| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 4,159,190 | T→A | L224Q (CTG→CAG) | oxyR → | oxidative and nitrosative stress transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 4,159,190 | 0 | T | A | 96.3% | 82.4 / NA | 27 | L224Q (CTG→CAG) | oxyR | oxidative and nitrosative stress transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (18/8); minor base C (1/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTT > NC_000913_3_vch_tpiA/4159057‑4159333 | tGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCCCGCGACCAGCCAGGAAACTCTGCGCaa > 2:33227/1‑149 (MQ=255) gATCTGGCAGGGGAACAACTGCTGCTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGACACTCTTCGCAAACTGGTAGCGGCAgg > 2:237359/1‑149 (MQ=255) gATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAgg > 1:87525/1‑149 (MQ=255) gATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAgg > 1:86683/1‑149 (MQ=255) tCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATAAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCCCAACATGGTGGCGACCGGtc > 2:127996/1‑148 (MQ=255) gCAGGGGAAAAACTGCTGATGCTGGAAGACGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCgg < 1:422535/149‑1 (MQ=255) aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTAc > 1:272002/1‑149 (MQ=255) tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGGGAATGAAGATACAGCCTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACCTGGTGGCCGAACGTAACAGGAACGCtttcccg > 2:231277/1‑145 (MQ=255) ctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGAAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCCGGAAACTCTGCGCAACATGGTGGGGGCAGGTAGGGGGATGCCTTTCCTGcc > 2:191091/1‑149 (MQ=255) gATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAg > 1:138364/1‑149 (MQ=255) tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACGGCCAGCgctg > 2:88398/1‑149 (MQ=255) tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTGTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctg > 2:592821/1‑149 (MQ=255) tGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCCCCggg > 2:504451/1‑148 (MQ=255) tttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc < 1:420527/149‑1 (MQ=255) gTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgtt > 1:485736/1‑149 (MQ=255) gTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgtt < 1:440525/149‑1 (MQ=255) aaGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc > 1:147502/1‑149 (MQ=255) gggtgATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGGGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGGACGCGCGATGGGGTTGTTTATCTGCCGTGCATTAAGc < 2:316983/145‑1 (MQ=255) gCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGcc < 1:417749/149‑1 (MQ=255) cGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCg < 1:388937/149‑1 (MQ=255) aTACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc < 1:413105/99‑1 (MQ=255) aTACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc > 2:413105/1‑99 (MQ=255) acacacTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTATTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAGGCCGGAAGCAcgccg > 2:554689/1‑149 (MQ=255) acacTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCa > 1:614927/1‑149 (MQ=255) acTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGCCGGCAGGTAGCGGGAACACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACTCCATGGGGCAGTTTATCTCCCGTGCATTAAGCCGGCACCCCGCTGCCCt > 2:360449/1‑149 (MQ=255) aCCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGt < 1:592821/149‑1 (MQ=255) cAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGttt > 1:401685/1‑149 (MQ=255) | TGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTT > NC_000913_3_vch_tpiA/4159057‑4159333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |