breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 123 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,470,427 | 0 | C | A | 61.9% | 10.8 / 42.5 | 43 | Q433K (CAA→AAA) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 2,177,245 | 0 | G | T | 61.2% | 11.8 / 16.9 | 54 | intergenic (‑41/+267) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 707,976 | 0 | T | G | 61.1% | ‑6.0 / 24.2 | 36 | intergenic (+219/‑358) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 2,470,436 | 0 | C | A | 57.1% | 12.9 / 43.6 | 42 | Q436K (CAA→AAA) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 2,177,255 | 0 | G | T | 53.4% | 6.2 / 34.9 | 58 | intergenic (‑51/+257) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 2,177,221 | 0 | T | G | 52.6% | 7.3 / 51.7 | 57 | intergenic (‑17/+291) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
* | NC_000913 | 4,283,226 | 0 | G | C | 52.5% | 26.3 / 56.1 | 40 | intergenic (‑151/+27) | yjcF/actP | pentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter |
* | NC_000913 | 4,082,697 | 0 | A | C | 52.4% | ‑4.0 / 11.1 | 27 | P21P (CCT→CCG) | fdoH | formate dehydrogenase O subunit beta |
* | NC_000913 | 2,449,200 | 0 | G | T | 52.2% | 6.9 / 16.2 | 24 | intergenic (+43/+28) | smrB/yfcO | putative endonuclease SmrB/DUF2544 domain‑containing protein YfcO |
* | NC_000913 | 454,866 | 0 | G | T | 47.6% | ‑3.6 / 10.1 | 21 | intergenic (+77/‑267) | bolA/tig | DNA‑binding transcriptional dual regulator BolA/trigger factor |
* | NC_000913 | 2,139,598 | 0 | T | G | 44.8% | 36.1 / 17.4 | 67 | intergenic (+113/+161) | yegH/asmA | inner membrane protein YegH/putative assembly protein AsmA |
* | NC_000913 | 707,984 | 0 | C | G | 44.7% | 32.2 / 13.9 | 39 | intergenic (+227/‑350) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 1,879,846 | 0 | T | G | 44.1% | 51.8 / 26.2 | 59 | T35P (ACG→CCG) | yeaR | DUF1971 domain‑containing protein YeaR |
* | NC_000913 | 707,981 | 0 | C | G | 44.0% | 12.7 / 17.7 | 38 | intergenic (+224/‑353) | glnS/chiP | glutamine‑‑tRNA ligase/chitobiose outer membrane channel |
* | NC_000913 | 729,369 | 0 | G | C | 43.2% | 1.9 / 14.7 | 37 | intergenic (+29/‑214) | ybfA/rhsC | DUF2517 domain‑containing protein YbfA/rhs element protein RhsC |
* | NC_000913 | 1,708,326 | 0 | G | T | 42.4% | 77.4 / 22.0 | 67 | E470* (GAA→TAA) | rsxC | SoxR [2Fe‑2S] reducing system protein RsxC |
* | NC_000913 | 729,688 | 0 | T | G | 41.9% | 73.0 / 17.2 | 62 | C36G (TGT→GGT) | rhsC | rhs element protein RhsC |
* | NC_000913 | 2,470,416 | 0 | A | T | 41.7% | 37.3 / 19.5 | 48 | Y429F (TAT→TTT) | yfdI | CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase |
* | NC_000913 | 238,535 | 0 | G | T | 40.0% | 87.0 / 16.9 | 81 | H68N (CAT→AAT) | ykfM | uncharacterized protein YkfM |
* | NC_000913 | 1,326,937 | 0 | T | G | 40.0% | 41.9 / 11.4 | 40 | V29G (GTG→GGG) | rluB | 23S rRNA pseudouridine(2605) synthase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4498181 = | NA (NA) | 13 (0.150) | 9/258 | 5.4 | NA | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 4499512 | NA (NA) | intergenic (+12/+81) | insD‑6/yjgX | KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment | |||||
* | ? | NC_000913 | 381258 = | NA (NA) | 13 (0.150) | 9/256 | 5.4 | NA | pseudogene (3/417 nt) | yaiX | putative acyltransferase, N‑terminal fragment |
? | NC_000913 | = 382589 | NA (NA) | noncoding (1330/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | 1467910 = | NA (NA) | 7 (0.080) | 7/258 | 6.2 | NA | noncoding (1331/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 1469242 | NA (NA) | intergenic (‑93/‑4) | insC‑2/ydbA | IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment | |||||
* | ? | NC_000913 | = 1670944 | 94 (1.090) | 4 (0.050) | 3/258 | 8.2 | 4.0% | coding (1246/1254 nt) | ynfM | putative transporter YnfM |
? | NC_000913 | 1670948 = | 96 (1.110) | coding (1250/1254 nt) | ynfM | putative transporter YnfM | |||||
* | ? | NC_000913 | = 1249069 | 85 (0.990) | 3 (0.040) | 3/256 | 8.2 | 3.2% | coding (46/1419 nt) | dhaM | dihydroxyacetone kinase subunit M |
? | NC_000913 | = 4377054 | 99 (1.160) | coding (180/318 nt) | gdx | guanidinium exporter |