Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,310,096 G→A 6.4% intergenic (+121/‑44) cinA → / → recA Putative competence‑damage inducible protein/Protein RecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,310,0960GA 6.4% 315.9 / 5.8 103intergenic (+121/‑44)cinA/recAPutative competence‑damage inducible protein/Protein RecA
Reads supporting (aligned to +/- strand):  ref base G (50/46);  new base A (2/5);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GGCTTAGAACAACAAATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAG  >  USA300TCH1516_ALE/1309956‑1310236
                                                                                                                                            |                                                                                                                                            
ggCTTAGAACAACAAATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTgg                                                                                                                                              <  2:304214/141‑1 (MQ=255)
  cTTAGAACAACAAATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTa                                                                                                                                            >  1:559884/1‑141 (MQ=255)
     aGAACAACAAATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGATTCCTTGTATAGCATTGGTAAGa                                                                                                                                         >  2:318859/1‑141 (MQ=255)
     aGAACAACAAATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGa                                                                                                                                         >  2:633831/1‑141 (MQ=255)
         caacaaATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAAt                                                                                                                                     <  1:578900/141‑1 (MQ=255)
          aacaaATTAATTGTATTATGGATAAAAATATAAGCACGTTTGTAAGATTTTGCGTTTTGATTTCAAGATTTTATATGAACAAATATTCACAAAACTCTTGTATTTTATTTTGAATCCTTGTATAGTATTGATAAGATAAtt                                                                                                                                    <  4:325597/141‑1 (MQ=255)
          aacaaATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTAAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAAtt                                                                                                                                    >  4:28248/1‑141 (MQ=255)
                ttaattGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGa                                                                                                                              >  1:230455/1‑141 (MQ=255)
                  aattGTATTATCGATAAAAATATAAGCACGTTTTTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAAGATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATa                                                                                                                            <  4:293704/141‑1 (MQ=255)
                  aattGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATa                                                                                                                            <  3:37059/141‑1 (MQ=255)
                   attGTATTATCGATAAAAATATAAGCAAGTTAGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAg                                                                                                                           >  2:272802/1‑141 (MQ=255)
                      gTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCaa                                                                                                                        <  1:1083197/141‑1 (MQ=255)
                         ttatCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAAttt                                                                                                                     <  2:497551/141‑1 (MQ=255)
                         ttatCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAAttt                                                                                                                     <  2:840573/141‑1 (MQ=255)
                         ttatCGATAAAAATATAAGCACGTTTGTTCGTTTGTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAAttt                                                                                                                     >  2:49397/1‑141 (MQ=255)
                             cGATAAAAATATAATCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGGAAGATAATTAAAAGATAGCAATTTCAAt                                                                                                                 <  2:973115/141‑1 (MQ=255)
                             cGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTAAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAAt                                                                                                                 <  2:76269/141‑1 (MQ=255)
                                tAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTag                                                                                                              >  2:577513/1‑141 (MQ=255)
                                    aaTATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTaggagg                                                                                                          <  2:596588/141‑1 (MQ=255)
                                    aaTATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTaggagg                                                                                                          <  4:61063/141‑1 (MQ=255)
                                     atatAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCTCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGt                                                                                                         >  1:652919/1‑141 (MQ=255)
                                      tataAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGtc                                                                                                        <  1:550011/141‑1 (MQ=255)
                                       ataAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGtct                                                                                                       >  2:963824/1‑141 (MQ=255)
                                       ataAGCACGTCTGTTCGTTCACGCGCTTTGATTTCAAGATCTTATACGAACAAACATCCGCCAAACACTCGTATTTTATTTTGAATCCTTGCATAGCATCGGTAACATAATTAACAGACAGCCCTTTCCATCAGCAGGtcc                                                                                                       >  2:608534/1‑140 (MQ=255)
                                         aaGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCg                                                                                                     >  3:433244/1‑141 (MQ=255)
                                          aGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGc                                                                                                    >  2:252176/1‑141 (MQ=255)
                                             aCGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCttt                                                                                                 >  2:285009/1‑141 (MQ=255)
                                               gTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGATAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTgg                                                                                               <  2:115254/141‑1 (MQ=255)
                                               gTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGATAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTgg                                                                                               <  1:892606/141‑1 (MQ=255)
                                                tttGTTCGTTTTTTTTTTTTTTTTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATCGTATTTGTAAGATAATTAAAAGATAGCAATTTCATTTAGTAGGTCTCGCTTTGGa                                                                                              >  2:229754/1‑141 (MQ=255)
