breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,746 G→T 9.3% D139E (GAC→GAA caiD ← carnitinyl‑CoA dehydratase
RA 41,794 G→T 10.8% Y46* (TAC→TAA caiT ← putative transporter
RA 95,432 G→T 6.3% P181P (CCG→CCT ftsW → lipid II flippase; integral membrane protein involved in stabilizing FstZ ring during cell division
RA 170,006 G→T 12.8% intergenic (+75/+83) fhuB → / ← hemL fused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components/glutamate‑1‑semialdehyde aminotransferase (aminomutase)
RA 170,019 G→A 13.5% intergenic (+88/+70) fhuB → / ← hemL fused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components/glutamate‑1‑semialdehyde aminotransferase (aminomutase)
RA 179,593 C→T 5.7% A215V (GCG→GTG)  cdaR → carbohydrate diacid regulon transcriptional regulator; autoregulator
RA 198,641 G→A 7.7% P18P (CCG→CCA fabZ → (3R)‑hydroxymyristol acyl carrier protein dehydratase
RA 212,605 G→A 5.4% intergenic (+139/+61) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
RA 218,062 G→A 5.5% T16M (ACG→ATG)  metI ← DL‑methionine transporter subunit
RA 221,425 T→C 6.7% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 6.9% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 222,173 T→C 6.3% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA of rrnH operon
RA 250,297 C→A 10.4% pseudogene (109/501 nt) prfH → pseudogene, RF‑1 domain family; probable peptide chain release factor
RA 262,526 C→T 13.0% pseudogene (153/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 262,529 C→T 11.8% pseudogene (150/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 262,559 C→T 12.9% pseudogene (120/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 262,565 A→G 11.6% pseudogene (114/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 263,285 G→A 7.4% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 274,084 G→A 5.2% R144C (CGC→TGC)  afuC ← CP4‑6 prophage; putative ferric transporter subunit
RA 302,342 C→T 5.7% G63S (GGT→AGT)  ecpD ← polymerized tip adhesin of ECP fibers
RA 309,261 G→T 7.2% intergenic (‑308/+166) ykgR ← / ← ykgP uncharacterized protein/pseudogene, oxidoreductase family
RA 345,326 T→C 5.1% intergenic (+43/‑397) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 345,353 A→G 5.9% intergenic (+70/‑370) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 347,206 C→T 8.2% A94V (GCG→GTG)  prpD → 2‑methylcitrate dehydratase
RA 353,317 C→T 7.5% intergenic (+152/+185) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 362,443 G→A 6.2% R71* (CGA→TGA)  mhpR ← mhp operon transcriptional activator
RA 370,428 T→C 7.3% intergenic (+91/‑487) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,430 A→G 5.3% intergenic (+93/‑485) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,442 T→C 9.4% intergenic (+105/‑473) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,576 Δ1 bp 7.5% intergenic (+239/‑339) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,577 T→G 8.7% intergenic (+240/‑338) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,632 A→G 6.4% intergenic (+295/‑283) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 372,807 Δ1 bp 8.2% intergenic (+40/+184) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,808 G→A 8.1% intergenic (+41/+183) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 389,367 C→A 5.7% pseudogene (942/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 410,069 G→A 5.4% H381Y (CAT→TAT)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 445,212 C→T 89.2% G296S (GGT→AGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 463,374 T→C 6.9% R169R (CGT→CGC cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
RA 492,608 C→A 6.9% intergenic (+158/‑23) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
