breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 15,320:2 +C 5.1% intergenic (+22/‑125) dnaJ → / → insL1 chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
RA 25,769 G→T 5.2% intergenic (+68/‑57) lspA → / → fkpB prolipoprotein signal peptidase (signal peptidase II)/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 33,308 C→T 5.4% A831V (GCG→GTG)  carB → carbamoyl‑phosphate synthase large subunit
RA 36,156 C→A 8.3% E3* (GAA→TAA)  caiD ← carnitinyl‑CoA dehydratase
RA 62,253 C→A 6.0% D338Y (GAT→TAT)  rapA ← RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 62,703 C→A 5.1% G188W (GGG→TGG)  rapA ← RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 65,366 G→T 5.3% R139S (CGT→AGT)  polB ← DNA polymerase II
RA 110,380 G→A 6.8% P150P (CCG→CCA guaC → GMP reductase
RA 134,950 C→T 7.0% P461S (CCG→TCG)  cueO → multicopper oxidase (laccase)
RA 204,992 G→T 5.3% R1127L (CGG→CTG)  dnaE → DNA polymerase III alpha subunit
RA 209,669 G→T 8.3% L284L (CTG→CTT tilS → tRNA(Ile)‑lysidine synthetase
RA 221,425 T→C 5.5% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 5.8% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 259,285 C→T 7.8% Q32Q (CAG→CAA ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
RA 259,297 A→G 5.7% R28R (CGT→CGC ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
RA 259,308 A→G 5.4% L25L (TTA→CTA)  ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
RA 259,327 G→T 5.5% P18P (CCC→CCA ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
RA 259,330 T→C 5.5% S17S (TCA→TCG ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
RA 263,231 C→A 5.4% R162R (CGG→CGT yafZ ← CP4‑6 prophage; conserved protein
RA 263,234 G→C 5.5% D161E (GAC→GAG yafZ ← CP4‑6 prophage; conserved protein
RA 286,409 T→A 7.5% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 304,162 C→A 6.4% A294A (GCG→GCT ecpC ← ECP production outer membrane protein
RA 347,277 G→T 5.9% G118C (GGC→TGC)  prpD → 2‑methylcitrate dehydratase
RA 370,556:1 +C 5.9% intergenic (+219/‑359) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 371,448 G→A 7.1% P178P (CCG→CCA mhpT → 3‑hydroxyphenylpropionic transporter
RA 415,030 C→A 6.2% intergenic (+390/‑17) phoR → / → brnQ sensory histidine kinase in two‑component regulatory system with PhoB/branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
RA 418,995 T→G 16.8% L341R (CTG→CGG)  malZ → maltodextrin glucosidase
RA 480,424 G→T 5.5% Q218K (CAG→AAG)  acrA ← multidrug efflux system
RA 492,639 C→A 8.9% I3I (ATC→ATA adk → adenylate kinase
RA 523,461 G→A 6.2% pseudogene (54/708 nt) rhsH → pseudogene, Rhs familly protein
RA 523,476 G→T 6.1% pseudogene (69/708 nt) rhsH → pseudogene, Rhs familly protein
RA 523,482 A→G 6.3% pseudogene (75/708 nt) rhsH → pseudogene, Rhs familly protein
RA 527,950 C→A 7.3% A15D (GCT→GAT)  allA → ureidoglycolate lyase, releasing urea
RA 533,886 G→T 7.1% L266L (CTG→CTT ybbW → putative allantoin transporter
RA 576,234 G→A 6.8% intergenic (‑333/‑56) ylcI ← / → nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
RA 605,629 A→C 5.6% intergenic (‑94/+81) entD ← / ← fepA phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter
RA 628,454 G→T 7.3% L160L (CTC→CTA ybdH ← putative oxidoreductase
RA 630,999 G→T 5.6% P343T (CCA→ACA)  ybdN ← PAPS reductase‑like domain protein
RA 693,582 G→T 5.7% S351Y (TCT→TAT)  asnB ← asparagine synthetase B
RA 726,830 G→A 5.1% A598T (GCC→ACC) ‡ rhsC → Rhs family putative polymorphic toxin
