breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 80 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913707,9760TG64.1% 2.1 / 21.8 39intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913454,9190GT61.9% ‑1.2 / 11.5 24intergenic (+130/‑214)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009134,283,2260GC61.5% 3.5 / 27.7 26intergenic (‑151/+27)yjcF/actPpentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
*NC_0009132,470,4270CA60.0% 5.4 / 26.3 32Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2340CA59.1% 0.1 / 10.0 32intergenic (‑30/+278)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,470,4360CA56.7% 7.1 / 25.7 30Q436K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2450GT54.8% ‑2.2 / 15.6 34intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2550GT54.0% ‑6.1 / 30.7 38intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9810CG52.2% ‑6.3 / 11.5 39intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009131,636,4420CG51.7% 8.3 / 31.4 30intergenic (‑75/‑314)nohA/ynfOQin prophage; putative prophage DNA‑packaging protein NohA/Qin prophage; protein YnfO
*NC_000913708,0040CA50.0% 31.3 / 14.4 41intergenic (+247/‑330)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009133,455,6710AT50.0% 16.9 / 23.0 36I32F (ATT→TTT) gspCType II secretion system protein GspC
*NC_0009133,455,6770AT50.0% 12.7 / 22.4 34N34Y (AAT→TAT) gspCType II secretion system protein GspC
*NC_0009132,177,2280CT47.6% 0.0 / 20.5 32intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9840CG47.4% 14.5 / 19.8 39intergenic (+227/‑350)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,470,4110AT45.2% 19.1 / 17.4 31L427F (TTA→TTTyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,570,4750AC42.9% 27.3 / 11.3 28L426R (CTG→CGG) eutEputative aldehyde dehydrogenase, ethanolamine utilization protein
*NC_0009131,982,9730GC41.9% 36.4 / 10.9 31L190V (CTG→GTG) araHarabinose ABC transporter membrane subunit
*NC_0009133,455,6800GT41.9% 28.1 / 10.1 32V35L (GTG→TTG) gspCType II secretion system protein GspC
*NC_0009132,470,4050TG41.4% 24.0 / 12.8 29V425V (GTT→GTGyfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4498181 =NA (NA)9 (0.140) 6/254 4.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 381258 =NA (NA)11 (0.170) 6/252 4.8 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 4498180 =NA (NA)20 (0.310) 5/254 5.3 NA intergenic (+240/‑92) intB/insC‑6 KpLE2 phage‑like element; putative integrase/KpLE2 phage‑like element; IS2 insertion element repressor InsA
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 1467909 =NA (NA)12 (0.190) 5/254 5.3 NA pseudogene (2518/2518 nt) ydbA putative outer membrane protein, N‑terminal fragment
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1467910 =NA (NA)4 (0.060) 4/254 5.7 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 1469242 NA (NA)intergenic (‑93/‑4) insC‑2/ydbA IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment