Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,315,802 C→T 5.1% L362L (CTA→TTA)  korA → 2‑oxoglutarate oxidoreductase subunit KorA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,315,8020CT 5.1% 371.5 / 4.7 117L362L (CTA→TTA) korA2‑oxoglutarate oxidoreductase subunit KorA
Reads supporting (aligned to +/- strand):  ref base C (69/41);  new base T (4/2);  total (73/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 117 total aligned reads displayed.

AGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGC  >  USA300TCH1516_ALE/1315663‑1315941
                                                                                                                                           |                                                                                                                                           
aGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCt                                                                                                                                            <  2:518519/141‑1 (MQ=255)
 gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                                                                                                           >  1:1049628/1‑141 (MQ=255)
 gATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATAGTTGTAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTa                                                                                                                                           >  4:304044/1‑141 (MQ=255)
 aaTTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTa                                                                                                                                           <  2:234276/140‑1 (MQ=255)
    ttAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGt                                                                                                                                        <  1:516025/141‑1 (MQ=255)
    ttAATGCAAATGATTTATGGTACACATAGAGAAAAAAAAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGt                                                                                                                                        <  2:406984/141‑1 (MQ=255)
        tGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAtt                                                                                                                                    <  1:291573/141‑1 (MQ=255)
         gCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                                                                                                   >  1:290574/1‑141 (MQ=255)
         gCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                                                                                                   >  2:489954/1‑141 (MQ=255)
         gCAAATGATTTATAGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGAttt                                                                                                                                   <  4:281456/141‑1 (MQ=255)
          cAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAANTGATTTg                                                                                                                                  >  2:813906/1‑141 (MQ=255)
           aaaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGc                                                                                                                                 >  1:708439/1‑141 (MQ=255)
            aaTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCa                                                                                                                                <  2:595032/141‑1 (MQ=255)
             aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                                                                                               <  2:617741/141‑1 (MQ=255)
             aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                                                                                               >  2:99457/1‑141 (MQ=255)
             aTGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCaa                                                                                                                               >  2:608128/1‑141 (MQ=255)
               gATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGACGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAAtt                                                                                                                             >  2:1000374/1‑141 (MQ=255)
                aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                                                                                            >  2:467539/1‑141 (MQ=255)
                aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                                                                                            <  1:543867/141‑1 (MQ=255)
                aTTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTa                                                                                                                            <  1:543862/141‑1 (MQ=255)
                  ttATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATc                                                                                                                          >  3:280094/1‑141 (MQ=255)
                    aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                                                        >  2:917326/1‑141 (MQ=255)
                    aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                                                        <  1:806693/141‑1 (MQ=255)
                    aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                                                        >  3:227245/1‑141 (MQ=255)
                    aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                                                        >  1:62746/1‑141 (MQ=255)
                    aTGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCtt                                                                                                                        >  3:72414/1‑141 (MQ=255)
                     tGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCttt                                                                                                                       >  2:848184/1‑141 (MQ=255)
                        tACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                                                                                                                    <  2:891681/141‑1 (MQ=255)
                        tACACATGGTGATATTCCAAAAATTGTTGTAAACACGACATATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAgg                                                                                                                    >  1:29959/1‑141 (MQ=255)
                           acaTGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaa                                                                                                                 <  2:252249/141‑1 (MQ=255)
                              tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                                                                                                              >  2:887063/1‑141 (MQ=255)
                              tGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaaca                                                                                                              >  2:488376/1‑141 (MQ=255)
                                 tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                                                                                                           >  3:105840/1‑141 (MQ=255)
                                 tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                                                                                                           >  3:105818/1‑141 (MQ=255)
                                 tGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTaaacaaac                                                                                                           >  3:367160/1‑141 (MQ=255)
                                  gATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACt                                                                                                          >  2:176378/1‑141 (MQ=255)
                                    tatTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGt                                                                                                        >  3:286662/1‑141 (MQ=255)
                                      ttCCAAAAAATGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTg                                                                                                      >  1:956513/1‑141 (MQ=255)
                                       tCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGa                                                                                                     <  2:449140/141‑1 (MQ=255)
                                          aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa                                                                                                  >  2:13216/1‑141 (MQ=255)
                                          aaaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaa                                                                                                  >  2:767645/1‑141 (MQ=255)
                                           aaaaTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGaaaaa                                                                                                 >  1:569208/1‑141 (MQ=255)
                                              aTTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTa                                                                                              <  1:770076/141‑1 (MQ=255)
                                               ttgttgTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAg                                                                                             <  3:258240/141‑1 (MQ=255)
                                                  ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                                                          >  2:377769/1‑141 (MQ=255)
                                                  ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                                                          >  2:489442/1‑141 (MQ=255)
                                                  ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                                                          >  2:49829/1‑141 (MQ=255)
                                                  ttgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGAtt                                                                                          <  3:176745/141‑1 (MQ=255)
                                                   tgtAGCACCAACTGATGCAGAAGATGCATTTTATTTAACTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta                                                                                         >  2:822190/1‑141 (MQ=255)
                                                   tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta                                                                                         >  3:296468/1‑141 (MQ=255)
                                                   tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta                                                                                         >  2:629588/1‑141 (MQ=255)
                                                   tgtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATta                                                                                         >  2:707619/1‑141 (MQ=255)
                                                    gtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGTTtat                                                                                        >  3:169421/1‑141 (MQ=255)
                                                    gtAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATtat                                                                                        >  2:172169/1‑141 (MQ=255)
                                                             aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt                                                                               >  2:837146/1‑141 (MQ=255)
                                                             aCAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTAtt                                                                               >  4:337128/1‑141 (MQ=255)
                                                              cAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTg                                                                              >  3:111119/1‑141 (MQ=255)
                                                                  tGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAAt                                                                          <  1:435826/141‑1 (MQ=255)
                                                                    cAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTa                                                                        >  1:111159/1‑141 (MQ=255)
                                                                       aagaTGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAAc                                                                     <  3:13294/141‑1 (MQ=255)
                                                                           tGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtgg                                                                 >  3:80883/1‑141 (MQ=255)
                                                                            gCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtggt                                                                <  3:146588/141‑1 (MQ=255)
                                                                             cATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACgtggtg                                                               >  1:737850/1‑141 (MQ=255)
                                                                                  tatttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATc                                                          >  4:113045/1‑141 (MQ=255)
                                                                                    tttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCAt                                                        <  2:938611/141‑1 (MQ=255)
                                                                                    tttaACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCAt                                                        >  3:237674/1‑141 (MQ=255)
                                                                                        aCTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTg                                                                              <  1:1016607/115‑1 (MQ=255)
                                                                                        aCTATGGAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTg                                                                              >  2:1016607/1‑115 (MQ=255)
                                                                                        aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa                                                    <  4:14641/141‑1 (MQ=255)
                                                                                        aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa                                                    >  1:1087026/1‑141 (MQ=255)
                                                                                        aCTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCaa                                                    >  3:241745/1‑141 (MQ=255)
                                                                                              gAAGCCTTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTGAAATTAAACGAGGTGAAATCATTCAATCTGat                                              >  1:502200/1‑141 (MQ=255)
                                                                                              gAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGat                                              >  2:102015/1‑141 (MQ=255)
                                                                                              gAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTACTGAAATTAAACGTGGTGAAATCATTCAATCTGat                                              >  1:1003880/1‑141 (MQ=255)
                                                                                                tgtatttattttaGTATAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGTAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATATTCGTATTGAAATTAAACGTTGTGAAATCATTCAATCTGatat                                            <  1:610527/138‑1 (MQ=255)
                                                                                                aacaataaatttaAAAAAACAATATCAATACCCTTATATAGTTATAAGTAATTTGCAATTATCTTTAGGTAAAAAAAAAATAAAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGatat                                            <  4:416118/134‑1 (MQ=255)
                                                                                                aGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGatat                                            <  3:284211/141‑1 (MQ=255)
                                                                                                    tttaatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGaa                                        >  3:7613/1‑141 (MQ=255)
                                                                                                     ttaatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAAc                                       >  2:624611/1‑141 (MQ=255)
                                                                                                       aatttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGt                                     >  1:917698/1‑141 (MQ=255)
                                                                                                         tttaGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGCTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTga                                   <  1:428105/141‑1 (MQ=255)
                                                                                                             gCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCTATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTgaagaa                               <  1:854559/141‑1 (MQ=255)
                                                                                                                 aaCAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAgatg                           >  1:64606/1‑141 (MQ=255)
                                                                                                                      tatCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATaaa                      >  1:1048493/1‑141 (MQ=255)
                                                                                                                        tCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAgg                    >  2:711679/1‑141 (MQ=255)
                                                                                                                         cAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGt                   <  4:286662/141‑1 (MQ=255)
                                                                                                                            tGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTCGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGatat                >  2:4856/1‑141 (MQ=255)
                                                                                                                            tGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTGAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTAt                >  1:19084/1‑141 (MQ=255)
                                                                                                                              cccTGTTAAAGTTCAAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTAttt              <  1:346241/141‑1 (MQ=255)
                                                                                                                               ccTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTc             >  1:267247/1‑141 (MQ=255)
                                                                                                                                cTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCa            >  2:1020868/1‑141 (MQ=255)
                                                                                                                                 tGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAGAGGTTATTTCaa           <  4:148287/141‑1 (MQ=255)
                                                                                                                                   ttATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGc         <  2:872809/141‑1 (MQ=255)
                                                                                                                                   ttATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGc         <  1:498966/141‑1 (MQ=255)
                                                                                                                                    tataGTTTTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGCATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCg        <  3:194856/141‑1 (MQ=255)
                                                                                                                                    tataGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCg        <  2:115710/141‑1 (MQ=255)
                                                                                                                                     ataGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGt       >  1:409317/1‑141 (MQ=255)
                                                                                                                                     ataGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGt       <  1:608128/141‑1 (MQ=255)
                                                                                                                                        gTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGACAAAGGTTATTTCAAGCGCTAt    >  2:480671/1‑141 (MQ=255)
                                                                                                                                          tCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGc  >  2:887943/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGATTTAATGCAAATGATTTATGGTACACATGGTGATATTCCAAAAATTGTTGTAGCACCAACAGATGCAGAAGATGCATTTTATTTAACTATGGAAGCATTTAATTTAGCAGAACAATATCAATGCCCTGTTATAGTTCTAAGTGATTTGCAATTATCTTTAGGTAAACAAACTGTTGAAAAATTAGATTATAATCGTATTGAAATTAAACGTGGTGAAATCATTCAATCTGATATTGAACGTGAAGAAGATGATAAAGGTTATTTCAAGCGTTATGC  >  USA300TCH1516_ALE/1315663‑1315941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: