breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 32 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,815,811 | 0 | C | A | 62.5% | 18.8 / 21.8 | 24 | intergenic (‑43/+52) | pyrE/rph | orotate phosphoribosyltransferase/truncated RNase PH |
* | NC_000913 | 2,177,245 | 0 | G | T | 61.3% | ‑1.7 / 13.1 | 34 | intergenic (‑41/+267) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 3,233,720 | 0 | A | C | 60.0% | ‑0.8 / 11.5 | 15 | intergenic (+37/+8) | fadH/higA | 2,4‑dienoyl‑CoA reductase/antitoxin/DNA‑binding transcriptional repressor HigA |
* | NC_000913 | 2,177,221 | 0 | T | G | 56.4% | 6.4 / 42.6 | 39 | intergenic (‑17/+291) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 3,750,793 | 0 | G | C | 53.3% | 1.2 / 14.0 | 15 | intergenic (+12/+335) | sgbE/yiaT | L‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT |
* | NC_000913 | 2,177,234 | 0 | C | A | 52.0% | 7.4 / 25.3 | 39 | intergenic (‑30/+278) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 4,220,007 | 0 | T | G | 52.0% | ‑6.6 / 18.2 | 25 | V471G (GTG→GGG) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 4,220,016 | 0 | A | T | 50.0% | ‑3.4 / 16.2 | 20 | Y474F (TAT→TTT) | aceK | isocitrate dehydrogenase kinase/phosphatase |
* | NC_000913 | 2,177,255 | 0 | G | T | 48.6% | ‑0.2 / 26.6 | 37 | intergenic (‑51/+257) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 1,879,846 | 0 | T | G | 48.3% | 17.1 / 14.2 | 29 | T35P (ACG→CCG) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 4,392,453 | 0 | C | T | 45.5% | ‑0.6 / 11.1 | 11 | intergenic (+18/‑19) | glyV/glyX | tRNA‑Gly/tRNA‑Gly |
* | NC_000913 | 1,879,848 | 0 | C | G | 44.8% | 23.1 / 12.8 | 29 | G34A (GGA→GCA) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 238,523 | 0 | G | T | 38.0% | 35.7 / 10.4 | 51 | L72I (CTT→ATT) | ykfM | uncharacterized protein YkfM |
* | NC_000913 | 2,177,228 | 0 | C | G | 37.0% | 26.7 / 12.1 | 40 | intergenic (‑24/+284) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 3,004,946 | 0 | G | T | 35.6% | 55.8 / 16.5 | 45 | Q281K (CAA→AAA) | ygeV | putative sigma(54)‑dependent transcriptional regulator YgeV |
* | NC_000913 | 1,757,197 | 0 | C | A | 35.5% | 49.9 / 10.2 | 31 | intergenic (+87/‑224) | pykF/lpp | pyruvate kinase I/murein lipoprotein |
* | NC_000913 | 2,982,476 | 0 | C | A | 35.3% | 74.4 / 16.4 | 51 | intergenic (‑294/+21) | araE/kduD | arabinose:H(+) symporter/putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase |
* | NC_000913 | 3,448,232 | 0 | T | A | 33.3% | 55.8 / 10.1 | 39 | intergenic (‑83/+82) | rplN/rpsQ | 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17 |
* | NC_000913 | 4,236,265 | 0 | A | G | 31.8% | 62.7 / 16.6 | 48 | K2E (AAG→GAG) | yjbF | lipoprotein YjbF |
* | NC_000913 | 931,029 | 0 | G | C | 31.4% | 52.4 / 10.9 | 35 | intergenic (‑67/+56) | cydD/trxB | glutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4498181 = | NA (NA) | 12 (0.250) | 7/256 | 3.3 | NA | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 4499512 | NA (NA) | intergenic (+12/+81) | insD‑6/yjgX | KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment | |||||
* | ? | NC_000913 | 381258 = | NA (NA) | 21 (0.450) | 7/254 | 3.3 | NA | pseudogene (3/417 nt) | yaiX | putative acyltransferase, N‑terminal fragment |
? | NC_000913 | = 382589 | NA (NA) | noncoding (1330/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 742317 = | 41 (0.870) | 3 (0.070) | 3/236 | 4.6 | 8.5% | coding (240/1482 nt) | dtpD | dipeptide:H(+) symporter DtpD |
? | NC_000913 | = 3603736 | 27 (0.620) | coding (508/1494 nt) | ftsY | signal recognition particle receptor | |||||
* | ? | NC_000913 | = 2308296 | 40 (0.850) | 3 (0.070) | 3/240 | 4.7 | 8.8% | coding (320/1644 nt) | yojI | ABC transporter family protein/microcin J25 efflux protein |
? | NC_000913 | = 3955908 | 25 (0.560) | coding (578/1545 nt) | ilvA | threonine deaminase |