RA 726,831 C→T 5.2% A598V (GCC→GTC) ‡ rhsC → Rhs family putative polymorphic toxin
RA 727,253 A→G 5.8% T739A (ACG→GCG)  rhsC → Rhs family putative polymorphic toxin
RA 732,499 T→C 6.5% pseudogene (219/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 742,940 C→T 6.0% A96T (GCC→ACC)  abrB ← regulator of aidB expression; inner membrane protein
RA 768,256 G→A 5.2% R448H (CGC→CAC)  cydA → cytochrome d terminal oxidase, subunit I
RA 900,601 C→A 5.4% P78Q (CCA→CAA)  amiD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein
RA 930,967 C→A 9.3% P763Q (CCG→CAG)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 1,001,734 G→A 8.9% P109P (CCG→CCA zapC → FtsZ stabilizer
RA 1,009,323 C→A 6.1% F203L (TTC→TTA pqiB → paraquat‑inducible, SoxRS‑regulated MCE domain protein
RA 1,023,572 C→T 6.4% G57G (GGC→GGT yccU → putative CoA‑binding protein
RA 1,027,272 C→A 5.5% intergenic (‑104/‑323) serT ← / → hyaA tRNA‑Ser/hydrogenase 1, small subunit
RA 1,045,283 T→G 5.9% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,050,522 C→A 5.1% L371L (CTG→CTT torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,130,531 G→A 5.5% R116H (CGT→CAT)  flgG → flagellar component of cell‑distal portion of basal‑body rod
RA 1,130,826 G→A 7.3% L214L (CTG→CTA flgG → flagellar component of cell‑distal portion of basal‑body rod
RA 1,159,779 C→A 6.4% R77S (CGC→AGC)  nagZ → beta N‑acetyl‑glucosaminidase
RA 1,203,106 G→T 6.0% V50V (GTG→GTT stfP → e14 prophage; uncharacterized protein
RA 1,203,115 A→C 5.5% A53A (GCA→GCC stfP → e14 prophage; uncharacterized protein
RA 1,225,749 G→T 9.4% V34V (GTC→GTA hlyE ← hemolysin E
RA 1,231,187 G→T 5.8% P504Q (CCG→CAG)  ycgB ← SpoVR family stationary phase protein
RA 1,265,353 T→G 8.8% noncoding (40/66 nt) rdlB → sRNA antisense regulator affects LdrB translation; proposed addiction module in LDR‑B repeat, with toxic peptide LdrB
RA 1,282,249 A→G 9.9% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 8.4% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 8.1% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 10.1% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,325,829 G→T 8.7% V175V (GTG→GTT topA → DNA topoisomerase I, omega subunit
RA 1,333,429 G→T 5.9% intergenic (‑12/‑158) ribA ← / → pgpB GTP cyclohydrolase II/phosphatidylglycerophosphatase B
RA 1,370,777 C→A 6.5% R163S (CGC→AGC)  ycjR → putative TIM alpha/beta barrel enzyme
RA 1,379,249 C→A 5.4% L292L (CTC→CTA ycjX → DUF463 family protein, putative P‑loop NTPase
RA 1,397,783 C→T 6.3% W347* (TGG→TAG)  abgA ← p‑aminobenzoyl‑glutamate hydrolase, A subunit
RA 1,422,040 A→G 6.0% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 7.3% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 7.6% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 7.9% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,681 A→G 7.1% pseudogene (303/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,444,044 C→T 5.1% A521A (GCG→GCA tynA ← tyramine oxidase, copper‑requiring
RA 1,460,458 T→C 5.3% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,467 G→A 5.4% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,752 C→T 6.3% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,819 G→A 11.2% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,481,794 C→A 5.4% I101I (ATC→ATA ydcF → DUF218 superfamily protein, SAM‑binding
RA 1,488,355 C→A 5.3% F543L (TTC→TTA trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,522,209 C→T 7.4% pseudogene (51/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,525,824 C→A 5.3% pseudogene (243/315 nt) yncI → pseudogene
RA 1,527,189 A→G 9.2% T373A (ACG→GCG)  ydcC → H repeat‑associated putative transposase
RA 1,539,135 C→A 7.1% pseudogene (837/951 nt) yddK ← pseudogene, leucine‑rich protein; putative glycoportein
RA 1,624,086 C→A 9.5% R362L (CGA→CTA)  ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,634,577 C→A 6.9% E16* (GAG→TAG)  ydfR ← Qin prophage; uncharacterized protein
RA 1,657,607 G→A 5.3% G121R (GGG→AGG)  ynfG → oxidoreductase, Fe‑S subunit
RA 1,665,715 C→A 5.6% P28H (CCT→CAT)  asr → acid shock‑inducible periplasmic protein
RA 1,668,941 T→C 6.7% F258L (TTC→CTC)  tqsA → pheromone AI‑2 transporter
RA 1,672,380 C→A 6.8% intergenic (‑220/‑304) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
RA 1,687,647 C→A 5.3% G292C (GGT→TGT)  uidB ← glucuronide transporter
RA 1,719,078 G→T 5.2% A271E (GCA→GAA)  ydhF ← putative oxidoreductase
RA 1,728,786 G→T 8.5% R32M (AGG→ATG)  mepH → murein DD‑endopeptidase, space‑maker hydrolase
RA 1,730,099 C→A 6.2% T155T (ACC→ACA sodB → superoxide dismutase, Fe
RA 1,746,843 C→A 8.7% A415A (GCG→GCT ydhV ← putative oxidoreductase subunit
RA 1,829,789 T→C 5.2% L6L (CTT→CTC ynjA → carboxymuconolactone decarboxylase family protein
RA 1,830,471 C→A 7.4% R48S (CGC→AGC)  ynjB → putative ABC superfamily transporter periplasmic‑binding protein
RA 1,833,486 C→A 5.1% F161L (TTC→TTA ynjD → putative ABC superfamily transporter ATP‑binding subunit
RA 1,870,372 C→T 5.2% A148V (GCA→GTA)  yeaN → putative transporter
RA 1,878,275 C→A 11.8% V277V (GTC→GTA yeaV → putative transporter
RA 1,882,553 G→A 8.0% A484V (GCG→GTG)  fadD ← acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
RA 1,895,294 G→T 5.5% P183P (CCC→CCA yoaE ← putative membrane protein/conserved protein
RA 1,900,961 C→A 8.9% K119N (AAG→AAT rlmA ← 23S rRNA m(1)G745 methyltransferase, SAM‑dependent
RA 1,961,275 T→C 5.8% K7R (AAA→AGA)  cheZ ← chemotaxis regulator, protein phosphatase for CheY
RA 1,964,631 C→A 5.8% S204I (AGC→ATC)  tap ← methyl‑accepting protein IV
RA 1,984,025 C→A 8.2% L288L (CTC→CTA tyrP → tyrosine transporter
RA 2,085,551 C→A 5.3% P325Q (CCG→CAG)  hisD → bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
RA 2,087,495 C→T 5.1% Q183* (CAG→TAG)  hisB → fused histidinol‑phosphatase/imidazoleglycerol‑phosphate dehydratase
RA 2,150,962 C→A 5.3% A740D (GCC→GAC)  mdtB → multidrug efflux system, subunit B
RA 2,196,141 C→A 5.7% T795K (ACG→AAG)  yehI → DUF4132 domain‑containing protein
RA 2,196,145 C→G 5.5% F796L (TTC→TTG yehI → DUF4132 domain‑containing protein
RA 2,196,148 C→A 5.6% C797* (TGC→TGA yehI → DUF4132 domain‑containing protein
RA 2,196,151 Δ1 bp 5.6% coding (2394/3633 nt) yehI → DUF4132 domain‑containing protein
RA 2,249,462 C→A 5.4% P210Q (CCG→CAG)  yeiL → putative transcriptional regulator
RA 2,249,621 T→C 8.2% A398A (GCA→GCG psuT ← putative nucleoside transporter
RA 2,266,064 C→T 5.2% G74G (GGC→GGT yejA → microcin C transporter YejABEF, periplasmic binding protein; ABC family
RA 2,275,962 C→A 5.7% intergenic (+91/‑34) yejH → / → rplY putative ATP‑dependent DNA or RNA helicase/50S ribosomal subunit protein L25
RA 2,304,384 C→T 6.1% S210S (TCG→TCA apbE ← putative thiamine‑synthetic flavin transferase lipoprotein
RA 2,320,948 C→A 5.7% T225T (ACG→ACT yfaP ← DUF2135 family protein, putative host defense protein
RA 2,352,147 G→T 6.6% Q60K (CAG→AAG)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
RA 2,383,825 G→T 5.3% P387Q (CCG→CAG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N
RA 2,391,302 C→A 5.8% P781P (CCG→CCT nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,465,390 T→G 7.5% pseudogene (212/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,465,398 C→A 8.5% pseudogene (204/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,465,404 G→A 9.1% pseudogene (198/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,465,408 A→G 6.7% pseudogene (194/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,497,475 C→A 6.8% E164* (GAG→TAG)  fryA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
RA 2,503,891 G→T 6.7% L261L (CTG→CTT yfeO → putative ion channel protein
RA 2,514,609 Δ1 bp 6.3% intergenic (+2/‑3) valY → / → lysV tRNA‑Val/tRNA‑Lys
RA 2,514,610 Δ1 bp 6.3% intergenic (+3/‑2) valY → / → lysV tRNA‑Val/tRNA‑Lys
RA 2,541,735 C→T 7.6% A92V (GCC→GTC)  yfeW → penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity
RA 2,549,778 C→A 5.3% M293I (ATG→ATT eutC ← ethanolamine ammonia‑lyase, small subunit (light chain)
RA 2,587,120 C→A 5.6% D4Y (GAC→TAC)  ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,641,359 C→A 8.1% A1430S (GCG→TCG)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,682,911 A→T 5.7% intergenic (‑46/+119) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,707,680 G→A 5.7% intergenic (+91/+118) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,707,703 C→T 6.7% intergenic (+114/+95) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,715,079 G→T 8.2% E590* (GAG→TAG)  pka → protein lysine acetyltransferase
RA 2,719,426 T→C 94.9% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,719,536 G→T 12.9% noncoding (12/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,742,425 C→A 11.4% T98N (ACT→AAT)  yfjD → UPF0053 family inner membrane protein
RA 2,745,508 G→T 8.4% E119* (GAA→TAA)  recN → recombination and repair protein
RA 2,748,152 C→A 7.6% intergenic (‑29/‑103) ratA ← / → smpB toxic UPF0083 family protein inhibitor of 70S ribosome formation/trans‑translation protein
RA 2,861,107 C→A 9.5% E336* (GAA→TAA)  nlpD ← activator of AmiC murein hydrolase activity, lipoprotein
RA 2,876,051 G→T 11.4% R483S (CGC→AGC)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 3,020,257 G→T 5.2% G378V (GGC→GTC)  guaD → guanine deaminase
RA 3,027,995 G→T 5.5% S180Y (TCC→TAC)  lysS ← lysine tRNA synthetase, constitutive
RA 3,043,956 C→A 6.1% W24L (TGG→TTG)  gcvT ← aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
RA 3,064,718 T→G 10.4% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,080,755 T→G 7.1% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase
RA 3,096,890 C→A 5.6% P173P (CCC→CCA mutY → adenine DNA glycosylase
RA 3,161,125 G→T 5.7% intergenic (‑48/+85) ygiS ← / ← mqsA putative inner membrane ABC superfamily transporter permease/antitoxin for MqsR toxin; transcriptional repressor
RA 3,181,434 C→A 5.7% pseudogene (655/2445 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
