mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L001_R1_001.good.fq | 326,799 | 42,078,792 | 100.0% | 128.8 bases | 137 bases | 97.5% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L001_R2_001.good.fq | 326,799 | 42,079,953 | 100.0% | 128.8 bases | 137 bases | 95.6% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L002_R1_001.good.fq | 327,370 | 42,176,335 | 100.0% | 128.8 bases | 137 bases | 98.2% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L002_R2_001.good.fq | 327,370 | 42,178,063 | 100.0% | 128.8 bases | 137 bases | 96.8% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L003_R1_001.good.fq | 325,456 | 41,836,473 | 100.0% | 128.5 bases | 137 bases | 98.2% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L003_R2_001.good.fq | 325,456 | 41,836,178 | 100.0% | 128.5 bases | 137 bases | 97.0% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L004_R1_001.good.fq | 333,825 | 42,925,571 | 100.0% | 128.6 bases | 137 bases | 98.4% |
| errors | TF_KALE_lrp_A21_F89_I5_R1_S31_L004_R2_001.good.fq | 333,825 | 42,925,734 | 100.0% | 128.6 bases | 137 bases | 97.5% |
| total | 2,626,900 | 338,037,099 | 100.0% | 128.7 bases | 137 bases | 97.4% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 70.2 | 3.9 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 1976 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 163 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.010 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.83892 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 21:20:30 22 Apr 2021 | 21:21:14 22 Apr 2021 | 44 seconds |
| Read alignment to reference genome | 21:21:14 22 Apr 2021 | 21:26:38 22 Apr 2021 | 5 minutes 24 seconds |
| Preprocessing alignments for candidate junction identification | 21:26:38 22 Apr 2021 | 21:27:09 22 Apr 2021 | 31 seconds |
| Preliminary analysis of coverage distribution | 21:27:09 22 Apr 2021 | 21:28:38 22 Apr 2021 | 1 minute 29 seconds |
| Identifying junction candidates | 21:28:38 22 Apr 2021 | 21:28:39 22 Apr 2021 | 1 second |
| Re-alignment to junction candidates | 21:28:39 22 Apr 2021 | 21:29:58 22 Apr 2021 | 1 minute 19 seconds |
| Resolving best read alignments | 21:29:58 22 Apr 2021 | 21:30:43 22 Apr 2021 | 45 seconds |
| Creating BAM files | 21:30:43 22 Apr 2021 | 21:32:04 22 Apr 2021 | 1 minute 21 seconds |
| Tabulating error counts | 21:32:04 22 Apr 2021 | 21:32:34 22 Apr 2021 | 30 seconds |
| Re-calibrating base error rates | 21:32:34 22 Apr 2021 | 21:32:37 22 Apr 2021 | 3 seconds |
| Examining read alignment evidence | 21:32:37 22 Apr 2021 | 21:38:50 22 Apr 2021 | 6 minutes 13 seconds |
| Polymorphism statistics | 21:38:50 22 Apr 2021 | 21:38:50 22 Apr 2021 | 0 seconds |
| Output | 21:38:50 22 Apr 2021 | 21:38:59 22 Apr 2021 | 9 seconds |
| Total | 18 minutes 29 seconds | ||