                                                tttGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGa                                                                                              <  3:153515/141‑1 (MQ=255)
                                                  tGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATa                                                                                            >  2:291762/1‑141 (MQ=255)
                                                   gTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATaa                                                                                           >  1:167369/1‑141 (MQ=255)
                                                    ttCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAAc                                                                                          >  2:1015581/1‑141 (MQ=255)
                                                     tCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACg                                                                                         <  4:182280/141‑1 (MQ=255)
                                                      cGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGATAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTTTCGCTTTGGATAACGa                                                                                        >  4:312084/1‑141 (MQ=255)
                                                        tttttGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATc                                                                                      >  3:352487/1‑141 (MQ=255)
                                                            tGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCa                                                                                  <  1:97239/141‑1 (MQ=255)
                                                            tGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCa                                                                                  <  2:907858/141‑1 (MQ=255)
                                                            tGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCa                                                                                  <  1:650312/141‑1 (MQ=255)
                                                             gCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCaa                                                                                 <  1:737887/141‑1 (MQ=255)
                                                             gCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCaa                                                                                 <  2:1040280/141‑1 (MQ=255)
                                                             gCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCaa                                                                                 >  4:49176/1‑141 (MQ=255)
                                                             gCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCaa                                                                                 <  1:499332/141‑1 (MQ=255)
                                                                ttttGATTTCAAGATTTTATACGAACAAATATTCGCAAAGCACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGAGCGTCaaaaa                                                                              <  1:955084/141‑1 (MQ=255)
                                                                ttttGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCaaaaa                                                                              >  2:245707/1‑141 (MQ=255)
                                                                      tttCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTa                                                                        >  2:570917/1‑141 (MQ=255)
                                                                      tttCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTa                                                                        >  4:36004/1‑141 (MQ=255)
                                                                        tCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGa                                                                      >  1:819290/1‑141 (MQ=255)
                                                                         cAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGAt                                                                     >  1:670563/1‑141 (MQ=255)
                                                                            gATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACa                                                                  <  1:841483/141‑1 (MQ=255)
                                                                            gATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACa                                                                  <  3:66762/141‑1 (MQ=255)
                                                                            gATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGAAAAAAAAAAAAAAAAAGCTTTAGATACa                                                                  <  2:279797/141‑1 (MQ=255)
                                                                              ttttATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGt                                                                <  1:436965/141‑1 (MQ=255)
                                                                              ttttATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGt                                                                >  1:192514/1‑141 (MQ=255)
                                                                               tttATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTa                                                               >  4:237104/1‑141 (MQ=255)
                                                                                ttATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTaa                                                              >  1:849854/1‑141 (MQ=255)
                                                                                ttATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTaa                                                              >  1:677709/1‑141 (MQ=255)
                                                                                ttATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTaa                                                              >  3:450077/1‑141 (MQ=255)
                                                                                 tataCGAACAAATATTCGCAACACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAAt                                                             >  3:217222/1‑141 (MQ=255)
                                                                                 tataCGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAAt                                                             <  3:124446/141‑1 (MQ=255)
                                                                                 tataCGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAAt                                                             <  1:1030084/141‑1 (MQ=255)
                                                                                 tataCGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAAt                                                             <  2:1045398/141‑1 (MQ=255)
                                                                                 tagaCGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAAACCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTAAATTAGGAGGTCTCGCTTTGGAAAACGATCGTCAAAAAGCTTTAGATACAGTAAt                                                             <  2:866948/138‑1 (MQ=255)
                                                                                  ataCGAACAAATATTCTCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAAtt                                                            >  2:962550/1‑141 (MQ=255)
                                                                                   taCGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTa                                                           >  2:996171/1‑141 (MQ=255)
                                                                                   taCGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTa                                                           >  2:705505/1‑141 (MQ=255)
                                                                                    aagAAAAAATATTCGCAAAAGACTTGTATTTTATTTTGAATCGTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAAAGAAAGTAAAAAAGCTTTAGATACAGTAATTaa                                                          <  2:1021259/139‑1 (MQ=255)
                                                                                     cGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTaaa                                                         >  2:315054/1‑141 (MQ=255)
                                                                                       aaCAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTaaaaa                                                       <  1:632713/141‑1 (MQ=255)
                                                                                         cAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAata                                                     <  1:725507/141‑1 (MQ=255)
                                                                                             tatTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGga                                                 <  1:427110/141‑1 (MQ=255)
                                                                                               ttCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGgaga                                               <  1:624944/141‑1 (MQ=255)
                                                                                                 cGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGTTACAGTAATTAAAAATATGGAGaaa                                             >  1:884960/1‑141 (MQ=255)
                                                                                                  gCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAAt                                            >  1:952005/1‑141 (MQ=255)
                                                                                                     aaaCACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCtt                                         <  1:424258/141‑1 (MQ=255)
                                                                                                       acacTTGTATTTTATTTTGAATCCTTGTATAGTATTGATAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTc                                       >  2:821805/1‑141 (MQ=255)
                                                                                                           ttGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTa                                   <  4:366142/141‑1 (MQ=255)
                                                                                                            tGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTaa                                  >  4:420818/1‑141 (MQ=255)
                                                                                                              tattttatttTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAg                                >  1:730672/1‑141 (MQ=255)
                                                                                                               attttattttGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAgg                               >  1:905901/1‑141 (MQ=255)
                                                                                                                      tttGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTa                        >  1:339247/1‑141 (MQ=255)
                                                                                                                       ttGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTaa                       <  1:608700/141‑1 (MQ=255)
                                                                                                                       ttGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTaa                       <  1:991799/141‑1 (MQ=255)
                                                                                                                        tGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAAt                      <  4:316564/141‑1 (MQ=255)
                                                                                                                        tGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAAt                      <  2:169769/141‑1 (MQ=255)
                                                                                                                        tGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAAt                      <  2:196503/141‑1 (MQ=255)
                                                                                                                        tGAATCCTTGTATAGTATTGATAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAAt                      <  3:327857/141‑1 (MQ=255)
                                                                                                                         gAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATg                     <  2:16632/141‑1 (MQ=255)
                                                                                                                              cTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGtt                >  2:435453/1‑141 (MQ=255)
                                                                                                                              cTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGtt                >  2:435584/1‑141 (MQ=255)
                                                                                                                                tGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTgg              <  1:188677/141‑1 (MQ=255)
                                                                                                                                   ataGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGc                            <  4:83236/124‑1 (MQ=255)
                                                                                                                                   ataGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGc                            >  3:83236/1‑124 (MQ=255)
                                                                                                                                   ataGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTg           <  1:337896/141‑1 (MQ=255)
                                                                                                                                   ataGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATGAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTg           >  2:434511/1‑141 (MQ=255)
                                                                                                                                           tgTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCaa                                                                                 >  1:700744/2‑63 (MQ=255)
                                                                                                                                           ggTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCAGCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTGACAAtata   <  1:1053074/141‑1 (MQ=255)
                                                                                                                                            gTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAg  <  2:673152/141‑1 (MQ=255)
                                                                                                                                            gTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAg  <  1:75743/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GGCTTAGAACAACAAATTAATTGTATTATCGATAAAAATATAAGCACGTTTGTTCGTTTTTGCGTTTTGATTTCAAGATTTTATACGAACAAATATTCGCAAAACACTTGTATTTTATTTTGAATCCTTGTATAGTATTGGTAAGATAATTAAAAGATAGCAATTTCAATTAGGAGGTCTCGCTTTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTCGGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAG  >  USA300TCH1516_ALE/1309956‑1310236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: