Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_tpiA2,214,1520AT29.2% 80.5 / 27.3 48Y165N (TAT→AAT) yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base A (13/21);  new base T (8/6);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAA  >  NC_000913_3_vch_tpiA/2214012‑2214298
                                                                                                                                            |                                                                                                                                                  
aGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCggg                                                                                                                                            <  2:600840/149‑1 (MQ=255)
aGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCggg                                                                                                                                            <  1:371194/149‑1 (MQ=255)
  tGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGAGCGGCGCCGACGGTATCGGTGCCTGACCGTAGCGGAACTATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGAGCg                                                                                                                                          >  1:148621/1‑149 (MQ=255)
    gCAGAAAAAGCCGAAGTGTACTTTTGAAGCAGCGCGCGGTTATCGTGCAATATACAGATAAGCCGCGCCTCGCCGATCTTATGTGTGGCCATCCTTCGTGCAGTAATATTACTCACCAGACGCACCGCATCTTCATTCGGTCGGGCGAt                                                                                                                                        <  2:698872/149‑1 (MQ=255)
     cAGAAAAAGCCGTAGTGTATTTTTCACACAGCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCACTATTCCTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATc                                                                                                                                       <  2:265006/149‑1 (MQ=255)
          aaaGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAg                                                                                                                                  <  2:625043/149‑1 (MQ=255)
               cGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTAACCATCATTGCCGCAGCAAGATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGgatga                                                                                                                             >  2:733446/1‑149 (MQ=255)
                gAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGgatgat                                                                                                                            <  1:672606/149‑1 (MQ=255)
                 aattgtATTTTTCAAACACCGCGCGTTTATCGACCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATc                                                                                                                           <  1:240564/146‑1 (MQ=255)
                     gtATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCa                                                                                                                       <  2:333440/149‑1 (MQ=255)
                        tttttCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGGCGGGCGATCGCAAGGATGATCAGCAttt                                                                                                                    >  1:482346/1‑149 (MQ=255)
                                   tCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCa                                                                                                         <  1:407963/149‑1 (MQ=255)
                                          tttATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGcgac                                                                                                    <  2:552643/147‑1 (MQ=255)
                                          tttATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGcgac                                                                                                    >  1:552643/1‑147 (MQ=255)
                                            tATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAAc                                                                                                >  2:611906/1‑149 (MQ=255)
                                            tATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAAGATTACGCACCAGACCCACCGCAACTTCATAAGGCGGGGCGAACGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAAc                                                                                                >  2:1194/1‑149 (MQ=255)
                                             aTCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACg                                                                                               >  2:190916/1‑149 (MQ=255)
                                                gAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTGCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGCCGCActaga                                                                                            >  1:569778/1‑145 (MQ=255)
                                                  gtaaTATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGAACATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCCTCATAAGGTCGGGCGATCGCAAGGATGAGCAGCATTTGCACCATTTCAGCGACGAACGTGACg                                                                                          <  2:639327/147‑1 (MQ=255)
                                                  gCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGCCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCAGGCGATCACCAGGCAGACCATCCTTTCCCCCATTTCACCCACGAGCgcgccg                                                                                          >  2:41655/1‑144 (MQ=255)
                                                          cGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACcggc                                                                                  <  1:73820/149‑1 (MQ=255)
                                                           gCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGCCGGCACcggcg                                                                                 >  1:335845/1‑149 (MQ=255)
                                                             aTAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGCCGAACGTGACGGCCCCGGCGGt                                                                               >  1:406542/1‑149 (MQ=255)
                                                                aaCAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAAt                                                                            >  2:469286/1‑149 (MQ=255)
                                                                  cAGCGCCGCGCCGGCGGTATTGGGGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATgg                                                                          <  2:541079/149‑1 (MQ=255)
                                                                   aGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTCCTCCCTAGACGTACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATggg                                                                         <  2:394535/149‑1 (MQ=255)
                                                                                 gttagtGGTGAGCATGATTGGCGGAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTAt                                                           <  2:335845/144‑1 (MQ=255)
                                                                                   tATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCa                                                         >  2:348321/1‑149 (MQ=255)
                                                                                           aCCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGccc                                                 >  1:306589/1‑149 (MQ=255)
                                                                                             catcatTGGCGCAGCAATATTACTCACCAGACGGACCACATCTTCATATGGGTGTGCGAGCGCACGGATGATCAGCACTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCcgc                                               <  2:196869/149‑1 (MQ=255)
                                                                                             catcatTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCcgc                                               >  1:577349/1‑149 (MQ=255)
                                                                                               tcatTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCgcgg                                             <  2:304570/149‑1 (MQ=255)
                                                                                                       gcagcaATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGCCGGATCAg                                     >  2:409644/1‑149 (MQ=255)
                                                                                                       gcagcaATATTACTCACAAGACGCACCGCATCTTCATAAGGTCGAGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTACGCCCGCGGCAGAGCAg                                     >  1:115190/1‑149 (MQ=255)
                                                                                                           caATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATg                                 <  2:569235/149‑1 (MQ=255)
                                                                                                              tatTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCtg                              <  1:190916/149‑1 (MQ=255)
                                                                                                                  aCTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTa                          <  1:22460/149‑1 (MQ=255)
                                                                                                                    tCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATTAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGGTGAAGACTTTATCAGTGCGCCCGCGGCGGATCCGGATGCTGTGTAcc                        >  1:221671/1‑149 (MQ=255)
                                                                                                                     cACCAGACGCACCCCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCa                       <  1:272663/149‑1 (MQ=255)
                                                                                                                        cAGACGCACCGCATCTTCATTAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGc                    <  1:348321/149‑1 (MQ=255)
                                                                                                                               aCCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAg             >  1:716652/1‑149 (MQ=255)
                                                                                                                                 cacATCTTCATAAGGTCGGGCGATCGCCCGGACGAGCCGCATTTGCAACATTTCAGCGAGGAACTTGACGCCACCGCCGGTAATGGGCTTAAAAAACTTCTCAGAGCACCCCCTGCGGATCAGGATGCTATATACCAAGCCACCGAGTa           >  2:227635/3‑149 (MQ=255)
                                                                                                                                 cGCATCTTCATTAGGTCGGGCGATTGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTa           <  1:398763/149‑1 (MQ=255)
                                                                                                                                 cGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTa           >  1:669584/1‑149 (MQ=255)
                                                                                                                                       ttCATTAGGTCGGGCGATCGGAAGGCTTATGAGCATTCGAAACAATTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCtt     <  2:519400/149‑1 (MQ=255)
                                                                                                                                       ttCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCtt     <  1:433822/149‑1 (MQ=255)
                                                                                                                                        tCATTAGGTCGCGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTc    <  1:312470/149‑1 (MQ=255)
                                                                                                                                          aTAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCaa  <  1:611906/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
AGTGGCAGAAAAAGCCGAAGTGTATTTTTCAAACATCGCGCGTTTATCGAGCAATATACGCATAAACAGCGCCGCGCCGACGGTATTGGTGACCATCATTGGCGCAGCAATATTACTCACCAGACGCACCGCATCTTCATAAGGTCGGGCGATCGCAAGGATGATCAGCATTTGCACCATTTCAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAA  >  NC_000913_3_vch_tpiA/2214012‑2214298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