breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8110CA62.5% 18.8 / 21.8 24intergenic (‑43/+52)pyrE/rphorotate phosphoribosyltransferase/truncated RNase PH
*NC_0009132,177,2450GT61.3% ‑1.7 / 13.1 34intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,233,7200AC60.0% ‑0.8 / 11.5 15intergenic (+37/+8)fadH/higA2,4‑dienoyl‑CoA reductase/antitoxin/DNA‑binding transcriptional repressor HigA
*NC_0009132,177,2210TG56.4% 6.4 / 42.6 39intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,750,7930GC53.3% 1.2 / 14.0 15intergenic (+12/+335)sgbE/yiaTL‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT
*NC_0009132,177,2340CA52.0% 7.4 / 25.3 39intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009134,220,0070TG52.0% ‑6.6 / 18.2 25V471G (GTG→GGG) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009134,220,0160AT50.0% ‑3.4 / 16.2 20Y474F (TAT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009132,177,2550GT48.6% ‑0.2 / 26.6 37intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009131,879,8460TG48.3% 17.1 / 14.2 29T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
*NC_0009134,392,4530CT45.5% ‑0.6 / 11.1 11intergenic (+18/‑19)glyV/glyXtRNA‑Gly/tRNA‑Gly
*NC_0009131,879,8480CG44.8% 23.1 / 12.8 29G34A (GGA→GCA) yeaRDUF1971 domain‑containing protein YeaR
*NC_000913238,5230GT38.0% 35.7 / 10.4 51L72I (CTT→ATT) ykfMuncharacterized protein YkfM
*NC_0009132,177,2280CG37.0% 26.7 / 12.1 40intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,004,9460GT35.6% 55.8 / 16.5 45Q281K (CAA→AAA) ygeVputative sigma(54)‑dependent transcriptional regulator YgeV
*NC_0009131,757,1970CA35.5% 49.9 / 10.2 31intergenic (+87/‑224)pykF/lpppyruvate kinase I/murein lipoprotein
*NC_0009132,982,4760CA35.3% 74.4 / 16.4 51intergenic (‑294/+21)araE/kduDarabinose:H(+) symporter/putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
*NC_0009133,448,2320TA33.3% 55.8 / 10.1 39intergenic (‑83/+82)rplN/rpsQ50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
*NC_0009134,236,2650AG31.8% 62.7 / 16.6 48K2E (AAG→GAG) yjbFlipoprotein YjbF
*NC_000913931,0290GC31.4% 52.4 / 10.9 35intergenic (‑67/+56)cydD/trxBglutathione/L‑cysteine ABC exporter subunit CydD/thioredoxin reductase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4498181 =NA (NA)12 (0.250) 7/256 3.3 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 381258 =NA (NA)21 (0.450) 7/254 3.3 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 742317 =41 (0.870)3 (0.070) 3/236 4.6 8.5% coding (240/1482 nt) dtpD dipeptide:H(+) symporter DtpD
?NC_000913 = 3603736 27 (0.620)coding (508/1494 nt) ftsY signal recognition particle receptor
* ? NC_000913 = 230829640 (0.850)3 (0.070) 3/240 4.7 8.8% coding (320/1644 nt) yojI ABC transporter family protein/microcin J25 efflux protein
?NC_000913 = 3955908 25 (0.560)coding (578/1545 nt) ilvA threonine deaminase