breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 68,486 T→G 13.9% L217V (TTG→GTG)  yabI → DedA family inner membrane protein
RA 161,068 A→C 24.7% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 221,420 A→G 5.1% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 5.3% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 5.4% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 6.2% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 357,060 T→G 9.8% N171T (AAC→ACC)  lacA ← thiogalactoside acetyltransferase
RA 418,995 T→G 14.1% L341R (CTG→CGG)  malZ → maltodextrin glucosidase
RA 445,212 C→T 100% G296S (GGT→AGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 838,652 T→G 12.8% F289V (TTT→GTT)  rlmF → 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
RA 910,671 T→G 8.3% intergenic (‑358/+137) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 1,288,276 C→A 100% V35F (GTT→TTT)  hns ← global DNA‑binding transcriptional dual regulator H‑NS
RA 1,320,988 T→G 9.0% intergenic (+90/‑121) yciQ → / → rluB enhancer of membrane protein expression; putative inner membrane protein/23S rRNA pseudouridine(2605) synthase
RA 1,364,326 A→C 6.9% intergenic (+66/‑147) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
RA 2,170,724 G→T 16.0% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,298,128 C→T 6.2% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,707,492 A→C 5.6% K413T (AAG→ACG)  srmB → ATP‑dependent RNA helicase
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,832,598 T→G 5.3% K9T (AAG→ACG)  ascG ← asc operon transcriptional repressor; prpBC operon repressor
RA 3,072,806 A→G 100% intergenic (‑117/+197) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
RA 3,119,692 T→G 5.8% T64P (ACC→CCC)  glcE ← glycolate oxidase FAD binding subunit
RA 3,525,180 T→G 5.1% I207L (ATC→CTC)  yhgE ← DUF4153 family putative inner membrane protein
RA 3,525,235 C→A 5.5% T188T (ACG→ACT yhgE ← DUF4153 family putative inner membrane protein
RA 3,822,340 A→C 9.9% Q12H (CAA→CAC xanP → xanthine permease
RA 3,901,867 T→G 5.8% intergenic (‑141/+42) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 3,991,416 T→G 9.8% L25R (CTT→CGT)  uvrD → DNA‑dependent ATPase I and helicase II
RA 4,173,307 C→T 8.5% S712F (TCC→TTC)  rpoB → RNA polymerase, beta subunit
RA 4,177,142 A→G 9.0% D622G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,209 C→T 37.7% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,501 G→T 21.8% R1075L (CGT→CTT)  rpoC → RNA polymerase, beta prime subunit
JC 4,178,889 (GAACGTGTA)1→2 16.9% coding (3612/4224 nt) rpoC → RNA polymerase, beta prime subunit
RA 4,561,269 G→A 100% S41S (AGC→AGT yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 4323073 4323787 715 86 [83] [83] 87 [basS]–[eptA] [basS],basR,[eptA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =244 (0.940)319 (1.230) 70/248 NT 56.7% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =115 (0.440)156 (0.690) 54/216 NT 67.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 48 (0.210)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1203261108 (0.410)153 (0.680) 46/216 NT 68.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 48 (0.210)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related