breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 20,724 G→A 5.1% intergenic (‑216/+91) insA ← / ← rpsT IS1 repressor TnpA/30S ribosomal subunit protein S20
RA 27,126 C→A 6.1% Q284K (CAG→AAG)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase, 4Fe‑4S protein
RA 218,831 C→A 5.5% G101V (GGC→GTC)  metN ← DL‑methionine transporter subunit
RA 235,769 G→T 5.2% pseudogene (177/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 249,908 A→G 6.8% intergenic (+260/‑58) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 249,918 T→A 8.0% intergenic (+270/‑48) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 249,924 T→C 7.9% intergenic (+276/‑42) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 259,937 T→G 5.9% R8R (AGG→CGG)  ykfH ← uncharacterized protein
RA 274,938 T→A 5.8% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 277,658 T→G 5.3% M13L (ATG→CTG)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator
RA 345,384 G→C 5.6% intergenic (+101/‑339) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 345,387 G→A 5.1% intergenic (+104/‑336) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 345,428 T→C 5.1% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 353,317 C→T 6.0% intergenic (+152/+185) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 372,807 Δ1 bp 7.4% intergenic (+40/+184) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,808 G→A 7.5% intergenic (+41/+183) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 513,343 G→A 6.8% T131T (ACC→ACT ybbN ← DnaK co‑chaperone, thioredoxin‑like protein
RA 656,982 A→G 9.1% intergenic (‑148/+111) ybeF ← / ← lipB LysR family putative transcriptional regulator/octanoyltransferase; octanoyl‑[ACP]:protein N‑octanoyltransferase
RA 657,031 T→C 9.5% intergenic (‑197/+62) ybeF ← / ← lipB LysR family putative transcriptional regulator/octanoyltransferase; octanoyl‑[ACP]:protein N‑octanoyltransferase
RA 692,593 Δ1 bp 100% intergenic (‑4/+376) metT ← / ← asnB tRNA‑Met/asparagine synthetase B
RA 694,919 G→T 8.8% intergenic (‑286/+111) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,921 A→G 5.6% intergenic (‑288/+109) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,922 T→C 9.1% intergenic (‑289/+108) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,942 G→C 5.5% intergenic (‑309/+88) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,943 G→C 5.5% intergenic (‑310/+87) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,945 G→T 6.7% intergenic (‑312/+85) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,947 T→C 6.6% intergenic (‑314/+83) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 694,948 G→T 6.7% intergenic (‑315/+82) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 726,100 A→G 5.6% R354R (CGA→CGG rhsC → Rhs family putative polymorphic toxin
RA 726,110 T→C 6.7% C358R (TGT→CGT)  rhsC → Rhs family putative polymorphic toxin
RA 727,369 A→G 5.1% A777A (GCA→GCG rhsC → Rhs family putative polymorphic toxin
RA 727,375 A→G 5.1% G779G (GGA→GGG rhsC → Rhs family putative polymorphic toxin
RA 811,097 G→A 8.2% intergenic (+94/+98) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,098 C→A 6.5% intergenic (+95/+97) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,100:1 +A 5.8% intergenic (+97/+95) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 811,146 C→A 6.6% intergenic (+143/+49) uvrB → / ← ybhK excinulease of nucleotide excision repair, DNA damage recognition component/putative NAD(P)‑binding transferase
RA 859,422 A→C 7.0% T109P (ACC→CCC)  fsaA → fructose‑6‑phosphate aldolase 1
RA 893,516:1 +C 8.6% coding (72/489 nt) ybjO → DUF2593 family inner membrane protein
RA 893,516:2 +A 8.6% coding (72/489 nt) ybjO → DUF2593 family inner membrane protein
RA 893,517:1 +A 8.6% coding (73/489 nt) ybjO → DUF2593 family inner membrane protein
RA 893,520 C→G 7.8% Q26E (CAG→GAG) ‡ ybjO → DUF2593 family inner membrane protein
RA 893,521 A→C 8.3% Q26P (CAG→CCG) ‡ ybjO → DUF2593 family inner membrane protein
RA 893,522:1 +C 8.3% coding (78/489 nt) ybjO → DUF2593 family inner membrane protein
RA 893,522:2 +T 8.3% coding (78/489 nt) ybjO → DUF2593 family inner membrane protein
RA 910,671 T→G 11.5% intergenic (‑358/+137) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 910,682 T→G 11.8% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 920,106 C→T 8.0% Q463* (CAG→TAG)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 931,037 G→A 6.4% A786A (GCG→GCA ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 931,040 A→G 6.5% P787P (CCA→CCG ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 946,743 G→T 6.3% F756L (TTC→TTA pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 981,924 C→A 6.9% G172V (GGC→GTC)  ompF ← outer membrane porin 1a (Ia;b;F)
RA 988,636 G→T 5.6% G853G (GGG→GGT pepN → aminopeptidase N
RA 1,020,092 G→T 5.2% E56* (GAG→TAG)  helD → DNA helicase IV
RA 1,042,210 A→C 6.7% S398A (TCA→GCA)  gfcD ← putative O‑antigen capsule production periplasmic protein
RA 1,062,549 C→T 6.2% intergenic (‑2/+19) yccJ ← / ← wrbA uncharacterized protein/NAD(P)H:quinone oxidoreductase
RA 1,140,403 C→T 5.9% T3I (ACA→ATA)  rluC → 23S rRNA pseudouridine(955,2504,2580) synthase
RA 1,218,978 C→T 6.5% intergenic (+73/‑42) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,250,869 G→T 8.8% N180K (AAC→AAA ycgV ← putative adhesin
RA 1,254,694 G→T 5.5% R522R (CGC→CGA dauA ← C4‑dicarboxylic acid transporter
RA 1,264,818 G→T 12.2% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,264,930 A→T 8.9% intergenic (+85/+229) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,264,932 T→G 6.9% intergenic (+87/+227) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,266,001 G→T 7.5% intergenic (+85/+204) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
RA 1,276,748 C→A 5.8% L477M (CTG→ATG)  narG → nitrate reductase 1, alpha subunit
RA 1,282,249 A→G 10.2% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 5.1% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,283,577 C→A 5.7% K168N (AAG→AAT purU ← formyltetrahydrofolate hydrolase
RA 1,301,313 G→A 6.7% P32L (CCA→CTA)  yciU ← UPF0263 family protein
RA 1,302,193 G→T 5.2% T237N (ACC→AAC)  clsA ← cardiolipin synthase 1
RA 1,342,844 G→A 5.9% A109V (GCC→GTC)  rnb ← ribonuclease II
RA 1,344,548 C→T 6.5% G250D (GGT→GAT)  fabI ← enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
RA 1,382,083 G→A 5.3% P369P (CCG→CCA tyrR → aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
RA 1,417,757 C→G 8.6% pseudogene (300/444 nt)
P34R (CCG→CGG) 
rzpR →
rzoR →
pseudogene, Rac prophage; Bacteriophage Rz lysis protein family;Phage or Prophage Related; putative Rac prophage endopeptidase; completely contained in another CDS
Rac prophage; putative lipoprotein
RA 1,422,040 A→G 8.2% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 11.9% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 5.9% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,440,280 T→C 8.7% A48A (GCT→GCC ydbL → DUF1318 family protein
RA 1,460,458 T→C 5.7% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,467 G→A 5.8% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,515 G→C 8.5% pseudogene (867/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,527 C→T 6.7% pseudogene (879/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,689 A→G 6.6% pseudogene (1041/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,695 T→G 7.2% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,752 C→T 9.9% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,761 A→G 9.6% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,887 G→C 9.2% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,896 C→T 9.4% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,498,535 C→T 5.9% Q188* (CAG→TAG)  insQ → IS609 transposase B
RA 1,503,183 C→A 10.1% E47* (GAA→TAA)  yncJ ← uncharacterized protein
RA 1,505,066 C→A 8.0% L269L (CTC→CTA ydcR → putative DNA‑binding transcriptional regulator/putative amino transferase
RA 1,566,159 G→A 9.2% D48D (GAC→GAT gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,571,757 A→G 5.0% P40P (CCT→CCC yddB ← putative TonB‑dependent outer membrane receptor
RA 1,620,254 Δ1 bp 6.9% coding (1384/2046 nt) dcp ← dipeptidyl carboxypeptidase II
RA 1,622,276 C→T 9.3% T168M (ACG→ATG)  ydfG → NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase
RA 1,629,745 T→A 5.2% A120A (GCA→GCT stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,665,932 C→A 5.7% P100P (CCC→CCA asr → acid shock‑inducible periplasmic protein
RA 1,702,919 C→G 6.7% Q582E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,702,966 T→A 7.0% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,705,034 C→A 5.7% Q192K (CAG→AAG)  rsxG → SoxR iron‑sulfur cluster reduction factor component; putative membrane protein of electron transport complex
RA 1,710,601 G→T 5.3% S293R (AGC→AGA tyrS ← tyrosyl‑tRNA synthetase
RA 1,792,383 C→A 8.7% G273W (GGG→TGG)  pheS ← phenylalanine tRNA synthetase, alpha subunit
RA 1,797,963 G→A 5.5% pseudogene (189/1476 nt) arpB → pseudogene, ankyrin repeats
RA 1,879,439 C→A 8.1% A173E (GCG→GAG)  yeaW → putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster
RA 2,060,781 C→T 7.3% G8R (GGA→AGA)  insH1 ← IS5 transposase and trans‑activator
RA 2,086,677 G→T 5.2% E267* (GAA→TAA)  hisC → histidinol‑phosphate aminotransferase
RA 2,113,323 A→G 6.0% L106P (CTG→CCG)  wzxC ← putative colanic acid exporter
RA 2,175,459 G→T 7.9% V295V (GTG→GTT yegV → putative kinase
RA 2,298,159 T→C 5.4% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,241 T→C 5.7% intergenic (+369/+346) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,356,748 G→A 7.8% P122L (CCT→CTT)  yfaY ← inactive PncC family protein
RA 2,465,527 C→T 13.5% pseudogene (75/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,546,408 C→A 8.9% S233* (TCA→TAA)  amiA → N‑acetylmuramoyl‑l‑alanine amidase I
RA 2,707,492 A→C 13.1% K413T (AAG→ACG)  srmB → ATP‑dependent RNA helicase
RA 2,707,699 C→T 7.5% intergenic (+110/+99) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,707,703 C→T 16.3% intergenic (+114/+95) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,730,346 G→T 10.9% intergenic (+104/‑167) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,732,681 C→T 5.1% A250T (GCG→ACG)  tyrA ← fused chorismate mutase T/prephenate dehydrogenase
RA 2,739,692 C→T 8.9% intergenic (‑148/+101) rpsP ← / ← ffh 30S ribosomal subunit protein S16/Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
RA 2,740,209 C→T 6.7% A316T (GCA→ACA)  ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
RA 2,754,049 G→T 6.1% M48I (ATG→ATT yfjJ → CP4‑57 prophage; uncharacterized protein
RA 2,755,783 G→A 5.9% Q373* (CAG→TAG)  yfjK ← DEAD/H helicase‑like protein, CP4‑57 putative defective prophage
RA 2,771,529 T→C 9.0% V1519V (GTA→GTG ypjA ← adhesin‑like autotransporter
RA 2,776,199 A→G 9.1% intergenic (‑114/+225) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,776,221 T→C 9.2% intergenic (‑136/+203) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,863,882 C→A 6.4% G261V (GGC→GTC)  truD ← tRNA(Glu) pseudouridine(13) synthase
RA 3,023,253 G→A 7.3% G346G (GGC→GGT ygfT ← putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
RA 3,024,674 C→A 5.2% intergenic (‑384/‑52) ygfT ← / → uacT putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit/uric acid permease
RA 3,051,396 C→T 7.6% R125H (CGC→CAC)  serA ← D‑3‑phosphoglycerate dehydrogenase
RA 3,084,347 C→A 6.0% R214R (CGC→CGA endA → DNA‑specific endonuclease I
RA 3,237,983 G→T 8.0% Q40K (CAG→AAG)  uxaC ← uronate isomerase
RA 3,243,351 C→A 6.8% L104I (CTA→ATA)  yqjE → DUF1469 family inner membrane protein
RA 3,286,442 G→T 5.0% Q85K (CAA→AAA)  rsmI ← 16S rRNA C1402 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 3,302,464 G→T 6.0% S688R (AGC→AGA pnp ← polynucleotide phosphorylase/polyadenylase
RA 3,313,029 G→T 6.1% R345L (CGT→CTT)  argG → argininosuccinate synthetase
RA 3,323,804 G→T 5.1% intergenic (+49/+137) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 3,323,813 G→A 5.5% intergenic (+58/+128) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 3,323,815 T→G 5.5% intergenic (+60/+126) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 3,323,817 Δ1 bp 5.5% intergenic (+62/+124) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 3,384,766 A→C 9.2% F208V (TTT→GTT)  tldD ← putative peptidase
RA 3,385,662:1 +G 8.2% intergenic (‑275/+155) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein
RA 3,416,890 T→G 6.3% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 12.1% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,422,276 Δ1 bp 10.3% intergenic (‑155/‑319) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,422,282 Δ1 bp 9.1% intergenic (‑161/‑313) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,422,286 T→G 9.5% intergenic (‑165/‑309) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,498,220 T→G 12.9% intergenic (+78/+74) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,498,239 A→C 6.7% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,515,842 G→T 5.5% S90* (TCG→TAG)  hofM ← DNA catabolic putative pilus assembly protein
RA 3,524,578:1 +T 5.1% coding (1221/1725 nt) yhgE ← DUF4153 family putative inner membrane protein
RA 3,524,578:2 +G 5.1% coding (1221/1725 nt) yhgE ← DUF4153 family putative inner membrane protein
RA 3,524,578:3 +C 5.1% coding (1221/1725 nt) yhgE ← DUF4153 family putative inner membrane protein
RA 3,615,780 G→A 5.4% A1077T (GCC→ACC)  rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,630,389 G→A 5.8% R128C (CGT→TGT)  yhiN ← putative oxidoreductase
RA 3,634,142 C→T 6.0% intergenic (+237/‑80) uspA → / → dtpB universal stress global response regulator/dipeptide and tripeptide permease B
RA 3,669,589 A→G 6.1% intergenic (+121/+61) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,669,597 C→T 6.1% intergenic (+129/+53) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,758,716 A→G 5.1% R1058R (AGA→AGG rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,935,371 G→T 6.9% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,961,292 T→C 5.8% V6A (GTG→GCG)  wecA → UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
RA 3,991,416 T→G 17.8% L25R (CTT→CGT)  uvrD → DNA‑dependent ATPase I and helicase II
RA 4,014,141 C→A 5.2% R186S (CGT→AGT)  ubiB → regulator of octaprenylphenol hydroxylation, ubiquinone synthesis; regulator of 2'‑N‑acetyltransferase; putative ABC1 family protein kinase
RA 4,033,888 A→C 20.1% noncoding (12/120 nt) rrfA → 5S ribosomal RNA of rrnA operon
RA 4,054,006 G→A 6.8% G67E (GGG→GAG)  yihL → putative DNA‑binding transcriptional regulator
RA 4,098,583 G→A 6.8% intergenic (+141/‑179) pfkA → / → sbp 6‑phosphofructokinase I/sulfate transporter subunit
RA 4,165,449 C→T 19.0% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 19.0% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,167,038 A→T 5.7% V389V (GTA→GTT tufB → translation elongation factor EF‑Tu 2
RA 4,167,041 A→T 5.6% A390A (GCA→GCT tufB → translation elongation factor EF‑Tu 2
RA 4,178,500 C→T 91.3% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,202,979 A→C 40.0% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,285,988 C→T 5.2% intergenic (+266/+265) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,375,474 G→T 14.8% D89Y (GAT→TAT)  yjeN → uncharacterized protein
RA 4,454,076 G→T 5.7% R151S (CGC→AGC)  treC ← trehalose‑6‑P hydrolase
RA 4,467,949 T→A 6.5% R60R (CGA→CGT argI ← ornithine carbamoyltransferase 1
