breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,177,5860TC74.5% 426.5 / 163.4 213L770P (CTC→CCC) rpoCRNA polymerase, beta prime subunit
*CP0092734,178,3390AC64.8% 207.7 / 214.5 202D1021A (GAC→GCC) rpoCRNA polymerase, beta prime subunit
*CP0092731,314,7680CT53.4% 21.0 / 156.0 103A292T (GCC→ACC) trpDfused glutamine amidotransferase (component II) of anthranilate synthase/anthranilate phosphoribosyl transferase
*CP0092734,178,5000CT30.6% 310.6 / 183.1 196R1075C (CGT→TGT) rpoCRNA polymerase, beta prime subunit
*CP0092732,170,7340GT30.6% 183.1 / 74.7 193intergenic (‑51/+257)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP009273104,0420AT28.2% 249.2 / 35.6 105intergenic (+81/‑150)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP009273357,0310CA27.7% 189.2 / 34.2 112V181F (GTT→TTT) lacAthiogalactoside acetyltransferase
*CP0092732,475,2990CA26.7% 125.9 / 10.7 75M119I (ATG→ATTemrYputative multidrug efflux system
*CP0092732,998,3140CA25.2% 185.3 / 12.1 116V278F (GTT→TTT) ygeVputative sigma‑54‑interacting transcriptional activator
*CP009273104,0410GT23.6% 211.7 / 28.4 110intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP009273838,6520TG23.2% 280.2 / 12.0 142F289V (TTT→GTT) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP009273838,6750CA22.8% 326.6 / 19.8 158N296K (AAC→AAArlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP0092732,178,9080TG22.7% 329.2 / 24.1 154intergenic (‑128/+95)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
*CP0092734,008,4530GT22.0% 359.8 / 28.5 182D681Y (GAT→TAT) metE5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
*CP0092732,170,7130CA21.7% 192.9 / 10.4 174intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP009273620,0540AT21.6% 219.0 / 12.5 92intergenic (‑88/‑287)fepB/entCiron‑enterobactin transporter subunit/isochorismate synthase 1
*CP0092734,586,6380GC21.4% 292.4 / 10.3 116E278Q (GAA→CAA) yjjNL‑galactonate oxidoreductase
*CP009273104,0330TG20.9% 253.8 / 15.0 105intergenic (+72/‑159)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP009273838,6590CG20.6% 312.1 / 16.2 142A291G (GCC→GGC) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 1203261149 (0.850)25 (0.160) 20/238 3.8 18.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 89 (0.570)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1203246 =148 (0.840)29 (0.190) 18/238 4.3 20.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 92 (0.590)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 360286NA (NA)4 (0.020)
+AATTC
4/260 10.0 2.2% intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 3416618 = 189 (1.070)intergenic (+65/+164) yhdZ/rrfF putative amino‑acid transporter subunit/5S ribosomal RNA of rrnD operon
* ? CP009273 = 1205074140 (0.790)3 (0.020) 3/240 10.2 2.1% pseudogene (2/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
?CP009273 2465376 = 157 (1.000)pseudogene (226/579 nt) yfdL pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
* ? CP009273 2311642 =125 (0.710)3 (0.020) 3/256 10.6 2.0% coding (1714/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
?CP009273 3953372 = 182 (1.090)pseudogene (243/243 nt) yifN PemK toxin family pseudogene
* ? CP009273 = 787656198 (1.120)3 (0.020) 3/266 10.8 1.6% intergenic (‑145/+116) galE/modF UDP‑galactose‑4‑epimerase/fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
?CP009273 = 788094 167 (0.960)coding (1151/1473 nt) modF fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
* ? CP009273 = 420028215 (1.220)3 (0.020) 3/266 10.8 1.5% coding (347/582 nt) acpH acyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase
?CP009273 = 2794317 180 (1.040)coding (236/246 nt) nrdH hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
* ? CP009273 = 849596230 (1.310)3 (0.020) 3/268 10.9 1.3% coding (494/1119 nt) ybiR putative transporter
?CP009273 3614126 = NA (NA)coding (1575/4236 nt) rhsB Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
* ? CP009273 485030 =162 (0.920)3 (0.020) 3/268 10.9 1.9% coding (3039/3363 nt) mscK mechanosensitive channel protein, intermediate conductance, K+ regulated
?CP009273 684667 = 149 (0.850)intergenic (‑198/+132) insH1/lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase