New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 2525575 =131 (1.050)3 (0.030) 3/228 NT 2.8% intergenic (‑147/+83) sirB/ytmI Sirohydrochlorin ferrochelatase/putative N‑acetyltransferase YtmI
?USA300TCH1516_ALE 2525640 = 95 (0.860)intergenic (‑212/+18) sirB/ytmI Sirohydrochlorin ferrochelatase/putative N‑acetyltransferase YtmI
Rejected: Frequency below/above cutoff threshold.

GGTGTGATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/2525729‑2525575
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atatatcggtcgcttGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTT  >  USA300TCH1516_ALE/2525640‑2525778
                                                                                                                                                                                                                                                                                                      
GGTGTGATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCA                                                                                                                                                           >  4:50545/1‑141
GGTGTGATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCA                                                                                                                                                           >  1:677154/1‑141
 GTGTGATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCAT                                                                                                                                                          <  3:34737/141‑1
  TGTGATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATA                                                                                                                                                         >  1:739193/1‑141
     GATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATAT                                                                                                                                                      >  2:148040/1‑141
        CATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTGGAGTAGGGATTCATATATCGG                                                                                                                                                   <  1:660377/141‑1
         ATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTGATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGT                                                                                                                                                  <  1:847600/141‑1
         ATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGT                                                                                                                                                  <  1:300959/141‑1
          TGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTC                                                                                                                                                 >  4:307162/1‑141
          TGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTC                                                                                                                                                 >  1:310838/1‑141
          TGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTC                                                                                                                                                 <  2:350996/141‑1
           GCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCCACCACCATTTTAAGCTAATAATATCCACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCG                                                                                                                                                <  3:130456/141‑1
           GCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCG                                                                                                                                                >  1:1073304/1‑141
           GCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCG                                                                                                                                                <  2:339991/141‑1
            CGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGC                                                                                                                                               <  1:438880/141‑1
            CGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGC                                                                                                                                               <  2:108108/141‑1
            CGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGC                                                                                                                                               <  2:876881/141‑1
             GCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCT                                                                                                                                              <  1:925447/141‑1
              CAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTT                                                                                                                                             <  2:978093/141‑1
              CAAAATATTGATACCCCAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTT                                                                                                                                             <  1:326688/141‑1
                                                                                                CCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATG                                                           >  4:257849/1‑141
                                                                                                  GATATATCAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGgt                                                         >  3:353241/1‑139
                                                                                                            TGCTTACTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAA                                               >  1:846244/1‑141
                                                                                                                                            ATATATCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATAT               >  1:774780/1‑141
                                                                                                                                            ATATATCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATAT               >  2:449450/1‑141
                                                                                                                                            ATATATCGGTCGCTTGTAGGGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATAT               >  2:449430/1‑141
                                                                                                                                               TATCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTC            >  2:105652/1‑141
                                                                                                                                                 TCGGTCGCTTGTAGTGTATATTCTTATCTTAAAATCGTGGTTGGCCTAATATTGTTACCTCAAACCGCTCGGGTATCAATACTTTGCGCATGATCACACCTACATCGCCATCATCATTTTCATGTTCGCTGTATACTTCAT          >  4:446797/1‑141
                                                                                                                                                 TCGGTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCAT          >  2:171660/1‑141
                                                                                                                                                    GTCGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAAC       <  2:300959/141‑1
                                                                                                                                                      CGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCT     <  2:31824/141‑1
                                                                                                                                                      CGCTTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCT     <  1:657929/141‑1
                                                                                                                                                         TTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTT  >  2:563306/1‑141
                                                                                                                                                         TTGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTT  <  4:423850/141‑1
                                                                                                                                                                                                                                                                                                      
GGTGTGATCATGCGCAAAGTATTGATACCCGAGCGCTTTGACGAAACAATATTAGGCCAACCACCATTTTAAGATAATAATATACACTACAAGCGACCGATATATTAATGCTTGCTCAAATAAATGTTGAGTAGGGATTCATATATCGGTCGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/2525729‑2525575
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atatatcggtcgcttGTAGTGTATATTATTATCTTAAAATGGTGGTTGGCCTAATATTGTTTCGTCAAAGCGCTCGGGTATCAATACTTTGCGCATGATCACACCTAAATCGCCATCATCATTTTCATGTTCGCTGTATATTTCATAACCTCTT  >  USA300TCH1516_ALE/2525640‑2525778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.