breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 104,041 G→T 15.1% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
RA 148,133 A→T 5.1% F196I (TTT→ATT)  yadL ← putative fimbrial‑like adhesin protein
RA 161,068 A→C 27.2% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 235,019 C→A 5.6% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 353,335 T→G 6.6% intergenic (+170/+167) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 418,994 C→G 9.8% L341V (CTG→GTG)  malZ → maltodextrin glucosidase
RA 445,212 C→T 100% G296S (GGT→AGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 1,160,015 A→C 8.8% E155D (GAA→GAC nagZ → beta N‑acetyl‑glucosaminidase
RA 1,207,963 C→A 6.2% intergenic (‑504/+196) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
RA 1,288,276 C→A 100% V35F (GTT→TTT)  hns ← global DNA‑binding transcriptional dual regulator H‑NS
RA 1,896,745 A→C 10.7% K147N (AAA→AAC manX → fused mannose‑specific PTS enzymes: IIA component/IIB component
RA 2,170,713 C→A 11.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,724 G→T 14.6% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,758 C→A 9.1% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,632,621 T→G 11.1% N8H (AAC→CAC)  yfgM ← putative anti‑RcsB factor
RA 2,632,626 T→G 6.6% N6T (AAC→ACC)  yfgM ← putative anti‑RcsB factor
RA 2,632,650 T→G 7.3% intergenic (‑8/+10) yfgM ← / ← hisS putative anti‑RcsB factor/histidyl tRNA synthetase
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,958,490 G→T 5.8% Q9K (CAA→AAA)  thyA ← thymidylate synthetase
RA 3,072,806 A→G 100% intergenic (‑117/+197) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
RA 3,232,977 A→T 9.2% intergenic (+73/‑326) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,417,135 G→T 9.0% noncoding (12/120 nt) rrfD ← 5S ribosomal RNA of rrnD operon
RA 3,602,063 A→T 12.8% intergenic (+54/+48) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,602,066 G→T 7.4% intergenic (+57/+45) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,822,340 A→C 8.9% Q12H (CAA→CAC xanP → xanthine permease
RA 3,901,867 T→G 9.9% intergenic (‑141/+42) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 3,901,880 A→C 6.8% intergenic (‑154/+29) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 4,173,025 A→T 9.7% Q618L (CAG→CTG)  rpoB → RNA polymerase, beta subunit
JC 4,173,705 Δ9 bp 7.0% coding (2533‑2541/4029 nt) rpoB → RNA polymerase, beta subunit
RA 4,174,687 T→G 21.7% L1172R (CTG→CGG)  rpoB → RNA polymerase, beta subunit
RA 4,176,502 T→G 6.2% W409G (TGG→GGG)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,209 C→T 26.9% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,501 G→C 11.8% R1075P (CGT→CCT)  rpoC → RNA polymerase, beta prime subunit
RA 4,234,581 G→T 6.7% G141G (GGC→GGA malF ← maltose transporter subunit
RA 4,236,457 T→G 7.4% intergenic (‑110/‑255) malE ← / → malK maltose transporter subunit/fused maltose transport subunit, ATP‑binding component of ABC superfamily/regulatory protein
RA 4,286,118 A→G 5.2% intergenic (+396/+135) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,286,119 A→C 6.7% intergenic (+397/+134) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,375,469 A→C 8.4% Y87S (TAC→TCC)  yjeN → uncharacterized protein
RA 4,561,269 G→A 100% S41S (AGC→AGT yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 4323062 4323797 736 106 [98] [99] 102 [basS]–[eptA] [basS],basR,[eptA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =287 (0.980)319 (1.090) 76/248 NT 52.6% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =150 (0.510)187 (0.740) 55/216 NT 62.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 99 (0.390)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1203261147 (0.500)176 (0.690) 53/216 NT 60.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 99 (0.390)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 4532499 =72 (0.250)14 (0.050) 7/230 NT 10.9% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 161 (0.600)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)