Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	4,159,085	G→T	C199F (TGT→TTT)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	4,159,085	0	G	T	96.9%	342.5 / 0.0	97	C199F (TGT→TTT)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (50/44);  total (51/46)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCG  >  NC_000913_3_pae_pgi/4158939‑4159230                                                                                                                                                   |                                                                                                                                                  agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTtttt                                                                                                                                                <  1:1169327/150‑1 (MQ=255) agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTtttt                                                                                                                                                <  3:348221/150‑1 (MQ=255)             aagcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGAAAAAAAAAAAAAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGc                                                                                                                                    <  4:176525/147‑1 (MQ=255)              ttcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCa                                                                                                                                   >  1:382555/1‑150 (MQ=255)                 attGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATg                                                                                                                                >  3:508371/1‑150 (MQ=255)                 attGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATg                                                                                                                                >  4:508759/1‑150 (MQ=255)                  ttGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGCTCAGGCAATgg                                                                                                                               >  1:369102/1‑150 (MQ=255)                  ttGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAAACGCGACTGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATgg                                                                                                                               <  3:417722/150‑1 (MQ=255)                         gCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTg                                                                                                                        <  4:48956/150‑1 (MQ=255)                          ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGt                                                                                                                       <  2:229382/150‑1 (MQ=255)                            gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGCTGGTCACTTTTTTCGCGATCAGGCAATGGGTTTCTGttt                                                                                                                     >  2:1181871/1‑150 (MQ=255)                            gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                                                                     >  4:512671/1‑150 (MQ=255)                             ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                                                    <  4:427/150‑1 (MQ=255)                                 ttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAg                                                                                                                >  2:512105/1‑150 (MQ=255)                                  tgatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGc                                                                                                               >  2:931816/1‑150 (MQ=255)                                   gatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGcc                                                                                                              >  3:300276/1‑150 (MQ=255)                                     tgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgg                                                                                                            <  2:1157694/150‑1 (MQ=255)                                       aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                                                          <  3:512671/150‑1 (MQ=255)                                       aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                                                          >  1:989105/1‑150 (MQ=255)                                        gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                                                         >  1:338423/1‑150 (MQ=255)                                        gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                                                         <  1:1118581/150‑1 (MQ=255)                                        gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                                                         <  4:746826/150‑1 (MQ=255)                                         ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCg                                                                                                        >  3:477132/1‑150 (MQ=255)                                           aaTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                                                                      <  2:982799/150‑1 (MQ=255)                                              gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGa                                                                                                   >  2:1012656/1‑150 (MQ=255)                                                tGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAg                                                                                                 <  4:638185/150‑1 (MQ=255)                                                   tGGCTATCTATGAAGATCACCCGTGGGCGAACCTCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                                              <  2:227913/150‑1 (MQ=255)                                                       tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca                                                                                          <  2:474332/150‑1 (MQ=255)                                                       tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                                              >  1:108070/1‑146 (MQ=255)                                                       tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                                              <  2:108070/146‑1 (MQ=255)                                                        atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                                         >  4:571133/1‑150 (MQ=255)                                                            atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                                                                     >  3:555501/1‑150 (MQ=255)                                                             tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                                                    >  2:563811/1‑150 (MQ=255)                                                               aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcg                                                                                  <  1:1012656/150‑1 (MQ=255)                                                                aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                                                                     <  3:84627/114‑1 (MQ=255)                                                                aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                                                                     >  4:84627/1‑114 (MQ=255)                                                                 gATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgcg                                                                                >  2:285164/1‑150 (MQ=255)                                                                  aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa                                                                               >  1:990272/1‑150 (MQ=255)                                                                     aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCa                                                                            <  4:41627/150‑1 (MQ=255)                                                                       ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                                                                          >  3:213017/1‑150 (MQ=255)                                                                         gTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCt                                                                        >  1:847017/1‑150 (MQ=255)                                                                            ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                                                     >  4:509074/1‑150 (MQ=255)                                                                            ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                                                     <  3:129845/150‑1 (MQ=255)                                                                            ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                                                     >  2:804116/1‑150 (MQ=255)                                                                              ggAACCGCGAATGCGTACCGATGGCCGACCTGTCAGGGGAAAAACTGCTGAGGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa                                                                   <  1:657713/149‑1 (MQ=255)                                                                                   cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTgc                                                                                                                                              >  2:1213445/1‑70 (MQ=255)                                                                                   cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTgc                                                                                                                                              <  1:1213445/70‑1 (MQ=255)                                                                                   cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                                                              >  1:990086/1‑150 (MQ=255)                                                                                    gcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                             >  4:398553/1‑150 (MQ=255)                                                                                     cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATGCACACTTCCGCGCGACCAGCCTGGACACTCTgcg                                                            >  2:170805/1‑150 (MQ=255)                                                                                      gAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcgc                                                           >  3:353084/1‑150 (MQ=255)                                                                                             tACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTTTTTTTTTTTGCAATGGGTTTCTGTTTTGAAGCCGGGTCGGATGAAGATACACACCTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                    >  4:501368/1‑150 (MQ=255)                                                                                             tACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                    <  2:665834/150‑1 (MQ=255)                                                                                              aCCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggt                                                   >  1:921519/1‑150 (MQ=255)                                                                                              aCCGATGGCCGATCCGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggt                                                   <  2:961107/150‑1 (MQ=255)                                                                                              aCCGATGGCCGATCCGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggt                                                   <  2:463275/150‑1 (MQ=255)                                                                                                cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                                 >  1:994989/1‑150 (MQ=255)                                                                                                  aTGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg                                               >  1:692807/1‑150 (MQ=255)                                                                                                    ggCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcggc                                             <  4:508371/150‑1 (MQ=255)                                                                                                      ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTTTGTGTTTTTGCAATGGATTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCGGCGCAACAGGGTGGCGGCAg                                           >  4:517530/1‑150 (MQ=255)                                                                                                      ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                           <  2:739389/150‑1 (MQ=255)                                                                                                       cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAgg                                          >  1:588070/1‑150 (MQ=255)                                                                                                        gATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                            >  4:620972/1‑131 (MQ=255)                                                                                                        gATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                            <  3:620972/131‑1 (MQ=255)                                                                                                              gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                   <  1:563811/150‑1 (MQ=255)                                                                                                                aGGGGGAGAGCTGCGGATGCTGGAAGAGGGTCACGGTTGGCGCGATCAGGCAATGGGTATCTGTCTTGAAGGCGGTGCTGATGAAGATACACACGTCCGCGCGATGAGCCTGGAAACTCTGCGCAACAGGGTGGCGGCAGGCAGCGGGAt                                 <  4:730827/150‑1 (MQ=255)                                                                                                                aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAGGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                 >  1:465655/1‑150 (MQ=255)                                                                                                                aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                 <  1:285164/150‑1 (MQ=255)                                                                                                                aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                 <  3:357524/150‑1 (MQ=255)                                                                                                                aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTCCCGCGCGACCGGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                 <  4:260970/150‑1 (MQ=255)                                                                                                                 ggggAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTTTTTTTTTTTTCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                >  4:258451/1‑150 (MQ=255)                                                                                                                 ggggAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                >  4:360537/1‑150 (MQ=255)                                                                                                                  gggAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                    >  4:731400/1‑129 (MQ=255)                                                                                                                  gggAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                    <  3:731400/129‑1 (MQ=255)                                                                                                                   ggAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                              <  2:1229970/150‑1 (MQ=255)                                                                                                                       aaaCTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa                          <  3:508759/150‑1 (MQ=255)                                                                                                                       aaaCTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa                          <  1:504781/150‑1 (MQ=255)                                                                                                                        aaCTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTAc                         <  2:994989/150‑1 (MQ=255)                                                                                                                        aaCTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTAc                         >  2:829469/1‑150 (MQ=255)                                                                                                                          ctgctgATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTg                       >  3:297912/1‑150 (MQ=255)                                                                                                                             ctgATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCa                    <  1:931816/150‑1 (MQ=255)                                                                                                                              tgATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAg                   >  2:1200263/1‑150 (MQ=255)                                                                                                                               gATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgc                  >  1:312375/1‑150 (MQ=255)                                                                                                                                    tGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg             <  3:398553/150‑1 (MQ=255)                                                                                                                                    gggAAGATGGTCGCTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCCGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg             <  2:345213/149‑1 (MQ=255)                                                                                                                                    gggAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGCTTCTGTTTTGAAGCCGGCGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCCACATGGTCGCGGCAGGTAGCGGGATCACGTTACTGCCAGCactgg             >  2:943589/2‑150 (MQ=255)                                                                                                                                        aGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt         >  4:12012/1‑150 (MQ=255)                                                                                                                                           tGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgcc      <  2:382555/150‑1 (MQ=255)                                                                                                                                           tGGTCACGTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgcc      <  2:338423/150‑1 (MQ=255)                                                                                                                                            ggTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccg     >  3:95091/1‑150 (MQ=255)                                                                                                                                             gTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc    <  3:571133/150‑1 (MQ=255)                                                                                                                                              tCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTTCTGCCAGCGCTGGCTGTgccgcc   <  2:369102/150‑1 (MQ=255)                                                                                                                                              tCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgcc   >  4:766519/1‑150 (MQ=255)                                                                                                                                              tCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgcc   <  1:1200263/150‑1 (MQ=255)                                                                                                                                              tCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgcc   >  4:592895/1‑150 (MQ=255)                                                                                                                                              tCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgcc   >  1:648937/1‑150 (MQ=255)                                                                                                                                               cACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                         <  1:702469/95‑1 (MQ=255)                                                                                                                                               cACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                         >  2:702469/1‑95 (MQ=255)                                                                                                                                               cACTTTTTGCGCGATCAGGCAATGGGTGTCTGGTTTGAGGCCGGGGCGGGTGAAGATACACGCTTCCGCGCGTCCATGCTGGAAGTTCTGCGCAACATGGTGACGGCAGGTAGGGGGATCACTTTGCTGCCAGCGCTGGCTGTGccgccg  <  4:577093/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  AGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCG  >  NC_000913_3_pae_pgi/4158939‑4159230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