breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,177,5860TG67.9% 269.0 / 220.1 224L770R (CTC→CGC) rpoCRNA polymerase, beta prime subunit
*CP0092732,170,7340GT41.5% 47.6 / 78.7 142intergenic (‑51/+257)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP0092734,178,5000CT37.3% 220.6 / 246.8 209R1075C (CGT→TGT) rpoCRNA polymerase, beta prime subunit
*CP0092733,313,7650AC32.6% 265.3 / 151.4 175L403W (TTG→TGG) yhbXputative EptAB family phosphoethanolamine transferase, inner membrane protein
*CP009273418,9950TG29.9% 190.1 / 24.5 117L341R (CTG→CGG) malZmaltodextrin glucosidase
*CP0092732,178,9080TG27.8% 207.5 / 16.6 108intergenic (‑128/+95)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
*CP0092732,170,7240GT25.8% 150.5 / 17.1 136intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP0092732,998,3050GT25.6% 182.4 / 23.9 121Q281K (CAA→AAA) ygeVputative sigma‑54‑interacting transcriptional activator
*CP0092732,998,3330TA24.1% 251.5 / 28.0 117Q271H (CAA→CATygeVputative sigma‑54‑interacting transcriptional activator
*CP009273104,0420AT24.1% 211.1 / 16.4 86intergenic (+81/‑150)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP009273838,6750CA24.0% 361.6 / 22.9 172N296K (AAC→AAArlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP009273104,0330TG23.3% 177.3 / 10.6 86intergenic (+72/‑159)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
*CP0092732,170,7460CA23.1% 257.7 / 23.7 152intergenic (‑63/+245)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
*CP0092733,997,3950GC23.1% 261.7 / 28.8 110F48L (TTC→TTGrarDputative chloramphenical resistance permease
*CP009273277,6800CA22.7% 248.4 / 19.0 154M5I (ATG→ATTyagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
*CP0092732,810,9000GA22.4% 512.1 / 145.0 230intergenic (‑38/+243)yqaB/argQfructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
*CP009273838,6520TG21.7% 337.9 / 16.0 161F289V (TTT→GTT) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP0092734,402,5980CG21.6% 255.2 / 16.0 106S132C (TCT→TGT) yjfMDUF1190 family protein
*CP009273838,6720GT20.9% 358.6 / 19.5 173M295I (ATG→ATTrlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
*CP009273838,6580GT20.8% 316.7 / 20.4 159A291S (GCC→TCC) rlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Marginal new junction evidence (lowest skew 10 of 11 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 3399761 =245 (1.470)8 (0.050) 6/244 7.8 3.4% coding (486/1350 nt) accC acetyl‑CoA carboxylase, biotin carboxylase subunit
?CP009273 = 3399757 235 (1.550)coding (482/1350 nt) accC acetyl‑CoA carboxylase, biotin carboxylase subunit
* ? CP009273 = 4532507102 (0.610)4 (0.030) 3/250 9.7 3.2% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 146 (0.940)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 2886037 =214 (1.280)3 (0.020) 3/250 9.7 1.4% coding (22/1272 nt) ygcN putative oxidoreductase
?CP009273 = 2886033 211 (1.360)coding (18/1272 nt) ygcN putative oxidoreductase
* ? CP009273 = 3584869246 (1.470)3 (0.020) 3/264 10.0 1.2% coding (817/1317 nt) ugpB glycerol‑3‑phosphate transporter subunit
?CP009273 = 4229591 253 (1.540)coding (2030/2097 nt) yjbH DUF940 family extracellular polysaccharide protein
* ? CP009273 = 2282590NA (NA)3 (0.020) 3/264 10.0 2.1% noncoding (1003/1195 nt) IS5 repeat region
?CP009273 = 2616904 142 (0.850)coding (502/2067 nt) ppk polyphosphate kinase, component of RNA degradosome
* ? CP009273 = 2002623202 (1.210)3 (0.020) 3/262 10.0 1.6% coding (866/1206 nt) yedE UPF0394 family sulfur transport domain‑containing inner membrane protein
?CP009273 = 3372903 164 (1.010)coding (454/1128 nt) yhcM putative AFG1‑like family P‑loop ATPase
* ? CP009273 = 3923261199 (1.190)5 (0.030) 3/266 10.1 2.4% coding (1193/1497 nt) ravA hexameric AAA+ MoxR family ATPase, putative molecular chaperone
?CP009273 3923269 = 201 (1.210)coding (1185/1497 nt) ravA hexameric AAA+ MoxR family ATPase, putative molecular chaperone
* ? CP009273 = 2758368159 (0.950)3 (0.020) 3/268 10.1 1.7% coding (145/1617 nt) yfjL CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
?CP009273 3055585 = 185 (1.110)coding (1377/2145 nt) scpA methylmalonyl‑CoA mutase
* ? CP009273 = 859527212 (1.270)3 (0.020) 3/266 10.1 1.4% coding (430/663 nt) fsaA fructose‑6‑phosphate aldolase 1
?CP009273 = 3718282 207 (1.250)coding (397/912 nt) glyQ glycine tRNA synthetase, alpha subunit
* ? CP009273 221210 =130 (0.780)5 (0.030) 3/268 10.1 2.8% noncoding (953/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?CP009273 267669 = 221 (1.330)pseudogene (195/426 nt) insN pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related