             <  2:2967/148‑1 (MQ=255)
                                                                                          aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATctct                                             <  1:1142268/150‑1 (MQ=255)
                                                                                               ctcATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTcc                                        <  3:609732/150‑1 (MQ=255)
                                                                                                tcATAATTTAAATTTACCCCTCTCTGCTTATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACGTCTGACTACAATAATTAGTTTGAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTccc                                       <  2:862387/150‑1 (MQ=255)
                                                                                                    aatttaaatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGAtt                                   >  2:509121/1‑150 (MQ=255)
                                                                                                      tttaaatttaCCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg                                 <  2:125006/150‑1 (MQ=255)
                                                                                                              taCCCCGCTCTGGTGACTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTa                         <  4:726221/150‑1 (MQ=255)
                                                                                                                ccccGCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTaga                       <  3:269915/150‑1 (MQ=255)
                                                                                                                 cccGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTACTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTagag                      >  4:735181/1‑150 (MQ=255)
                                                                                                                   cGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGtt                    <  3:12151/150‑1 (MQ=255)
                                                                                                                    gCTCTGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGttt                   <  1:929675/150‑1 (MQ=255)
                                                                                                                     ctctGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTg                  <  3:495152/150‑1 (MQ=255)
                                                                                                                      tctGGTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgt                 <  2:522711/150‑1 (MQ=255)
                                                                                                                       ctGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtc                <  1:1162712/150‑1 (MQ=255)
                                                                                                                          gTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcgtc             <  3:588489/150‑1 (MQ=255)
                                                                                                                          gTGATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTgtcgtc             >  2:358074/1‑150 (MQ=255)
                                                                                                                            gATTCTCAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGGTTGTCGTCAg           >  1:357632/1‑150 (MQ=255)
                                                                                                                               tctcAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGaaa        >  2:576255/1‑150 (MQ=255)
                                                                                                                               tctcAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGaaa        >  3:539651/1‑150 (MQ=255)
                                                                                                                                 tcAAACGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAAt      <  1:235135/150‑1 (MQ=255)
                                                                                                                                   aaaCGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGCCTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTg    >  1:681015/1‑150 (MQ=255)
                                                                                                                                    aaCGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGa   <  1:357217/150‑1 (MQ=255)
                                                                                                                                    aaCGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGa   <  1:1066608/150‑1 (MQ=255)
                                                                                                                                     aCGCCAGATGTTACCTGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTGCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGAc  <  2:547583/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
TCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGAC  >  NC_000913_3_pae_pgi/3546243‑3546525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: