breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 161,056 | 0 | C | G | 46.7% | 12.9 / 17.4 | 30 | intergenic (+35/‑161) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
* | CP009273 | 2,170,734 | 0 | G | T | 33.3% | 85.0 / 46.8 | 108 | intergenic (‑51/+257) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I |
* | CP009273 | 4,402,596 | 0 | T | G | 28.8% | 132.8 / 15.7 | 73 | V131V (GTT→GTG) | yjfM | DUF1190 family protein |
* | CP009273 | 2,170,724 | 0 | G | T | 24.3% | 115.6 / 16.9 | 104 | intergenic (‑41/+267) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I |
* | CP009273 | 4,402,656 | 0 | C | A | 23.9% | 150.6 / 14.0 | 72 | N151K (AAC→AAA) | yjfM | DUF1190 family protein |
* | CP009273 | 4,375,475 | 0 | A | T | 22.4% | 210.9 / 13.8 | 86 | D89V (GAT→GTT) | yjeN | uncharacterized protein |
* | CP009273 | 104,042 | 0 | A | T | 22.2% | 176.5 / 10.5 | 64 | intergenic (+81/‑150) | lpxC/secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
* | CP009273 | 1,159,992 | 0 | C | G | 21.6% | 268.1 / 10.8 | 98 | R148G (CGG→GGG) | nagZ | beta N‑acetyl‑glucosaminidase |
* | CP009273 | 4,008,453 | 0 | G | T | 20.3% | 254.3 / 15.9 | 123 | D681Y (GAT→TAT) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 1203246 = | 129 (1.140) | 17 (0.170) | 13/236 | 3.8 | 15.5% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | 1205075 = | 72 (0.730) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | = 1203261 | 126 (1.120) | 21 (0.210) | 10/236 | 4.7 | 18.8% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | = 1205058 | 71 (0.720) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | = 4532507 | 60 (0.530) | 8 (0.080) | 5/250 | 7.1 | 11.7% | intergenic (+57/‑425) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | CP009273 | = 4532803 | 65 (0.620) | intergenic (+353/‑129) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) | |||||
* | ? | CP009273 | 3399761 = | 156 (1.380) | 3 (0.030) | 3/244 | 8.2 | 2.1% | coding (486/1350 nt) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
? | CP009273 | = 3399757 | 134 (1.310) | coding (482/1350 nt) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit | |||||
* | ? | CP009273 | 3785739 = | 126 (1.120) | 3 (0.030) | 3/260 | 8.5 | 2.5% | coding (173/1197 nt) | kbl | glycine C‑acetyltransferase |
? | CP009273 | = 4430062 | 108 (0.990) | coding (971/1344 nt) | ytfL | UPF0053 family inner membrane protein | |||||
* | ? | CP009273 | 2706903 = | 172 (1.530) | 3 (0.030) | 3/262 | 8.5 | 1.8% | coding (649/1335 nt) | srmB | ATP‑dependent RNA helicase |
? | CP009273 | = 2990686 | NA (NA) | noncoding (365/1331 nt) | IS2 | repeat region | |||||
* | ? | CP009273 | 2009947 = | 150 (1.330) | 3 (0.030) | 3/258 | 8.5 | 2.1% | coding (599/687 nt) | fliH | negative regulator of FliI ATPase activity |
? | CP009273 | = 2010284 | 132 (1.220) | coding (250/1374 nt) | fliI | flagellum‑specific ATP synthase | |||||
* | ? | CP009273 | = 1959840 | 111 (0.980) | 3 (0.030) | 3/262 | 8.5 | 2.6% | coding (609/1149 nt) | flhB | flagellin export apparatus, substrate specificity protein |
? | CP009273 | 2469895 = | 112 (1.020) | coding (107/201 nt) | torI | response regulator inhibitor for tor operon | |||||
* | ? | CP009273 | 956188 = | 187 (1.660) | 3 (0.030) | 3/262 | 8.5 | 1.8% | coding (469/765 nt) | ycaL | putative peptidase‑related chaperone |
? | CP009273 | = 2501044 | 139 (1.260) | coding (330/1248 nt) | fryC | putative enzyme IIC component of PTS | |||||
* | ? | CP009273 | 3923165 = | 99 (0.880) | 3 (0.030) | 3/266 | 8.6 | 2.3% | coding (1289/1497 nt) | ravA | hexameric AAA+ MoxR family ATPase, putative molecular chaperone |
? | CP009273 | 4406996 = | 153 (1.370) | coding (228/750 nt) | yjfP | acyl CoA esterase |