RA 494,564 C→T 7.3% G289D (GGC→GAC)  aes ← acetyl esterase
RA 526,113 C→A 8.7% A190A (GCG→GCT mnmH ← tRNA 2‑selenouridine synthase, selenophosphate‑dependent
RA 539,163 C→T 5.4% G114S (GGC→AGC)  allE ← S‑ureidoglycine aminohydrolase
RA 573,883 G→C 13.0% T107T (ACG→ACC
R34P (CGG→CCG) 
rzpD →
rzoD →
DLP12 prophage; putative murein endopeptidase
DLP12 prophage; putative lipoprotein
RA 583,819 G→T 8.2% G864G (GGC→GGA nfrA ← bacteriophage N4 receptor, outer membrane subunit
RA 605,602 A→G 9.9% intergenic (‑67/+108) entD ← / ← fepA phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter
RA 605,605:1 +A 7.8% intergenic (‑70/+105) entD ← / ← fepA phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter
RA 611,208 G→A 7.1% W532* (TGG→TGA entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 648,609 C→A 6.0% R307S (CGT→AGT)  citA → sensory histidine kinase in two‑component regulatory system with CitB
RA 697,528 C→A 8.6% R227L (CGT→CTT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 726,319 G→C 5.1% A427A (GCG→GCC rhsC → Rhs family putative polymorphic toxin
RA 727,171 T→G 7.2% N711K (AAT→AAG rhsC → Rhs family putative polymorphic toxin
RA 727,174 C→T 7.0% G712G (GGC→GGT rhsC → Rhs family putative polymorphic toxin
RA 727,207 C→T 8.3% R723R (CGC→CGT rhsC → Rhs family putative polymorphic toxin
RA 727,285 C→G 6.4% R749R (CGC→CGG rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 6.4% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 729,930 A→G 7.0% pseudogene (342/1521 nt) rhsO → pseudogene, Rhs family protein
RA 733,986 A→G 5.5% K147E (AAG→GAG)  ybfD → H repeat‑associated putative transposase
RA 733,994 C→T 6.4% S149S (TCC→TCT ybfD → H repeat‑associated putative transposase
RA 734,003 C→T 5.6% I152I (ATC→ATT ybfD → H repeat‑associated putative transposase
RA 787,254 C→A 5.8% V86V (GTG→GTT galE ← UDP‑galactose‑4‑epimerase
RA 811,070 A→G 9.3% intergenic (+67/+125) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,071 T→C 9.3% intergenic (+68/+124) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,072 T→C 9.4% intergenic (+69/+123) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,073 T→A 9.3% intergenic (+70/+122) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 856,008 G→T 11.3% T685T (ACC→ACA ybiW ← putative pyruvate formate lyase
RA 904,679 C→T 7.3% P24P (CCG→CCA ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 913,880 C→A 6.7% A233S (GCC→TCC)  ybjX ← DUF535 family protein
RA 920,211 G→A 6.4% G498R (GGG→AGG)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 931,482 G→T 14.6% G935C (GGT→TGT)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 941,426 G→A 6.9% A34T (GCC→ACC)  ycaD → putative MFS‑type transporter
RA 947,571 C→G 6.5% M480I (ATG→ATC) ‡ pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 947,573 T→G 6.7% M480L (ATG→CTG) ‡ pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 947,576 G→T 7.4% R479S (CGC→AGC)  pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 947,577 C→G 6.5% E478D (GAG→GAC pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 960,205 G→T 5.9% D144Y (GAT→TAT)  ycaI → ComEC family inner membrane protein
RA 1,001,641 G→T 17.4% V78V (GTG→GTT zapC → FtsZ stabilizer
RA 1,026,004 G→A 5.6% P41S (CCG→TCG)  tusE ← mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 1,045,283 T→G 10.0% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,047,768 G→T 5.8% Q351K (CAA→AAA)  yccM ← putative 4Fe‑4S membrane protein
RA 1,053,762 G→T 5.3% A75S (GCT→TCT)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,083,810 G→T 7.0% Q502K (CAA→AAA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
RA 1,105,819 G→T 7.2% W343C (TGG→TGT opgG → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
