breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 117 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,602,0102.C74.1% 63.9 / 21.1 27coding (193/2019 nt)hyfBhydrogenase 4 component B
*NC_0009131,097,2190AG63.2% ‑2.9 / 14.8 20intergenic (+390/+346)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009131,097,1090AT56.2% ‑3.1 / 10.4 17intergenic (+280/+456)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_000913707,9760TG54.5% ‑1.6 / 24.7 33intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913497,5850CA51.8% 5.7 / 19.4 28F137L (TTC→TTAadkadenylate kinase
*NC_000913454,9060GT50.0% 14.5 / 13.7 49intergenic (+117/‑227)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_000913454,9260GC50.0% 18.8 / 17.2 46intergenic (+137/‑207)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,177,2450GT50.0% ‑0.1 / 28.5 54intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4270CA50.0% 38.8 / 12.3 47Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009133,455,6710AT50.0% 12.3 / 26.0 44I32F (ATT→TTT) gspCType II secretion system protein GspC
*NC_0009133,455,6800GT48.8% 18.8 / 23.4 43V35L (GTG→TTG) gspCType II secretion system protein GspC
*NC_0009131,636,4420CG48.3% 21.4 / 11.0 29intergenic (‑75/‑314)nohA/ynfOQin prophage; putative prophage DNA‑packaging protein NohA/Qin prophage; protein YnfO
*NC_0009132,177,2340CA47.2% 23.3 / 13.7 53intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9840CG47.1% 7.9 / 18.3 34intergenic (+227/‑350)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913729,3720AC46.9% 5.9 / 11.9 33intergenic (+32/‑211)ybfA/rhsCDUF2517 domain‑containing protein YbfA/rhs element protein RhsC
*NC_0009131,879,8460TG46.8% 31.9 / 26.9 47T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
*NC_0009134,378,9730GC46.2% ‑0.2 / 21.3 39intergenic (‑29/+34)ampC/frdDbeta‑lactamase/fumarate reductase membrane protein FrdD
*NC_000913707,9810CT45.5% 6.0 / 20.9 34intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,470,4360CA45.5% 38.1 / 10.1 44Q436K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009133,004,9460GT45.1% 38.9 / 36.2 71Q281K (CAA→AAA) ygeVputative sigma(54)‑dependent transcriptional regulator YgeV

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2290113NA (NA)29 (0.380) 12/256 3.9 30.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4118284 = 67 (0.870)intergenic (‑194/‑231) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 = 120780563 (0.820)13 (0.190) 10/224 3.9 18.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 62 (0.920)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 2288919 =NA (NA)29 (0.380) 8/256 5.1 28.7% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4118287 72 (0.940)intergenic (‑197/‑228) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 = 2067353NA (NA)11 (0.140) 8/256 5.1 14.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4118284 = 67 (0.870)intergenic (‑194/‑231) glpF/zapB glycerol facilitator/cell division factor ZapB
* ? NC_000913 = 454269057 (0.740)4 (0.060) 4/238 6.5 5.5% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 85 (1.190)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 1467910 =NA (NA)6 (0.080) 4/256 6.8 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 1469241 NA (NA)intergenic (‑92/‑5) insC‑2/ydbA IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment
* ? NC_000913 = 329741535 (0.460)3 (0.040) 3/250 7.3 6.2% coding (724/1041 nt) yraQ permease family protein YraQ
?NC_000913 3987717 = 56 (0.750)coding (166/1197 nt) hemY protein HemY
* ? NC_000913 2068944 =NA (NA)5 (0.070) 3/250 7.3 NA noncoding (1328/1331 nt) IS2 repeat region
?NC_000913 = 2070275 NA (NA)intergenic (‑95/‑2) insC‑3/yoeA CP4‑44 prophage; IS2 insertion element repressor InsA/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
* ? NC_000913 1467910 =NA (NA)3 (0.040) 3/256 7.4 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 1469242 NA (NA)intergenic (‑93/‑4) insC‑2/ydbA IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment