Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,159,112 G→A C208Y (TGT→TAT)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,159,1120GA87.5% 265.6 / 26.9 104C208Y (TGT→TAT) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (6/7);  new base A (40/51);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 104 total aligned reads displayed.

CGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATC  >  NC_000913/4158965‑4159258
                                                                                                                                                   |                                                                                                                                                  
cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTAttt                                                                                                                                                 >  1:304560/1‑151 (MQ=255)
 gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTAtttt                                                                                                                                                <  2:1131571/151‑1 (MQ=255)
  ttgttTGATGAGCCAGTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATAAAAAAAAAGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTg                                                                                                                                               <  2:1534898/151‑1 (MQ=255)
  ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTg                                                                                                                                               <  2:715049/151‑1 (MQ=255)
    gttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa                                                                                                                                             >  1:1711584/1‑151 (MQ=255)
     tttGCTGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAg                                                                                                                                            <  2:34805/151‑1 (MQ=255)
     ttggatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAg                                                                                                                                            <  1:148168/148‑1 (MQ=255)
         atgaGCCAATGTTGCTGGCTATATATGAAGAGCACCCGTGGAAGAAACGCGGATGCGTACCGATGGCCGAAAAAAAAAAAAAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCgg                                                                                                                                        <  2:882990/151‑1 (MQ=255)
          tgaGCCAATGTTGTTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAATACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGTTCAGGCAATGGGTTTCTATTTTGAAGCCggg                                                                                                                                       <  2:1063209/151‑1 (MQ=255)
          tgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCggg                                                                                                                                       >  1:1916795/1‑151 (MQ=255)
             gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCg                                                                                                                                    <  1:287652/151‑1 (MQ=255)
               cAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGa                                                                                                                                  <  2:604006/151‑1 (MQ=255)
                aaTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGAt                                                                                                                                 >  1:1132138/1‑151 (MQ=255)
                aaTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTCATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGAt                                                                                                                                 <  2:604997/151‑1 (MQ=255)
                 aTGTTGCTGGCTATCTATGAAGATCACTCGTGGGCGACCCGCGAATGCGTACCGATGGCCGATCTGGCAGAGGAACAACTGCTGATGCTAGAAGATGGTCACTGTTTTCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCCGATg                                                                                                                                <  2:116468/151‑1 (MQ=255)
                  tGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGa                                                                                                                               <  2:958813/151‑1 (MQ=255)
                   gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGaa                                                                                                                              <  2:1358965/151‑1 (MQ=255)
                   gTTGCTGGCTATCTATGAAGAGCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGaa                                                                                                                              <  2:1797419/151‑1 (MQ=255)
                    ttGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAg                                                                                                                             <  1:1101576/151‑1 (MQ=255)
                     tGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATTGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGa                                                                                                                            >  1:252711/1‑151 (MQ=255)
                       cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCGGGCGTTGGAAATGCTTCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATa                                                                                                                          <  1:1326025/151‑1 (MQ=255)
                         ggCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAGGGGGTTCTATTTTGAAGCCGGGGCGGATGAAGATaca                                                                                                                        >  1:1973916/1‑151 (MQ=255)
                         ggCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAACGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATaca                                                                                                                        >  1:1973972/1‑151 (MQ=255)
                                 atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                                                                                >  1:518628/1‑151 (MQ=255)
                                 atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGGTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                                                                                >  1:575749/1‑151 (MQ=255)
                                     aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgcg                                                                                                            >  2:218565/1‑151 (MQ=255)
                                     aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgcg                                                                                                            >  1:1552925/1‑151 (MQ=255)
                                                 ggCGAACCGCGAATGCGTACCGATGGCCGAACTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaa                                                                                                >  2:5305/1‑151 (MQ=255)
                                                   cGAACGGTGAAGGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAc                                                                                              >  2:696037/1‑151 (MQ=255)
                                                   cGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAAc                                                                                              <  2:924207/151‑1 (MQ=255)
                                                   cGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAc                                                                                              >  2:1477111/1‑151 (MQ=255)
                                                   cGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAc                                                                                              >  2:722836/1‑151 (MQ=255)
                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAActct                                                                                           <  1:1968763/151‑1 (MQ=255)
                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                                                                           <  1:1737458/151‑1 (MQ=255)
                                                       ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                                                                                          <  1:1248659/151‑1 (MQ=255)
                                                        cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                         >  1:422207/1‑151 (MQ=255)
                                                         gcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                                                        <  2:404753/151‑1 (MQ=255)
                                                         gcgAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcgc                                                                                       <  1:1169894/151‑1 (MQ=255)
                                                               gCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACAt                                                                                  <  1:1593855/151‑1 (MQ=255)
                                                               gCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAt                                                                                  <  1:1155519/151‑1 (MQ=255)
                                                                cGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtg                                                                                 >  1:1508658/1‑151 (MQ=255)
                                                                cGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtg                                                                                 >  1:489260/1‑151 (MQ=255)
                                                                 gTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                                                >  1:958272/1‑151 (MQ=255)
                                                                 gTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                                                >  1:1567872/1‑151 (MQ=255)
                                                                    ccGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGCTGAAGATACACACTTCCGCGCGACCCGCCTGGAAACTCTGCGCAACAtggtgg                                                                             >  1:903808/1‑151 (MQ=255)
                                                                    ccGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                                                             <  2:797766/151‑1 (MQ=255)
                                                                     cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggc                                                                            <  2:489252/151‑1 (MQ=255)
                                                                      gATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg                                                                           <  1:480104/151‑1 (MQ=255)
                                                                       aTGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                                                                          <  2:699173/151‑1 (MQ=255)
                                                                           ccGATCTGGCAGGGGAACAACTGCTGATGCTGGAAGCTGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACCTGGTGGCGGCAgg                                                                      <  1:556335/151‑1 (MQ=255)
                                                                                 tGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCgg                                                                <  2:79034/151‑1 (MQ=255)
                                                                                 gggCAGGGGAAAACCTGCTGATGCTGGAAGGGGGGCACTGTTTGCGCGATCAGGCGATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGAGACACACTGCCGCGCGGCCAGCCTGGAAACTCTGCGCAACATGGTGGCGGGAGGTAGCgg                                                                <  2:318649/150‑1 (MQ=255)
                                                                                  ggCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                                               >  1:1707272/1‑151 (MQ=255)
                                                                                   gCAGGGGAAAATCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGCAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGa                                                              <  2:1902221/151‑1 (MQ=255)
                                                                                    cAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGaa                                                             >  2:1688126/1‑150 (MQ=255)
                                                                                    cAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                                             >  1:1422165/1‑151 (MQ=255)
                                                                                     aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                                            <  1:1035288/151‑1 (MQ=255)
                                                                                     aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                                            <  1:1035310/151‑1 (MQ=255)
                                                                                        ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                         <  2:194573/151‑1 (MQ=255)
                                                                                        ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                         >  2:720725/1‑151 (MQ=255)
                                                                                        ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGACGAAGATACACACTGCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                         <  2:1931330/151‑1 (MQ=255)
                                                                                        ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCC‑GGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                        <  1:1967869/151‑1 (MQ=255)
                                                                                         gAAAAGCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCGATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                        <  2:1046080/151‑1 (MQ=255)
                                                                                         gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                        <  1:1951002/151‑1 (MQ=255)
                                                                                         gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                        <  1:1694776/151‑1 (MQ=255)
                                                                                             aaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACt                                                    <  1:784860/151‑1 (MQ=255)
                                                                                             aaCTGCTGATGCGGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACt                                                    <  2:584843/151‑1 (MQ=255)
                                                                                              aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTg                                                   <  2:518620/151‑1 (MQ=255)
                                                                                              aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTg                                                   <  2:1758730/151‑1 (MQ=255)
                                                                                               ctgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                                                  >  2:1867462/1‑151 (MQ=255)
                                                                                                tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGcc                                                 >  2:847979/1‑151 (MQ=255)
                                                                                                tgctgATGCTGGAAGATGGCCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGcc                                                 >  1:1861995/1‑151 (MQ=255)
                                                                                                      tGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgct                                           >  2:21501/1‑151 (MQ=255)
                                                                                                       gCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctg                                          >  1:1064644/1‑151 (MQ=255)
                                                                                                        cTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                                         >  1:189225/1‑151 (MQ=255)
                                                                                                        cTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                                         >  1:871533/1‑151 (MQ=255)
                                                                                                         tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggc                                        <  1:1675280/151‑1 (MQ=255)
                                                                                                          ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggct                                       >  2:1337424/1‑151 (MQ=255)
                                                                                                           gAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggctt                                      <  1:736753/151‑2 (MQ=255)
                                                                                                                tGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGCCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccg                                 >  2:956682/1‑151 (MQ=255)
                                                                                                                 ggTCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggctacaccac                                >  2:1935097/1‑144 (MQ=255)
                                                                                                                   tCACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGccgccg                              >  1:1933129/1‑151 (MQ=255)
                                                                                                                    cACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCgg                             <  2:1976003/151‑1 (MQ=255)
                                                                                                                    cACTGTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCGGGCTGTGCCGCCgg                             >  2:506523/1‑151 (MQ=255)
                                                                                                                        gTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                         <  1:86792/151‑1 (MQ=255)
                                                                                                                        gTTTGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                         <  1:689080/151‑1 (MQ=255)
                                                                                                                          ttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCa                       <  1:1401073/151‑1 (MQ=255)
                                                                                                                           tGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaa                      >  2:2032417/1‑151 (MQ=255)
                                                                                                                           tGCGCGATCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaa                      >  2:935081/1‑151 (MQ=255)
                                                                                                                                 aTCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGa                >  2:1410244/1‑151 (MQ=255)
                                                                                                                                  tCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt               <  2:1064628/151‑1 (MQ=255)
                                                                                                                                  tCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt               <  2:1973946/151‑1 (MQ=255)
                                                                                                                                  tCAGGCAATGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCGGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGAt               <  2:1973890/151‑1 (MQ=255)
                                                                                                                                   cAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATg              >  1:11314/1‑151 (MQ=255)
                                                                                                                                          tGGGTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgt       >  1:1203425/1‑151 (MQ=255)
                                                                                                                                            ggTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGttt     >  1:1676712/1‑151 (MQ=255)
                                                                                                                                             gTTTCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTa    <  2:928417/151‑1 (MQ=255)
                                                                                                                                              tttCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGACGGAGCGCAAACGCGATGGGGTTGTTTAt   >  1:2021466/1‑151 (MQ=255)
                                                                                                                                               ttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCAGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATc  <  1:1327054/151‑1 (MQ=255)
                                                                                                                                               ttCTATTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATc  >  2:547283/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATC  >  NC_000913/4158965‑4159258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