RA 3,199,759:1 +C 6.1% intergenic (‑144/‑63) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,199,762 T→A 6.1% intergenic (‑147/‑60) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,199,763 T→G 6.7% intergenic (‑148/‑59) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,199,764 T→G 6.1% intergenic (‑149/‑58) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,199,769:1 +T 6.2% intergenic (‑154/‑53) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,199,769:2 +G 6.2% intergenic (‑154/‑53) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,199,773 A→C 5.9% intergenic (‑158/‑49) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,207,186 C→T 5.7% L261L (CTG→TTG)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,224,756 C→A 6.7% intergenic (+158/‑268) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,263,315 T→C 7.4% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,328,329 G→T 6.5% G21V (GGA→GTA)  sfsB → malPQ operon transcriptional activator
RA 3,345,000 C→A 5.5% G462V (GGC→GTC)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,387,173 C→A 5.1% A815A (GCG→GCT yhdP ← DUF3971‑AsmA2 domains protein
RA 3,416,899 A→G 8.1% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,465,459 C→T 6.0% R472H (CGT→CAT)  fusA ← protein chain elongation factor EF‑G, GTP‑binding
RA 3,466,087 G→T 9.0% L263M (CTG→ATG)  fusA ← protein chain elongation factor EF‑G, GTP‑binding
RA 3,539,549 C→A 5.5% S189S (TCC→TCA nfuA → Fe/S biogenesis protein; putative scaffold/chaperone for damaged Fe/S proteins
RA 3,548,657 C→A 5.5% Y738* (TAC→TAA malT → mal regulon transcriptional activator
RA 3,551,754 G→T 7.2% R43L (CGA→CTA)  rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,608,154 A→T 6.8% K376N (AAA→AAT nikA → nickel‑binding, heme‑binding periplasmic protein
RA 3,616,199 A→G 5.3% R1216R (CGA→CGG rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 5.6% G141G (GGA→GGG yhhI → putative transposase
RA 3,670,805 G→T 8.8% T302T (ACC→ACA yhjG ← putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,760,562 G→T 5.5% intergenic (+23/‑19) yibA → / → rhsJ HEAT‑domain lethality reduction protein; putative immunity protein for putative polymorphic toxin RhsA/Rhs‑family protein
RA 3,760,566 A→G 5.3% intergenic (+27/‑15) yibA → / → rhsJ HEAT‑domain lethality reduction protein; putative immunity protein for putative polymorphic toxin RhsA/Rhs‑family protein
RA 3,760,943 G→A 5.3% pseudogene (363/945 nt) rhsJ → Rhs‑family protein
RA 3,838,099 C→A 7.2% S259Y (TCC→TAC)  ade → cryptic adenine deaminase
RA 3,869,212 G→T 7.7% G139C (GGT→TGT)  yidX → putative lipoprotein
RA 3,877,923 A→G 100% Q24R (CAG→CGG)  rnpA → protein C5 component of RNase P
RA 3,901,866 C→G 6.5% intergenic (‑140/+43) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 3,901,867 T→G 7.1% intergenic (‑141/+42) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 3,918,853 G→T 5.8% C47* (TGC→TGA mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,935,371 G→T 6.3% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,936,724 T→A 11.3% intergenic (+15/‑71) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 3,950,334 C→T 6.0% E283K (GAG→AAG)  ilvY ← transcriptional activator of ilvC; autorepressor
RA 3,950,336 T→G 6.0% H282P (CAT→CCT) ‡ ilvY ← transcriptional activator of ilvC; autorepressor
RA 3,950,337 G→T 6.0% H282N (CAT→AAT) ‡ ilvY ← transcriptional activator of ilvC; autorepressor
RA 3,952,869 T→G 18.3% intergenic (+64/+23) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,016,182 G→T 5.1% V29V (GTG→GTT tatC → TatABCE protein translocation system subunit
RA 4,019,993 Δ1 bp 100% coding (107/702 nt) fre → NAD(P)H‑flavin reductase
