mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Ferulic-2_S24_L001_R1_001.good.fq | 207,833 | 30,297,993 | 100.0% | 145.8 bases | 148 bases | 96.5% |
| errors | Ferulic-2_S24_L001_R2_001.good.fq | 207,833 | 30,273,172 | 100.0% | 145.7 bases | 148 bases | 95.9% |
| errors | Ferulic-2_S24_L002_R1_001.good.fq | 192,314 | 28,026,870 | 100.0% | 145.7 bases | 148 bases | 96.5% |
| errors | Ferulic-2_S24_L002_R2_001.good.fq | 192,314 | 28,004,731 | 100.0% | 145.6 bases | 148 bases | 95.7% |
| errors | Ferulic-2_S24_L003_R1_001.good.fq | 195,296 | 28,469,017 | 100.0% | 145.8 bases | 148 bases | 96.4% |
| errors | Ferulic-2_S24_L003_R2_001.good.fq | 195,296 | 28,444,444 | 100.0% | 145.6 bases | 148 bases | 95.9% |
| errors | Ferulic-2_S24_L004_R1_001.good.fq | 183,753 | 26,778,578 | 100.0% | 145.7 bases | 148 bases | 96.4% |
| errors | Ferulic-2_S24_L004_R2_001.good.fq | 183,753 | 26,756,524 | 100.0% | 145.6 bases | 148 bases | 95.5% |
| total | 1,558,392 | 227,051,329 | 100.0% | 145.7 bases | 148 bases | 96.1% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 35.3 | 1.9 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 22007 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1263 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.062 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.89582 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 06:01:04 19 Feb 2020 | 06:01:29 19 Feb 2020 | 25 seconds |
| Read alignment to reference genome | 06:01:29 19 Feb 2020 | 06:04:51 19 Feb 2020 | 3 minutes 22 seconds |
| Preprocessing alignments for candidate junction identification | 06:04:51 19 Feb 2020 | 06:05:13 19 Feb 2020 | 22 seconds |
| Preliminary analysis of coverage distribution | 06:05:13 19 Feb 2020 | 06:06:04 19 Feb 2020 | 51 seconds |
| Identifying junction candidates | 06:06:04 19 Feb 2020 | 06:06:23 19 Feb 2020 | 19 seconds |
| Re-alignment to junction candidates | 06:06:23 19 Feb 2020 | 06:07:37 19 Feb 2020 | 1 minute 14 seconds |
| Resolving best read alignments | 06:07:37 19 Feb 2020 | 06:08:11 19 Feb 2020 | 34 seconds |
| Creating BAM files | 06:08:11 19 Feb 2020 | 06:08:59 19 Feb 2020 | 48 seconds |
| Tabulating error counts | 06:08:59 19 Feb 2020 | 06:09:15 19 Feb 2020 | 16 seconds |
| Re-calibrating base error rates | 06:09:15 19 Feb 2020 | 06:09:17 19 Feb 2020 | 2 seconds |
| Examining read alignment evidence | 06:09:17 19 Feb 2020 | 06:12:45 19 Feb 2020 | 3 minutes 28 seconds |
| Polymorphism statistics | 06:12:45 19 Feb 2020 | 06:12:46 19 Feb 2020 | 1 second |
| Output | 06:12:46 19 Feb 2020 | 06:13:14 19 Feb 2020 | 28 seconds |
| Total | 12 minutes 10 seconds | ||
