breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 4,173,025 | 0 | A | T | 48.8% | 9.6 / 230.2 | 124 | Q618L (CAG→CTG) | rpoB | RNA polymerase, beta subunit |
* | CP009273 | 2,170,734 | 0 | G | T | 42.2% | 37.5 / 30.1 | 71 | intergenic (‑51/+257) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I |
* | CP009273 | 1,630,704 | 0 | C | G | 28.9% | 79.0 / 10.3 | 49 | intergenic (‑80/‑309) | nohQ/ynfO | pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage |
* | CP009273 | 104,042 | 0 | A | T | 28.3% | 118.3 / 16.2 | 55 | intergenic (+81/‑150) | lpxC/secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
* | CP009273 | 2,998,333 | 0 | T | A | 27.0% | 156.1 / 18.3 | 74 | Q271H (CAA→CAT) | ygeV | putative sigma‑54‑interacting transcriptional activator |
* | CP009273 | 104,041 | 0 | G | T | 26.8% | 101.4 / 14.6 | 56 | intergenic (+80/‑151) | lpxC/secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
* | CP009273 | 2,178,908 | 0 | T | G | 24.3% | 132.6 / 11.9 | 70 | intergenic (‑128/+95) | thiM/rcnR | hydoxyethylthiazole kinase/transcriptional repressor of rcnA |
* | CP009273 | 3,997,395 | 0 | G | C | 23.3% | 233.4 / 11.8 | 96 | F48L (TTC→TTG) | rarD | putative chloramphenical resistance permease |
* | CP009273 | 838,672 | 0 | G | T | 23.3% | 140.5 / 14.8 | 73 | M295I (ATG→ATT) | rlmF | 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent |
* | CP009273 | 104,048 | 0 | A | T | 22.8% | 139.9 / 10.4 | 57 | intergenic (+87/‑144) | lpxC/secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
* | CP009273 | 277,680 | 0 | C | A | 22.7% | 184.2 / 11.1 | 97 | M5I (ATG→ATT) | yagA | CP4‑6 prophage; putative DNA‑binding transcriptional regulator |
* | CP009273 | 3,915,253 | 0 | A | C | 20.3% | 265.6 / 60.8 | 118 | G53G (GGT→GGG) | atpB | F0 sector of membrane‑bound ATP synthase, subunit a |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | = 807530 | 101 (1.070) | 3 (0.040) +CTGTCTCTTATAC |
3/242 | 6.9 | 3.6% | coding (553/756 nt) | bioC | malonyl‑ACP O‑methyltransferase, SAM‑dependent |
? | CP009273 | = 1750012 | 79 (0.840) | coding (58/1413 nt) | pykF | pyruvate kinase I | |||||
* | ? | CP009273 | 3373977 = | 99 (1.050) | 3 (0.030) | 3/266 | 7.3 | 3.7% | intergenic (+29/‑125) | yhcB/degQ | DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic |
? | CP009273 | = 3375215 | 59 (0.630) | coding (1114/1368 nt) | degQ | serine endoprotease, periplasmic | |||||
* | ? | CP009273 | 472726 = | 96 (1.020) | 3 (0.030) | 3/266 | 7.3 | 3.2% | coding (1348/1551 nt) | ylaB | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
? | CP009273 | 2585859 = | 86 (0.920) | coding (894/1128 nt) | dapE | N‑succinyl‑diaminopimelate deacylase |