RA 4,161,576 A→C 10.2% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,165,449 C→T 17.0% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 17.2% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,177,142 A→T 6.8% D622V (GAC→GTC)  rpoC → RNA polymerase, beta prime subunit
RA 4,177,586 T→G 81.8% L770R (CTC→CGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,193,073 C→A 6.6% A407D (GCC→GAC)  zraS → sensory histidine kinase in two‑component regulatory system with ZraR
RA 4,199,631 T→A 9.4% intergenic (+15/‑71) rrsE → / → gltV 16S ribosomal RNA of rrnE operon/tRNA‑Glu
RA 4,202,979 A→C 10.8% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,269,099 C→A 5.5% A231A (GCC→GCA yjcD → inner membrane putative guanine permease
RA 4,283,150 C→A 5.8% V20V (GTC→GTA nrfF → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF
RA 4,315,765 A→G 7.6% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,336,205 G→A 5.1% G313G (GGC→GGT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,238 A→G 6.5% R302R (CGT→CGC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,241 G→A 5.7% I301I (ATC→ATT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,361,922 G→T 6.1% D361Y (GAC→TAC)  groL → Cpn60 chaperonin GroEL, large subunit of GroESL
RA 4,372,570 G→T 5.4% E37D (GAG→GAT epmA → Elongation Factor P Lys34 lysyltransferase
RA 4,374,660 G→T 6.5% P335P (CCG→CCT yjeM → putative transporter
RA 4,381,668 G→T 5.2% R83L (CGG→CTG)  orn → oligoribonuclease
RA 4,391,954 C→A 7.2% R34S (CGC→AGC)  hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 4,399,868 T→C 10.4% intergenic (+45/‑82) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
RA 4,400,031 G→A 7.7% A28T (GCA→ACA)  yjfI → DUF2170 family protein
RA 4,449,764 G→T 7.3% L140M (CTG→ATG)  nrdG ← anaerobic ribonucleotide reductase activating protein
RA 4,498,906 T→C 5.0% pseudogene (81/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,909 G→T 5.3% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,660 T→G 5.4% pseudogene (123/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,885 T→A 7.3% pseudogene (348/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,516,519 C→A 9.3% D63Y (GAC→TAC)  sgcR ← putative DNA‑binding transcriptional regulator
RA 4,604,253 T→G 7.7% intergenic (+176/+244) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,320 T→G 7.1% intergenic (+243/+177) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,322 A→C 6.0% intergenic (+245/+175) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,617,948 G→A 8.9% G273S (GGC→AGC)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
RA 4,625,681 C→A 6.8% S115* (TCG→TAG)  creA → putative periplasmic protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 463833 464308 476 24 [14] [20] 21 ybaO ybaO
* * ÷ CP009273 3448726 3448865 140 22 [19] [9] 27 [gspA] [gspA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =87 (0.950)76 (0.830) 34/250 NT 46.6% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =37 (0.410)43 (0.540) 15/218 NT 60.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 23 (0.290)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326136 (0.390)32 (0.400) 12/218 NT 54.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 23 (0.290)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1972769 =6 (0.070)109 (1.200) 40/248 NT 94.8% intergenic (‑315/‑465) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 19727774 (0.040)78 (0.850) 36/250 NT 95.1% intergenic (‑323/‑457) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E