breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 4,177,543 | 0 | G | A | 45.6% | 42.0 / 210.0 | 138 | E756K (GAA→AAA) | rpoC | RNA polymerase, beta prime subunit |
* | CP009273 | 2,170,734 | 0 | G | T | 44.8% | 23.0 / 97.6 | 134 | intergenic (‑51/+257) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I |
* | CP009273 | 4,402,598 | 0 | C | G | 30.6% | 162.8 / 28.0 | 87 | S132C (TCT→TGT) | yjfM | DUF1190 family protein |
* | CP009273 | 2,998,305 | 0 | G | T | 26.6% | 155.5 / 25.1 | 113 | Q281K (CAA→AAA) | ygeV | putative sigma‑54‑interacting transcriptional activator |
* | CP009273 | 838,659 | 0 | C | G | 26.0% | 219.9 / 21.0 | 104 | A291G (GCC→GGC) ‡ | rlmF | 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent |
* | CP009273 | 4,178,209 | 0 | C | T | 25.7% | 196.7 / 73.8 | 101 | R978C (CGT→TGT) | rpoC | RNA polymerase, beta prime subunit |
* | CP009273 | 4,402,596 | 0 | T | G | 25.3% | 177.1 / 10.6 | 87 | V131V (GTT→GTG) | yjfM | DUF1190 family protein |
* | CP009273 | 4,402,656 | 0 | C | A | 24.6% | 115.8 / 21.8 | 66 | N151K (AAC→AAA) | yjfM | DUF1190 family protein |
* | CP009273 | 2,170,713 | 0 | C | A | 24.2% | 101.3 / 13.3 | 104 | intergenic (‑30/+278) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I |
* | CP009273 | 4,402,608 | 0 | G | T | 24.2% | 142.2 / 25.7 | 91 | L135F (TTG→TTT) | yjfM | DUF1190 family protein |
* | CP009273 | 838,658 | 0 | G | T | 24.0% | 189.1 / 17.2 | 104 | A291S (GCC→TCC) ‡ | rlmF | 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent |
* | CP009273 | 2,998,333 | 0 | T | A | 23.2% | 273.6 / 23.0 | 115 | Q271H (CAA→CAT) | ygeV | putative sigma‑54‑interacting transcriptional activator |
* | CP009273 | 838,652 | 0 | T | G | 22.2% | 222.1 / 10.1 | 108 | F289V (TTT→GTT) | rlmF | 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent |
* | CP009273 | 3,530,686 | 0 | G | T | 20.2% | 137.7 / 12.0 | 79 | intergenic (+39/‑58) | greB/yhgF | transcript cleavage factor/putative transcriptional accessory protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 2550547 = | 131 (1.040) | 32 (0.260) | 20/260 | 3.1 | 21.3% | coding (110/888 nt) | eutC | ethanolamine ammonia‑lyase, small subunit (light chain) |
? | CP009273 | = 2550563 | 110 (0.900) | coding (94/888 nt) | eutC | ethanolamine ammonia‑lyase, small subunit (light chain) | |||||
* | ? | CP009273 | = 4532507 | 54 (0.430) | 21 (0.180) | 11/250 | 5.1 | 23.8% | intergenic (+57/‑425) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | CP009273 | = 4532803 | 84 (0.720) | intergenic (+353/‑129) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) | |||||
* | ? | CP009273 | = 2811716 | 134 (1.070) | 7 (0.070) | 3/228 | 8.3 | 6.7% | intergenic (‑83/+116) | argY/argV | tRNA‑Arg/tRNA‑Arg |
? | CP009273 | 2811851 = | 81 (0.760) | noncoding (58/77 nt) | argV | tRNA‑Arg | |||||
* | ? | CP009273 | 3399761 = | 175 (1.390) | 3 (0.030) | 3/244 | 8.6 | 1.8% | coding (486/1350 nt) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
? | CP009273 | = 3399757 | 169 (1.480) | coding (482/1350 nt) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit | |||||
* | ? | CP009273 | 1884963 = | 94 (0.750) | 3 (0.020) | 3/264 | 9.1 | 2.3% | coding (562/696 nt) | tsaB | tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; binding partner and protease for TsaD |
? | CP009273 | 2294202 = | 166 (1.340) | coding (2031/2487 nt) | napA | nitrate reductase, periplasmic, large subunit | |||||
* | ? | CP009273 | 1249900 = | 108 (0.860) | 3 (0.020) | 3/264 | 9.1 | 3.0% | coding (1509/2868 nt) | ycgV | putative adhesin |
? | CP009273 | = 1393926 | 87 (0.700) | intergenic (‑143/+52) | fnr/ogt | oxygen‑sensing anaerobic growth regulon transcriptional regulator; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase | |||||
* | ? | CP009273 | 3851510 = | 109 (0.870) | 3 (0.020) | 3/268 | 9.2 | 2.3% | coding (255/1494 nt) | yidJ | sulfatase/phosphatase superfamily protein |
? | CP009273 | = 3851661 | 145 (1.150) | coding (104/1494 nt) | yidJ | sulfatase/phosphatase superfamily protein |