RA 1,186,761 G→T 5.4% Q228K (CAG→AAG)  purB ← adenylosuccinate lyase
RA 1,197,850 T→C 8.0% E180E (GAA→GAG cohE ← e14 prophage; repressor protein phage e14
RA 1,204,059 C→T 5.2% A173T (GCT→ACT)  tfaE ← e14 prophage; putative tail fiber assembly protein
RA 1,218,978 C→T 6.6% intergenic (+73/‑42) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,254,576 G→T 7.8% intergenic (+51/+4) ychH → / ← dauA DUF2583 family putative inner membrane protein/C4‑dicarboxylic acid transporter
RA 1,255,129 G→A 7.8% S377S (TCC→TCT dauA ← C4‑dicarboxylic acid transporter
RA 1,265,875 T→C 6.2% noncoding (27/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,265,888 T→G 6.3% noncoding (40/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,268,123 C→A 5.5% S54* (TCG→TAG)  chaC → cation transport regulator
RA 1,282,249 A→G 8.8% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 7.1% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,495 T→G 6.5% intergenic (+513/+27) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,497 A→G 8.3% intergenic (+515/+25) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,498 G→A 5.6% intergenic (+516/+24) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,288,276 C→A 91.9% V35F (GTT→TTT)  hns ← global DNA‑binding transcriptional dual regulator H‑NS
RA 1,302,400 G→A 5.4% A168V (GCT→GTT)  clsA ← cardiolipin synthase 1
RA 1,351,604 G→T 7.0% intergenic (‑237/+76) sapA ← / ← ymjA antimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein
RA 1,355,936 C→A 6.8% A187E (GCG→GAG)  puuD → gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
RA 1,403,860 C→A 7.3% A31A (GCC→GCA dbpA → ATP‑dependent RNA helicase, specific for 23S rRNA
RA 1,417,031 T→C 8.3% pseudogene (74/500 nt) ydaW → Rac prophage; pseudogene, DNA‑binding protein family;Phage or Prophage Related
RA 1,417,147 G→T 10.8% pseudogene (190/500 nt) ydaW → Rac prophage; pseudogene, DNA‑binding protein family;Phage or Prophage Related
RA 1,422,060 G→C 8.7% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 6.3% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 6.0% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 5.4% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 6.2% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,798 A→G 5.8% pseudogene (186/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,435,640 G→T 6.0% Q121K (CAG→AAG)  hslJ ← heat‑inducible lipoprotein involved in novobiocin resistance
RA 1,460,819 G→A 8.1% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,477,945 C→A 5.6% L210I (CTC→ATC)  hrpA → putative ATP‑dependent helicase
RA 1,532,695 C→A 7.0% W139L (TGG→TTG)  narY ← nitrate reductase 2 (NRZ), beta subunit
RA 1,546,447 T→C 17.1% intergenic (+199/+208) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,546,448 C→T 16.7% intergenic (+200/+207) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,593,583 C→A 7.1% R295L (CGC→CTC)  lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,639,188 C→T 6.3% intergenic (‑29/+188) rem ← / ← hokD Qin prophage; uncharacterized protein/Qin prophage; small toxic polypeptide
RA 1,663,488 G→T 5.0% P121H (CCT→CAT)  ynfL ← LysR family putative transcriptional regulator
RA 1,666,107 Δ1 bp 5.2% coding (74/84 nt) ydgU → stationary phase‑induced protein
RA 1,672,050 G→A 10.0% S37S (AGC→AGT pntA ← pyridine nucleotide transhydrogenase, alpha subunit
RA 1,681,696 A→G 11.0% G313G (GGT→GGC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,729 G→A 6.4% R302R (CGC→CGT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,732 A→G 6.1% I301I (ATT→ATC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,735 C→A 6.3% V300V (GTG→GTT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,741 G→A 6.3% I298I (ATC→ATT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,696,039 G→T 17.2% A276S (GCC→TCC)  malY → bifunctional beta‑cystathionase, PLP‑dependent/ regulator of maltose regulon
RA 1,702,966 T→C 9.7% R597R (CGT→CGC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,710,193 C→A 9.3% intergenic (‑47/+12) pdxY ← / ← tyrS pyridoxamine kinase/tyrosyl‑tRNA synthetase
RA 1,712,204 C→A 6.3% G21W (GGG→TGG)  pdxH ← pyridoxine 5'‑phosphate oxidase
RA 1,717,339 G→T 7.2% C321F (TGC→TTC)  ydhK → putative efflux protein (PET) component of YdhJK efflux pump
RA 1,720,418 C→A 7.8% H47N (CAC→AAC)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,760,891 G→T 5.6% P684P (CCC→CCA ydiJ ← putative FAD‑linked oxidoreductase
RA 1,785,882 G→T 8.7% S132S (TCC→TCA ydiV ← anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase
RA 1,789,696 C→A 7.5% L38L (CTG→CTT ihfA ← integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,845,784 C→G 6.7% A223G (GCT→GGT)  ansA → cytoplasmic L‑asparaginase 1
RA 1,851,318 C→T 5.8% A237T (GCA→ACA)  ydjI ← putative aldolase
RA 1,868,111 C→T 5.9% A94V (GCC→GTC)  yeaK → aminoacyl‑tRNA editing domain protein
RA 1,875,344 Δ1 bp 7.5% coding (723/924 nt) dmlR ← transcriptional activator of dmlA
RA 1,881,943 G→T 9.8% D102E (GAC→GAA rnd ← ribonuclease D
RA 1,944,104 G→T 6.4% Q226K (CAG→AAG)  aspS ← aspartyl‑tRNA synthetase
RA 1,945,807 G→A 5.2% E52E (GAG→GAA yecE → UPF0759 family protein
RA 1,948,722 A→G 5.6% intergenic (+52/+113) cmoB → / ← torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,948,724 C→T 6.7% intergenic (+54/+111) cmoB → / ← torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,948,730 T→A 9.5% intergenic (+60/+105) cmoB → / ← torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
RA 2,024,400 G→T 7.3% H467N (CAT→AAT)  dcm ← DNA cytosine methyltransferase
RA 2,054,474 C→A 5.1% intergenic (‑79/+23) cbl ← / ← nac ssuEADCB/tauABCD operon transcriptional activator/nitrogen assimilation regulon transcriptional regulator; autorepressor
RA 2,059,656 T→C 10.3% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,122,049 G→T 6.6% C107* (TGC→TGA wcaF ← putative acyl transferase
RA 2,135,624 C→T 5.4% V25M (GTG→ATG)  dcd ← 2'‑deoxycytidine 5'‑triphosphate deaminase
RA 2,176,543 T→C 6.6% S196S (TCA→TCG yegX ← putative family 25 glycosyl hydrolase
RA 2,217,525 G→A 5.5% T275T (ACC→ACT pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,231,008 C→A 7.1% Q75H (CAG→CAT mglC ← methyl‑galactoside transporter subunit
RA 2,298,128 C→T 8.0% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,329,334 C→A 7.2% A264S (GCC→TCC)  yfaA ← DUF2138 family protein, putative host defense protein
RA 2,344,724 G→A 5.7% P377L (CCG→CTG)  glpT ← sn‑glycerol‑3‑phosphate transporter
RA 2,352,929 G→T 6.6% L235M (CTG→ATG)  rhmT ← putative L‑rhamnonate transporter
RA 2,363,545 C→A 6.4% R17S (CGT→AGT)  arnD → undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
RA 2,382,688 G→T 11.4% D32Y (GAT→TAT)  yfbP → TPR‑like repeats‑containing protein
RA 2,406,982 G→T 7.3% G12C (GGT→TGT)  ackA → acetate kinase A and propionate kinase 2
RA 2,465,527 C→T 6.4% pseudogene (75/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,532,009 C→A 5.7% intergenic (+167/+22) yfeS → / ← cysM WGR domain protein/cysteine synthase B (O‑acetylserine sulfhydrolase B)
RA 2,544,750 G→A 8.0% N112N (AAC→AAT yfeZ ← inner membrane protein
RA 2,548,112 G→A 5.3% L144F (CTT→TTT)  eutR ← eut operon transcriptional activator, AraC family
RA 2,559,397 C→A 5.5% G283C (GGC→TGC)  eutA ← reactivating factor for ethanolamine ammonia lyase
RA 2,561,558 A→G 5.7% intergenic (‑92/+125) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase, converts acetaldehyde to ethanol
RA 2,561,563 G→T 7.0% intergenic (‑97/+120) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase, converts acetaldehyde to ethanol
RA 2,574,761 C→A 6.9% D589E (GAC→GAA tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,644,122 C→A 7.2% V509L (GTG→TTG)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,650,475 G→A 5.8% A607V (GCG→GTG)  hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,650,497 T→C 5.3% M600V (ATG→GTG)  hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,694,897 C→A 9.8% T64T (ACG→ACT pdxJ ← pyridoxine 5'‑phosphate synthase
RA 2,707,703 C→T 7.6% intergenic (+114/+95) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,756,549 G→A 5.6% N117N (AAC→AAT yfjK ← DEAD/H helicase‑like protein, CP4‑57 putative defective prophage
RA 2,761,218 T→C 5.2% S50S (AGT→AGC yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,761,224 T→G 5.8% L52L (CTT→CTG yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,762,404 T→G 6.6% G127G (GGT→GGG yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,762,984 T→G 6.2% intergenic (+139/‑78) yfjQ → / → yfjR CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,803,255 G→T 5.3% G94C (GGC→TGC)  ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 2,811,408 T→A 11.1% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,812,752 C→T 5.3% A873A (GCG→GCA alaS ← alanyl‑tRNA synthetase
RA 2,838,796 G→T 5.6% T345T (ACC→ACA hycE ← hydrogenase 3, large subunit
RA 2,851,711 C→T 5.5% R420R (CGC→CGT mutS → methyl‑directed mismatch repair protein
RA 2,861,411 C→A 10.9% A234A (GCG→GCT nlpD ← activator of AmiC murein hydrolase activity, lipoprotein
RA 2,876,632 T→C 6.1% K289R (AAG→AGG)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 2,876,635 A→T 6.2% V288D (GTC→GAC)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 2,876,638 G→C 5.6% T287R (ACA→AGA)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 2,905,225 G→A 6.0% R596C (CGC→TGC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,929,313 C→T 7.4% A124V (GCT→GTT)  fucI → L‑fucose isomerase
RA 2,937,056 G→A 7.0% A121T (GCC→ACC)  csdA → cysteine sulfinate desulfinase
RA 2,958,661 G→A 5.9% R246C (CGC→TGC)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
RA 2,962,699 C→T 6.2% intergenic (‑363/‑322) rppH ← / → mutH RNA pyrophosphohydrolase/methyl‑directed mismatch repair protein
RA 2,963,688 C→T 6.7% A223V (GCC→GTC)  mutH → methyl‑directed mismatch repair protein
RA 2,970,855 G→A 9.3% A300T (GCC→ACC)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
RA 3,031,998 C→A 8.2% D62Y (GAT→TAT)  dsbC ← protein disulfide isomerase II
RA 3,046,394 C→A 6.8% D162Y (GAT→TAT)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
RA 3,058,429 G→A 5.3% G90D (GGT→GAT)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,063,427 A→C 9.6% intergenic (‑261/+97) mscS ← / ← fbaA mechanosensitive channel protein, small conductance/fructose‑bisphosphate aldolase, class II
RA 3,064,674 C→T 5.7% intergenic (‑71/+144) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,064,708 G→C 8.8% intergenic (‑105/+110) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,064,718 T→G 18.9% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,072,806 A→G 100% intergenic (‑117/+197) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
RA 3,108,596 C→T 5.2% P1292P (CCG→CCA yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,110,096 C→T 6.6% P792P (CCG→CCA yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,138,739 G→T 5.0% G294G (GGC→GGA hybO ← hydrogenase 2, small subunit
RA 3,145,031 C→T 7.0% E105K (GAA→AAA)  exbB ← membrane spanning protein in TonB‑ExbB‑ExbD complex
RA 3,151,338 G→T 7.3% V110V (GTG→GTT yqhG → DUF3828 family putative periplasmic protein
RA 3,221,494 G→T 20.9% S112I (AGC→ATC)  ygjJ → putative periplasmic protein
RA 3,242,676 C→A 6.6% L117M (CTG→ATG)  yqjC → DUF1090 family putative periplasmic protein
RA 3,255,041 C→T 5.3% G246D (GGC→GAC)  tdcE ← pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
RA 3,273,191 C→A 14.0% L307M (CTG→ATG)  kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit
RA 3,364,651 C→T 6.1% R428H (CGT→CAT)  nanT ← sialic acid transporter
RA 3,382,333 C→T 5.9% A51A (GCG→GCA aaeA ← p‑hydroxybenzoic acid efflux system component
RA 3,386,723 G→T 5.8% N965K (AAC→AAA yhdP ← DUF3971‑AsmA2 domains protein
RA 3,416,890 T→G 10.1% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 13.1% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,434,731 C→T 9.3% V102I (GTA→ATA)  rpsD ← 30S ribosomal subunit protein S4
RA 3,449,325 C→A 5.2% T130K (ACG→AAG)  gspC → general secretory pathway component, cryptic
RA 3,473,131 C→T 5.4% G279D (GGC→GAC)  kefB ← potassium:proton antiporter
RA 3,514,643 C→T 6.9% A56T (GCC→ACC)  hofO ← DNA catabolic protein
RA 3,542,745 G→C 5.4% Q229E (CAG→GAG)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,553,170 T→G 8.2% L515R (CTG→CGG)  rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,563,959 G→A 6.7% L241L (CTG→TTG)  glgX ← glycogen debranching enzyme
RA 3,599,873 C→A 8.0% T21T (ACC→ACA zntA → zinc, cobalt and lead efflux system
RA 3,614,441 G→T 7.0% G630G (GGG→GGT rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 10.8% G141G (GGA→GGG yhhI → putative transposase
RA 3,621,309 G→A 6.0% T530T (ACC→ACT rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,715,667 G→A 7.8% intergenic (+202/+21) sokA → / ← glyS pseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit
RA 3,755,089 G→T 12.3% A76D (GCT→GAT)  yibF ← glutathione S‑transferase homolog
RA 3,758,971 C→T 5.3% I1143I (ATC→ATT rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,780,627 C→T 6.6% R144C (CGT→TGT)  envC → activator of AmiB,C murein hydrolases, septal ring factor
RA 3,780,636 C→A 6.5% R147S (CGT→AGT)  envC → activator of AmiB,C murein hydrolases, septal ring factor
RA 3,813,650 G→A 7.5% P294L (CCG→CTG)  ligB ← DNA ligase, NAD(+)‑dependent
RA 3,862,560 A→C 7.6% L37R (CTG→CGG)  yidR ← DUF3748 family protein
RA 3,968,018 C→A 9.5% F64L (TTC→TTA wecD → TDP‑fucosamine acetyltransferase
RA 3,992,746 Δ1 bp 6.0% coding (1404/2163 nt) uvrD → DNA‑dependent ATPase I and helicase II
RA 3,992,749 G→T 6.5% A469A (GCG→GCT uvrD → DNA‑dependent ATPase I and helicase II
RA 3,992,751 Δ1 bp 6.0% coding (1409/2163 nt) uvrD → DNA‑dependent ATPase I and helicase II
RA 3,992,752 G→C 6.0% Q470H (CAG→CAC uvrD → DNA‑dependent ATPase I and helicase II
RA 3,992,754 A→T 6.0% E471V (GAA→GTA)  uvrD → DNA‑dependent ATPase I and helicase II
RA 4,006,947 G→T 7.2% D179Y (GAC→TAC)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,154,887 G→A 6.9% A441T (GCG→ACG)  btuB → vitamin B12/cobalamin outer membrane transporter
RA 4,156,331 A→G 17.1% intergenic (+118/‑256) murI → / → rrsB glutamate racemase/16S ribosomal RNA of rrnB operon
RA 4,161,576 A→C 9.2% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,162,053 C→T 5.3% A23A (GCC→GCT murB → UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
RA 4,163,192 T→C 6.6% P61P (CCT→CCC birA → bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase/ DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding
RA 4,165,449 C→T 16.1% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 16.1% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,173,745 G→A 20.0% G858D (GGT→GAT)  rpoB → RNA polymerase, beta subunit
RA 4,176,469 A→G 8.1% K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 55.1% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,185,954 G→A 5.4% T25I (ACC→ATC)  thiC ← phosphomethylpyrimidine synthase