RA 4,471,348 G→A 5.7% C769C (TGC→TGT valS ← valyl‑tRNA synthetase
RA 4,498,906 T→C 6.4% pseudogene (81/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,498,909 G→T 6.8% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,604,111 T→C 5.6% intergenic (+34/+386) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,253 T→G 7.4% intergenic (+176/+244) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,311 G→A 7.9% intergenic (+234/+186) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,316 C→T 6.9% intergenic (+239/+181) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,320 T→C 12.8% intergenic (+243/+177) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,321 A→G 6.4% intergenic (+244/+176) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,341 Δ1 bp 5.4% intergenic (+264/+156) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,397 G→A 7.7% intergenic (+320/+100) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2027296 2027390 95 17 [8] [16] 18 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 2897085 2897256 172 19 [15] [12] 17 ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
* * ÷ CP009273 3432085 3432507 423 17 [10] [15] 18 [zntR]–[yhdN] [zntR],[yhdN]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 69011 =136 (1.360)11 (0.130) 5/206 NT 6.1% coding (404/699 nt) thiQ thiamine, thiamine pyrophosphate ABC transporter ATPase; ThiBPQ thiamine, thiamine pyrophosphate importer
?CP009273 = 3582121 226 (2.740)coding (420/1071 nt) ugpC glycerol‑3‑phosphate transporter subunit
* ? CP009273 360104 =96 (0.960)114 (1.140) 42/250 NT 54.3% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370378 =NA (NA)14 (0.150) 4/234 NT NA intergenic (+41/‑537) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 372785 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 = 370385129 (1.770)14 (0.210) 6/164 NT 10.8% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 370386 =NA (NA)18 (0.280) 8/164 NT 13.9% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 124 (1.710)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 = 370414129 (1.290)5 (0.070) 4/176 NT 5.8% noncoding (29/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 = 372848 72 (1.020)noncoding (58/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 370580 =100 (1.000)6 (0.090) 4/166 NT 5.9% noncoding (195/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372986 = 124 (1.870)intergenic (+219/+5) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 = 370587101 (1.390)10 (0.150) 7/166 NT 9.8% noncoding (202/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 1203246 =49 (0.490)23 (0.260) 13/218 NT 43.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 16 (0.180)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326143 (0.430)23 (0.260) 20/218 NT 46.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 16 (0.180)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 197277741 (0.410)12 (0.120) 5/250 NT 22.6% intergenic (‑323/‑457) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 1972854 =10 (0.100)63 (0.630) 35/250 NT 86.3% intergenic (‑400/‑380) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 197286113 (0.130)62 (0.620) 28/250 NT 82.7% intergenic (‑407/‑373) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 2298543NA (NA)3 (0.040) 3/188 NT NA noncoding (648/655 nt) REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences
?CP009273 = 4478328 NA (NA)noncoding (7/36 nt) REP343 (repetitive extragenic palindromic) element; contains 1 REP sequences REP343 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? CP009273 = 332381868 (0.960)8 (0.120) 3/174 NT 10.7% intergenic (+63/+123) dacB/obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
?CP009273 = 3591855 NA (NA)noncoding (99/99 nt) RIP259 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP259 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site