breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 123 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA61.9% 10.8 / 42.5 43Q433K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2450GT61.2% 11.8 / 16.9 54intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913707,9760TG61.1% ‑6.0 / 24.2 36intergenic (+219/‑358)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009132,470,4360CA57.1% 12.9 / 43.6 42Q436K (CAA→AAA) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_0009132,177,2550GT53.4% 6.2 / 34.9 58intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009132,177,2210TG52.6% 7.3 / 51.7 57intergenic (‑17/+291)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009134,283,2260GC52.5% 26.3 / 56.1 40intergenic (‑151/+27)yjcF/actPpentapeptide repeat‑containing protein YjcF/acetate/glycolate:cation symporter
*NC_0009134,082,6970AC52.4% ‑4.0 / 11.1 27P21P (CCT→CCGfdoHformate dehydrogenase O subunit beta
*NC_0009132,449,2000GT52.2% 6.9 / 16.2 24intergenic (+43/+28)smrB/yfcOputative endonuclease SmrB/DUF2544 domain‑containing protein YfcO
*NC_000913454,8660GT47.6% ‑3.6 / 10.1 21intergenic (+77/‑267)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009132,139,5980TG44.8% 36.1 / 17.4 67intergenic (+113/+161)yegH/asmAinner membrane protein YegH/putative assembly protein AsmA
*NC_000913707,9840CG44.7% 32.2 / 13.9 39intergenic (+227/‑350)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_0009131,879,8460TG44.1% 51.8 / 26.2 59T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
*NC_000913707,9810CG44.0% 12.7 / 17.7 38intergenic (+224/‑353)glnS/chiPglutamine‑‑tRNA ligase/chitobiose outer membrane channel
*NC_000913729,3690GC43.2% 1.9 / 14.7 37intergenic (+29/‑214)ybfA/rhsCDUF2517 domain‑containing protein YbfA/rhs element protein RhsC
*NC_0009131,708,3260GT42.4% 77.4 / 22.0 67E470* (GAA→TAA) rsxCSoxR [2Fe‑2S] reducing system protein RsxC
*NC_000913729,6880TG41.9% 73.0 / 17.2 62C36G (TGT→GGT) rhsCrhs element protein RhsC
*NC_0009132,470,4160AT41.7% 37.3 / 19.5 48Y429F (TAT→TTT) yfdICPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
*NC_000913238,5350GT40.0% 87.0 / 16.9 81H68N (CAT→AAT) ykfMuncharacterized protein YkfM
*NC_0009131,326,9370TG40.0% 41.9 / 11.4 40V29G (GTG→GGG) rluB23S rRNA pseudouridine(2605) synthase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4498181 =NA (NA)13 (0.150) 9/258 5.4 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 381258 =NA (NA)13 (0.150) 9/256 5.4 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 1467910 =NA (NA)7 (0.080) 7/258 6.2 NA noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 1469242 NA (NA)intergenic (‑93/‑4) insC‑2/ydbA IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment
* ? NC_000913 = 167094494 (1.090)4 (0.050) 3/258 8.2 4.0% coding (1246/1254 nt) ynfM putative transporter YnfM
?NC_000913 1670948 = 96 (1.110)coding (1250/1254 nt) ynfM putative transporter YnfM
* ? NC_000913 = 124906985 (0.990)3 (0.040) 3/256 8.2 3.2% coding (46/1419 nt) dhaM dihydroxyacetone kinase subunit M
?NC_000913 = 4377054 99 (1.160)coding (180/318 nt) gdx guanidinium exporter