Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_pgi526,8000AG37.2% 40.4 / 35.6 44E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (6/21);  new base G (4/12);  total (11/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCG  >  NC_000913_3_vch_pgi/526654‑526940
                                                                                                                                                   |                                                                                                                                            
cAGGGCGGGCTGACGGTGGAGCAACTGGCCAGCCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAata                                                                                                                                            <  1:77837/149‑1 (MQ=255)
 aGTGCGGGCTGACGTTGGCTCAACTGGCCAGACTGGTGGAGTCGTAATACACTCCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                           <  1:177261/149‑1 (MQ=17)
   tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGCGCCGGAATACACACCGGCGCGAAAAAC‑TAATATTTATAAATGAGACCACCGGGGACTGACGCTGGCGCTTATCAGCGAAAACGGCAATACGGAGTGGGGCGGGGGGTatga                                                                                                                                         >  1:20153/1‑149 (MQ=11)
   tGCGGGCGGAGGGTGGAGCAGTGGGCCAGACAGGTGGCGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                         <  2:328561/149‑1 (MQ=17)
    gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                        <  2:117256/149‑1 (MQ=255)
    gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                        <  1:268763/149‑1 (MQ=255)
       ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGaa                                                                                                                                     <  2:197317/149‑1 (MQ=255)
        gCTGACGGTGGGGCAACTGGCCAGACAGGTGGAGCAGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                                    <  1:56699/149‑1 (MQ=11)
          tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                  <  1:159478/149‑1 (MQ=255)
          tGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                  <  1:7495/149‑1 (MQ=255)
           gccGGTGGAGTAACGGGCCAGACAGGTGGAGGCGGAATACACACCGGTGCGGAAAGC‑TCATCTTTATCACTGGGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                 <  2:361811/147‑1 (MQ=11)
           gccGGTGGAGCAACTGGCCATACAGTTGGATCCGGAATACCCACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                 <  1:43727/147‑1 (MQ=11)
           gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATGCACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                 <  1:301842/149‑1 (MQ=255)
           gACGGCGGAGCAACTGGCCAGACCGGTGGAGCCGGCATGCAC‑CCCGAGAAACCGCCTTCACCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGGGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                 <  1:108561/149‑1 (MQ=14)
                tGGAGTAACGGGCCATACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGATCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                            <  1:83628/149‑1 (MQ=11)
                           gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                 <  1:236183/149‑1 (MQ=255)
                             cAGACCGGTGGAGCCGGACTACACACCGGCGCGAAAAGC‑TCATCTTTATCGCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAAGACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagt                                                                                                               <  1:203361/149‑2 (MQ=11)
                              agagagGTGGACGCGGAATACCCACAGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                              <  2:210619/149‑1 (MQ=17)
                              aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGTCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                              <  1:151219/149‑1 (MQ=255)
                                aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGgaga                                                                                                            >  1:330040/1‑149 (MQ=255)
                                 cAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGaa                                                                                                           >  1:110628/1‑149 (MQ=255)
                                    gTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatg                                                                                                                                          <  2:168095/115‑1 (MQ=11)
                                    gTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatg                                                                                                                                          >  1:168095/1‑115 (MQ=255)
                                    gTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGAGCGCACCGGGGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                        <  1:303678/149‑1 (MQ=255)
                                     tGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATAGGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCg                                                                                                       >  1:666/1‑149 (MQ=255)
                                       gAGCGGGACTACACACCGGCGCGAAAAGC‑TCCTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCCTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGAAGTATTATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                     <  2:162855/149‑1 (MQ=11)
                                         gCCGGAATACAGACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                   >  1:237225/1‑149 (MQ=255)
                                          cctgAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                                  <  1:273951/146‑1 (MQ=255)
                                            ggAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtg                                                                                                <  2:242039/149‑1 (MQ=255)
                                                 agaacccGGCGCGAAAAGG‑TCATTTTTATTACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                           <  2:378743/144‑1 (MQ=255)
                                                  cacacCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                          <  1:85781/149‑1 (MQ=255)
                                                                       aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAATATGATGAATGGGGCGACCAGCTTAATGAGGAGAACCCGCATCATGTGTGTCGGGCGTGACCTCTACCAggg                                                                      >  1:35646/1‑149 (MQ=17)
                                                                         cTTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgca                                                                    <  1:162881/149‑1 (MQ=255)
                                                                            tATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                 <  1:23786/149‑1 (MQ=255)
                                                                              tCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCCGAGAAGACGGCAATACAGCGTGGAGCGCGGACTATGATGAATGGGGCAACCGGCTAACAGCGGAGAACCCGCATCATGTGCGTCAGCCGTACCGACTGCCAGGgcagcgcc                                                               >  2:203006/1‑146 (MQ=255)
                                                                                   gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                          <  1:318290/149‑1 (MQ=255)
                                                                                        caccGGGGACTGCCGCTGGCGCTTATAAGCGAAGATAGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                     >  1:384860/1‑149 (MQ=255)
                                                                                                aCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGc                                             <  1:324170/149‑1 (MQ=255)
                                                                                                    ccGCTGGAGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                         <  1:183052/149‑1 (MQ=255)
                                                                                                          gcgcTCATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                   <  1:27948/149‑1 (MQ=255)
                                                                                                          gcgcTAATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGCATATGCTGAATGGGGCAACCAGCATAACGCGCATAACACGCATCATGTGGATCTGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                   <  1:286575/149‑1 (MQ=17)
                                                                                                            gcTTATCCGCGAAGAAGGCAATCCGCCACGAAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCTAGATTAGGAATCAGGGCTGTACTATAAcc                                 >  2:177234/1‑149 (MQ=17)
                                                                                                                         gACGGCAATACGGCGTGGACCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                    <  1:199532/149‑1 (MQ=255)
                                                                                                                            ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                     <  1:212391/49‑1 (MQ=255)
                                                                                                                            ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                     >  2:212391/1‑49 (MQ=255)
                                                                                                                                           tgcgcTGAATATCATGAATGGTGGGACCTGTGTATGGACTAGAACCCGCATCATGTTTATTGGCCGTACCGTCTGCGATGGCAGGCGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCg  <  1:177234/148‑1 (MQ=12)
                                                                                                                                                   |                                                                                                                                            
CAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCG  >  NC_000913_3_vch_pgi/526654‑526940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