RA 4,202,979 A→C 16.8% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,223,788 G→A 6.2% G35S (GGC→AGC)  pgi → glucosephosphate isomerase
RA 4,235,122 C→T 7.0% intergenic (‑119/+35) malF ← / ← malE maltose transporter subunit/maltose transporter subunit
RA 4,255,392 A→G 9.2% Q384R (CAG→CGG)  dnaB → replicative DNA helicase
RA 4,286,196 C→T 6.6% intergenic (+474/+57) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,297,526 G→A 8.7% H31Y (CAC→TAC)  alsK ← D‑allose kinase
RA 4,298,886 G→T 11.8% P134Q (CCG→CAG)  alsC ← D‑allose transporter subunit
RA 4,351,542 C→A 10.0% Q70H (CAG→CAT cadC ← cadBA operon transcriptional activator
RA 4,368,392 G→T 5.1% H124N (CAT→AAT)  ampC ← penicillin‑binding protein; beta‑lactamase, intrinsically weak
RA 4,368,905 G→A 5.5% H93H (CAC→CAT frdD ← fumarate reductase (anaerobic), membrane anchor subunit
RA 4,398,146 C→A 7.3% A559E (GCA→GAA)  rnr → exoribonuclease R, RNase R
RA 4,424,187 G→A 16.6% P86P (CCG→CCA ytfH → DUF24 family HxlR‑type putative transcriptional regulator
RA 4,428,332 C→T 6.6% A270V (GCG→GTG)  ytfI → uncharacterized protein
RA 4,483,882 G→A 8.9% G114G (GGC→GGT idnD ← L‑idonate 5‑dehydrogenase, NAD‑binding
RA 4,508,444 C→A 7.4% P34H (CCT→CAT) ‡ insA → IS1 repressor TnpA
RA 4,508,445 T→C 7.6% P34P (CCT→CCC) ‡ insA → IS1 repressor TnpA
RA 4,508,466 A→G 6.7% L41L (CTA→CTG insA → IS1 repressor TnpA
RA 4,508,660 T→G 5.8% pseudogene (123/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,750 G→T 6.6% pseudogene (213/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,897 T→A 9.0% pseudogene (360/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,554,134 C→G 8.2% G125R (GGC→CGC)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
RA 4,554,136 G→A 8.1% A124V (GCG→GTG)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
RA 4,561,269 G→A 78.3% S41S (AGC→AGT yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase
RA 4,562,725 G→T 10.0% pseudogene (495/1503 nt) yjiT → pseudogene
RA 4,598,741 C→A 5.1% I93I (ATC→ATA yjjG → dUMP phosphatase
RA 4,604,397 G→A 7.0% intergenic (+320/+100) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,628,905 C→T 7.6% A304V (GCA→GTA)  creD → inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 3448752 3448865 114 13 [9] [3] 13 [gspA] [gspA]
* * ÷ CP009273 3745284 3745346 63 12 [9] [10] 12 yiaT/yiaU putative outer membrane protein/putative DNA‑binding transcriptional regulator
* * ÷ CP009273 3763025 3763249 225 12 [9] [11] 12 [yibV]–yibV [yibV],yibV
* * ÷ CP009273 4323148 4323774 627 20 [3] [11] 12 [basR]–[eptA] [basR],[eptA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 25796NA (NA)6 (0.100) 4/246 NT NA intergenic (+95/‑30) lspA/fkpB prolipoprotein signal peptidase (signal peptidase II)/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
?CP009273 372778 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =44 (0.710)58 (0.940) 26/248 NT 56.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370378 =NA (NA)14 (0.240) 5/232 NT NA intergenic (+41/‑537) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 372785 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 370386 =NA (NA)16 (0.370) 8/174 NT 16.1% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372878 = 91 (1.920)noncoding (88/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 1203246 =30 (0.480)33 (0.610) 23/216 NT 54.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 28 (0.520)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326128 (0.450)18 (0.330) 10/216 NT 40.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 28 (0.520)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related