breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 80 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 707,976 | 0 | T | G | 64.1% | 2.1 / 21.8 | 39 | intergenic (+219/‑358) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 454,919 | 0 | G | T | 61.9% | ‑1.2 / 11.5 | 24 | intergenic (+130/‑214) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 4,283,226 | 0 | G | C | 61.5% | 3.5 / 27.7 | 26 | intergenic (‑151/+27) | yjcF/actP | pentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter |
* | NC_000913 | 2,470,427 | 0 | C | A | 60.0% | 5.4 / 26.3 | 32 | Q433K (CAA→AAA) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 2,177,234 | 0 | C | A | 59.1% | 0.1 / 10.0 | 32 | intergenic (‑30/+278) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,470,436 | 0 | C | A | 56.7% | 7.1 / 25.7 | 30 | Q436K (CAA→AAA) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 2,177,245 | 0 | G | T | 54.8% | ‑2.2 / 15.6 | 34 | intergenic (‑41/+267) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,177,255 | 0 | G | T | 54.0% | ‑6.1 / 30.7 | 38 | intergenic (‑51/+257) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 707,981 | 0 | C | G | 52.2% | ‑6.3 / 11.5 | 39 | intergenic (+224/‑353) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 1,636,442 | 0 | C | G | 51.7% | 8.3 / 31.4 | 30 | intergenic (‑75/‑314) | nohA/ynfO | Qin prophage; putative prophage DNA‑packaging protein NohA/Qin prophage; protein YnfO |
* | NC_000913 | 708,004 | 0 | C | A | 50.0% | 31.3 / 14.4 | 41 | intergenic (+247/‑330) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 3,455,671 | 0 | A | T | 50.0% | 16.9 / 23.0 | 36 | I32F (ATT→TTT) | gspC | Type II secretion system protein GspC |
* | NC_000913 | 3,455,677 | 0 | A | T | 50.0% | 12.7 / 22.4 | 34 | N34Y (AAT→TAT) | gspC | Type II secretion system protein GspC |
* | NC_000913 | 2,177,228 | 0 | C | T | 47.6% | 0.0 / 20.5 | 32 | intergenic (‑24/+284) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 707,984 | 0 | C | G | 47.4% | 14.5 / 19.8 | 39 | intergenic (+227/‑350) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 2,470,411 | 0 | A | T | 45.2% | 19.1 / 17.4 | 31 | L427F (TTA→TTT) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 2,570,475 | 0 | A | C | 42.9% | 27.3 / 11.3 | 28 | L426R (CTG→CGG) | eutE | putative aldehyde dehydrogenase, ethanolamine utilization protein |
* | NC_000913 | 1,982,973 | 0 | G | C | 41.9% | 36.4 / 10.9 | 31 | L190V (CTG→GTG) | araH | arabinose ABC transporter membrane subunit |
* | NC_000913 | 3,455,680 | 0 | G | T | 41.9% | 28.1 / 10.1 | 32 | V35L (GTG→TTG) | gspC | Type II secretion system protein GspC |
* | NC_000913 | 2,470,405 | 0 | T | G | 41.4% | 24.0 / 12.8 | 29 | V425V (GTT→GTG) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4498181 = | NA (NA) | 9 (0.140) | 6/254 | 4.8 | NA | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 4499512 | NA (NA) | intergenic (+12/+81) | insD‑6/yjgX | KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment | |||||
* | ? | NC_000913 | 381258 = | NA (NA) | 11 (0.170) | 6/252 | 4.8 | NA | pseudogene (3/417 nt) | yaiX | putative acyltransferase, N‑terminal fragment |
? | NC_000913 | = 382589 | NA (NA) | noncoding (1330/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 4498180 = | NA (NA) | 20 (0.310) | 5/254 | 5.3 | NA | intergenic (+240/‑92) | intB/insC‑6 | KpLE2 phage‑like element; putative integrase/KpLE2 phage‑like element; IS2 insertion element repressor InsA |
? | NC_000913 | = 4499511 | NA (NA) | noncoding (1331/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 1467909 = | NA (NA) | 12 (0.190) | 5/254 | 5.3 | NA | pseudogene (2518/2518 nt) | ydbA | putative outer membrane protein, N‑terminal fragment |
? | NC_000913 | = 1469240 | NA (NA) | noncoding (1/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 1467910 = | NA (NA) | 4 (0.060) | 4/254 | 5.7 | NA | noncoding (1331/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 1469242 | NA (NA) | intergenic (‑93/‑4) | insC‑2/ydbA | IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment |