Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_vch_tpiA	1,196,247	A→G	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_vch_tpiA	1,196,247	0	A	G	100.0%	27.0 / NA	13	L375M (TTA→CTG)	icd	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/8);  total (5/8)

ACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGG  >  NC_000913_3_vch_tpiA/1196102‑1196314                                                                                                                                                  |                                                                    aCATCGGTGACGAATGGGCCCTGTTTGAAGGCACGCACGGTACTGCGCCGAAATATGCAGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                  <  2:395268/149‑1 (MQ=38)   aTCAGTAACGAATAACCCCTGTTTGAAGCCACCCACGGTACTGCGCCAAAATAAGCCGCTCAGGACAAAGTAAATCCAGGCTCTATTATTCCCTCCGCTGAGATGATGCTGCGCCATATGGGTTAGACTGAAGCGGCTGACCTGATTGt                                                                <  2:10302/149‑1 (MQ=25)      ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCACCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                                             >  2:34650/1‑149 (MQ=38)       gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                            >  1:374739/1‑149 (MQ=255)       gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                            <  2:217537/149‑1 (MQ=255)                    ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                               >  1:369807/1‑149 (MQ=255)                            aaGCCAACCACGGTAATGCGCCGAAATATGCCGGCCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                       <  2:196012/149‑2 (MQ=25)                              gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATTATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTGAAAGGTATGGAAGGCGCAATCAAtgc                                     <  1:34650/149‑1 (MQ=25)                                 acccacGGTACTGCGCCGAAATATGCCGGTCACGACAAAGTTAATCCTGGCTGTATTATGCGCTCCGTTGAGATGATGGTGCGCCAAATGGTTTGGACTGAAGCGGCTGACCTGATTCTTAAAGGTATGGAAGGCGACATCAATGCCaa                                  <  2:297459/149‑1 (MQ=18)                                                          ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                    >  1:434246/1‑106 (MQ=21)                                                          ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                    <  2:434246/106‑1 (MQ=21)                                                              tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga     <  2:374739/149‑1 (MQ=14)                                                                 ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAGCGTCTgatgg  >  1:117475/1‑149 (MQ=14)                                                                                                                                                  |                                                                    ACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGG  >  NC_000913_3_vch_tpiA/1196102‑1196314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